Results 51 to 60 of about 17,987 (161)

Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases. [PDF]

PLoS ONE, 2012
The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies.
Priscila O de Giuseppe, Nadia H Martins, Andreia N Meza, Camila R dos Santos, Humberto D'Muniz Pereira, Mario T Murakami   +5 more
doaj   +1 more source

Tri-Cyclic Nucleobase Analogs and Their Ribosides as Substrates of Purine-Nucleoside Phosphorylases. II Guanine and Isoguanine Derivatives

Molecules, 2019
Etheno-derivatives of guanine, O6-methylguanine, and isoguanine were prepared and purified using standard methods. The title compounds were examined as potential substrates of purine-nucleoside phosphorylases from various sources in the reverse ...
Alicja Stachelska-Wierzchowska, Jacek Wierzchowski, Michał Górka, Agnieszka Bzowska, Beata Wielgus-Kutrowska   +4 more
doaj   +1 more source

Engineering Substrate Promiscuity of Nucleoside Phosphorylase Via an Insertions–Deletions Strategy

JACS Au
Nucleoside phosphorylases (NPs) are the key enzymes in the nucleoside metabolism pathway and are widely employed for the synthesis of nucleoside analogs, which are difficult to access via conventional synthetic methods.
Gaofei Liu, Jialing Wang, Jianlin Chu, Tianyue Jiang, Song Qin, Zhen Gao, Bingfang He   +6 more
semanticscholar   +1 more source

The ligand binding mechanism to purine nucleoside phosphorylase elucidated via molecular dynamics and machine learning

Nature Communications, 2015
The study of biomolecular interactions between a drug and its biological target is of paramount importance for the design of novel bioactive compounds.
S. Decherchi, Anna Berteotti, G. Bottegoni, W. Rocchia, A. Cavalli   +4 more
semanticscholar   +1 more source

Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs

FEBS Open Bio, 2015
Mycoplasmas may colonize tumor tissue in patients. The cytostatic activity of gemcitabine was dramatically decreased inMycoplasma hyorhinis‐infected tumor cell cultures compared with non‐infected tumor cell cultures.
Johan Vande Voorde, Peter Vervaeke, Sandra Liekens, Jan Balzarini   +3 more
doaj   +1 more source

A specific adenosine phosphorylase, distinct from purine nucleoside phosphorylase

FEBS Letters, 1976
Several enzymes, catalyzing the reversible phosphorolysis of purine nucleosides have been described in eucaryotic cells and in micro-organisms [l-6] . The best known is the ‘purine nucleoside phosphorylase’ (EC 2.4.2.1) acting on the nucleosides of hypoxanthine and guanine. Adenosine is not used as substrate by this enzyme [6-81.
SENESI, SONIA, FALCONE, GIUSEPPE, MURA, UMBERTO, SGARRELLA F, IPATA, PIERO LUIGI   +4 more
openaire   +5 more sources

Purine nucleoside phosphorylase (PNP) deficiency: across-the-board severe combined immunodeficiency

Egyptian Journal of Medical Human Genetics
Background Purine nucleoside phosphorylase (PNP) deficiency is a rare, autosomal recessive, inborn error of immunity. It is characterized by progressive immune abnormalities ranging from severe combined immunodeficiency (SCID) to combined ...
Engy A. Chohayeb, Sohilla Lotfy, Rabab E. El Hawary, Safa S. Meshaal, Iman A. Mansour, Nermeen M. Galal, Aisha M. Elmarsafy   +6 more
doaj   +1 more source

Circulating Human Neonatal Naïve B cells are Deficient in CD73 Impairing Purine Salvage

Frontiers in Immunology, 2016
Background: Extracellular purines, in particular adenosine (Ado) and adenosine-triphosphate (ATP), are critical immunoregulatory molecules. Expression and activity of purine ecto-enzymes on B cells in neonatal and adult blood may influence their function
Matthew Aaron Pettengill, Ofer eLevy, Ofer eLevy   +2 more
doaj   +1 more source

Computational quantum chemistry, molecular docking, and ADMET predictions of imidazole alkaloids of Pilocarpus microphyllus with schistosomicidal properties.

PLoS ONE, 2018
Schistosomiasis affects million people and its control is widely dependent on a single drug, praziquantel. Computational chemistry has led to the development of new tools that predict molecular properties related to pharmacological potential.
Jefferson A Rocha, Nayra C S Rego, Bruna T S Carvalho, Francisco I Silva, Jose A Sousa, Ricardo M Ramos, Ionara N G Passos, Josué de Moraes, Jose R S A Leite, Francisco C A Lima   +9 more
doaj   +1 more source

Pediococcus acidilactici GR-5 alleviates hyperuricemia by degrading purine nucleosides and improving gut microbiota metabolism

npj Science of Food
Reducing intestinal absorption by degrading purine nucleosides has been shown to alleviate hyperuricemia (HUA). The probiotic Pediococcus acidilactici GR-5, derived from the traditional food “Jiangshui”, achieved efficient degradation of purine ...
Jing Ji, Zi-Yi An, Aman Khan, Liang Peng, Sourabh Kulshreshtha, El-Sayed Salama, Hui Yun, Pu Liu, Wei-Lin Jin, Xiangkai Li   +9 more
doaj   +1 more source