Results 31 to 40 of about 138,584 (219)
Analyzing clonal variation of monoclonal antibody-producing CHO cell lines using an in silico metabolomic platform. [PDF]
PLoS ONE, 2014 Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins.
Atefeh Ghorbaniaghdam +3 more
doaj +1 more source
Impaired G2/M cell cycle arrest induces apoptosis in pyruvate carboxylase knockdown MDA-MB-231 cells
Biochemistry and Biophysics Reports, 2021 Background: Previous studies showed that suppression of pyruvate carboxylase (PC) expression in highly invasive breast cancer cell line, MDA-MB-231 inhibits cell growth as a consequence of the impaired cellular biosynthesis. However, the precise cellular
Khanti Rattanapornsompong +6 more
doaj +1 more source
The citrate cleavage pathway and lipogenesis in rat adipose tissue: replenishment of oxaloacetate
Journal of Lipid Research, 1967 Fatty acid synthesis via the citrate cleavage pathway requires the continual replenishment of oxaloacetate within the mitochondria, probably by carboxylation of pyruvate.Malic enzyme, although present in adipose tissue, is completely localized in the ...
F.J. Ballard, Richard W. Hanson
doaj +1 more source
Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells
Nature Communications, 2023 Deregulated oxidative metabolism is a hallmark of leukaemia. While tyrosine kinase inhibitors (TKIs) such as imatinib have increased survival of chronic myeloid leukaemia (CML) patients, they fail to eradicate disease-initiating leukemic stem cells (LSCs)
Kevin M. Rattigan +18 more
doaj +1 more source
Assessment of hepatic pyruvate carboxylase activity using hyperpolarized [1-13C]-L-lactate
Magnetic Resonance in Medicine, 2020 To evaluate the utility of hyperpolarized [1‐13C]‐l‐lactate to detect hepatic pyruvate carboxylase activity in vivo under fed and fasted conditions.
Jun Chen +4 more
semanticscholar +1 more source
Metabolic Engineering Communications, 2015 The effect of pyruvate kinase gene (pyk) deletion on the physiology of Corynebacterium glutamicum ATCC13032 was investigated under biotin-sufficient, non-glutamate-producing conditions.
Kazunori Sawada +5 more
doaj +1 more source
Pyruvate Carboxylase in Rhodopseudomonas spheroides [PDF]
Journal of General Microbiology, 1969 SUMMARY: Unlike some other photosynthetic bacteria, Rhodopseudomonas spheroides directly carboxylates pyruvate in a reaction catalysed by a pyruvate carboxylase (E.C. 6·4.1·1). A partially purified preparation of the enzyme was acetylCoA-dependent. No phosphoenolpyruvate synthetase or phosphoenolpyruvate carboxylase activity was detected in extracts of
J, Payne, J G, Morris
openaire +2 more sources
Frontiers in Physiology, 2019 It is hypothesized that glucokinase (GCK) is the glucose sensor not only for regulation of insulin release by pancreatic β-cells, but also for the rest of the cells that contribute to glucose homeostasis in mammals. This includes other cells in endocrine
Franz M. Matschinsky, David F. Wilson
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2021 Wild-type C. glutamicum ATCC 13032 is known to possess two enzymes with anaplerotic (C4-directed) carboxylation activity, namely phosphoenolpyruvate carboxylase (PEPCx) and pyruvate carboxylase (PCx). On the other hand, C3-directed decarboxylation can be
Jannick Kappelmann +8 more
doaj +1 more source
Stem Cell Research, 2023 Pyruvate carboxylase (PC) deficiency (PCD), due to biallelic PC variants, is a rare inherited metabolic disease, which is characterized by seizures, global developmental delay, as well as lactic acidosis, and elevated plasma pyruvate and alanine levels ...
Jingjing Sun, Pei Lu, Yunlin Shen, Li Ma
doaj +1 more source