Results 1 to 10 of about 10,832 (153)

Development of pyrogen-free utensils for cell culture by combining ozone/hydrogen peroxide mixed gas sterilization with steam-washing technology [PDF]

Regenerative Therapy
Objective: The aim of this study was to develop disposable cell culture utensils with improved cleanliness. Methods: Pyrogen contamination levels were evaluated using a direct human cell-based pyrogen test (HCPT).
Shaimaa Ibrahim, Yasuo Nakamura, Emi Sugizaki, Genki Koyama, Zhiping Chen, Shiori Tamura, Haruka Shinohara, Kenya Sato, Yoh-ichi Tagawa, Erika Sasaki, Hiroyuki Tomari, Masaaki Tanino, Yuji Haishima   +12 more
doaj   +2 more sources

Fit for purpose testing and independent GMP validation of the monocyte activation test [PDF]

Current Research in Toxicology
The present study describes the “fit for purpose” testing and the independent product-specific GMP validation of the monocyte activation test (MAT) to detect pyrogenic and pro-inflammatory contaminants, MAT Method A, Quantitative Test (European ...
Ruth Daniels, Wim Van der Elst, Chi K. So, Liesbeth Voeten, Philip Breugelmans, Marijke W.A. Molenaar-de Backer, Stephen Poole, Mehul Patel   +7 more
doaj   +2 more sources

In vitro alternative for reactogenicity assessment of outer membrane vesicle based vaccines [PDF]

Scientific Reports, 2023
Intrinsic or added immune activating molecules are key for most vaccines to provide desired immunity profiles but may increase systemic reactogenicity. Regulatory agencies require rabbit pyrogen testing (RPT) for demonstration of vaccine reactogenicity ...
Marijke W. A. Molenaar-de Backer, Paulien Doodeman, Fereshte Rezai, Lisa M. Verhagen, Arno van der Ark, Els M. Plagmeijer, Bernard Metz, Naomi van Vlies, Olga Ophorst, René H. M. Raeven   +9 more
doaj   +2 more sources

The Effectiveness of Chelating Solutions and Photodynamic Therapy in Inactivating Bacterial Lipopolysaccharides During Endodontic Therapy [PDF]

European Endodontic Journal
Objective: The purpose of this study was to evaluate the influence effects of different endodontic treatments –, such as chelation ng solutions and antimicrobial photodynamic therapy (aPDT), on the rreductionng the amount of endotoxin levels in root ...
Denise Ferracioli Oda, Mirela Cesar Barros, Talita Tartari, Felipe Eduardo de Oliveira Silva, Christiane Becari Ribeiro, Luciane Dias De Oliveira, Flaviana Bombarda De Andrade   +6 more
doaj   +2 more sources

Recirculating glass pipettes constitute a high risk when working with freshly isolated immune cells - the presence of bacterial pyrogenic material [PDF]

Frontiers in Cellular and Infection Microbiology
A number of immune cells are highly sensitive to pyrogenic substances such as bacterial lipopolysaccharides (LPS). It is therefore crucial to ensure that all material coming in contact with these cells are pyrogen free.
Srinivas Akula, Sandra Lara, Anna-Karin Olsson, Lars Hellman   +3 more
doaj   +2 more sources

Advancing Pyrogen Testing for Vaccines with Inherent Pyrogenicity: Development of a Novel Reporter Cell-Based Monocyte Activation Test (MAT) [PDF]

Vaccines
Background/Objectives: Pyrogens, fever-inducing substances from biological or environmental sources, are recognized by Toll-like receptors (TLRs) predominantly expressed by human monocytes and represent a critical quality attribute (CQA) for ...
Sijia Yi, Jenny Xu, Liping Song, Frank Celeste, Christopher J. Wang, Melissa C. Whiteman   +5 more
doaj   +2 more sources

A novel alternative for pyrogen detection based on a transgenic cell line [PDF]

Signal Transduction and Targeted Therapy
Pyrogen, often as a contaminant, is a key indicator affecting the safety of almost all parenteral drugs (including biologicals, chemicals, traditional Chinese medicines and medical devices).
Qing He, Chuan-Fei Yu, Gang Wu, Kai-Qin Wang, Yong-Bo Ni, Xiao Guo, Zhi-Hao Fu, Lan Wang, De-Jiang Tan, Hua Gao, Can Wang, Gang Chen, Xu-Hong Chen, Bo Chen, Jun-Zhi Wang   +14 more
doaj   +2 more sources

Phytochemical screening and antimicrobial activity of Polygala sadebeckiana Gürke extracts on bacterial isolates from Wound samples of patients with “Shimetere” [PDF]

BMC Complementary Medicine and Therapies
Background Modern medicine is not the choice of patients with “shimetere” in the Gurage community owing to their perception of ‘parenteral medication use severely aggravates the disease’.
Bereket Zeleke, Zebene Mekonnen, Meskele Bireda, Melaku Yitbarek, Andamlak Dendir   +4 more
doaj   +2 more sources

Pharmacokinetics and the effectiveness of pyrogen-free bioabsorbable wet adhesives [PDF]

Scientific Reports
Bioabsorbable materials are essential for advanced therapies, including surgical sealing, cell therapy, and drug delivery. Natural bioabsorbable materials, including collagen and hyaluronic acid, have better biocompatibility than synthetic bioabsorbable ...
Risa Oshima, Kumiko Yoshihara, Ko Nakanishi, Tsukasa Akasaka, Shinji Shimoji, Teppei Nakamura, Takumi Okihara, Mariko Nakamura, Akihiro Matsukawa, Ikkei Tamada, Bart Van Meerbeek, Tsutomu Sugaya, Yasuhiro Yoshida   +12 more
doaj   +2 more sources

Biocompatibility analysis of titanium bone wedges coated by antibacterial ceramic-polymer layer [PDF]

Scientific Reports
This paper presents the surface treatment results of titanium, veterinary bone wedges. The functional coating is composed of a porous oxide layer (formed by a plasma electrolytic oxidation process) and a polymer poly(sebacic anhydride) (PSBA) layer ...
Alicja Kazek-Kęsik, Daria Gendosz de Carrillo, Weronika Maciak, Anna Taratuta, Zuzanna Walas, Damian Matak, Wojciech Simka   +6 more
doaj   +2 more sources