Catalytic-site design for inverse heavy-enzyme isotope effects in human purine nucleoside phosphorylase. [PDF]
Proc Natl Acad Sci U S A, 2017 Significance Protein design from first principles is developing rapidly for structural elements, binding domains, and protein–protein interactions. Design of structural elements to generate predictable changes in the fundamental properties of enzymatic ...
Harijan RK +4 more
europepmc +2 more sources
Trypanosoma brucei methylthioadenosine phosphorylase protects the parasite from the antitrypanosomal effect of deoxyadenosine [PDF]
, 2016 Trypanosoma brucei causes African sleeping sickness for which no vaccine exists and available treatments are of limited use due to their high toxicity or lack of efficacy. T.
de Koning, Harry P. +4 more
core +1 more source
Pyrazolo[4,3-e][1,2,4]triazines: Purine Analogues with Electronic Absorption in the Visible Region
Molecules, 2005 Synthesis of several pryrazolo[4,3-e][1,2,4]-triazines is described. Theabsorption spectrum of some 5-substituted derivatives was found to extend to the visibleregion.
Jacek Wierzchowski +2 more
doaj +1 more source
Ethenoguanines undergo glycosylation by nucleoside 2'-deoxyribosyltransferases at non-natural sites. [PDF]
PLoS ONE, 2014 Deoxyribosyl transferases and functionally related purine nucleoside phosphorylases are used extensively for synthesis of non-natural deoxynucleosides as pharmaceuticals or standards for characterizing and quantitating DNA adducts.
Wenjie Ye +10 more
doaj +1 more source
Role of HIV RNA structure in recombination and speciation: romping in purine A, keeps HTLV away [PDF]
Microbes and Infection (2014) 16, 96-103, 2013 Extreme enrichment of the human immunodeficiency virus (HIV-1) RNA genome for the purine A parallels the mild purine-loading of the RNAs of most organisms. This should militate against loop-loop "kissing" interactions between the structured viral genome and structured host RNAs, which can generate segments of double-stranded RNA sufficient to trigger ...
arxiv +1 more source
Design and Synthesis of New Modified Flexible Purine Bases as Potential Inhibitors of Human PNP
Molecules, 2023 The great interest in studying the structure of human purine nucleoside phosphorylase (hPNP) and the continued search for effective inhibitors is due to the importance of the enzyme as a target in the therapy of T-cell proliferative diseases. In addition,
Anastasia Khandazhinskaya +7 more
doaj +1 more source
Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36 [PDF]
, 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 was cloned and overexpressed in Escherichia coli. The enzyme was purified fivefold by membrane filtration and ion exchange.
A Bzowska +39 more
core +2 more sources
FAMIN is a multifunctional purine enzyme enabling the purine nucleotide cycle [PDF]
, 2020 Mutations in FAMIN cause arthritis and inflammatory bowel disease in early childhood, and a common genetic variant increases risk for Crohn’s disease and leprosy.
Bradley, Allan +21 more
core +3 more sources
Immunological Investigations, 2019 Purine nucleoside phosphorylase (PNP) deficiency is a rare autosomal recessive primary immunodeficiency disorder characterized by decreased numbers of T-cells, variable B-cell abnormalities, decreased amount of serum uric acid and PNP enzyme activity ...
Saba Fekrvand +4 more
semanticscholar +1 more source
Purine nucleoside phosphorylase from Schistosoma mansoni in complex with ribose-1-phosphate [PDF]
, 2011 A binary complex between a low-molecular-weight purine nucleoside phosphorylase and ribose-1-phosphate is described for the first time and comparisons with known ternary complexes are ...
D’Muniz Pereira, Humberto +2 more
core +2 more sources