Analyzing clonal variation of monoclonal antibody-producing CHO cell lines using an in silico metabolomic platform. [PDF]
PLoS ONE, 2014 Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins.
Atefeh Ghorbaniaghdam +3 more
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Impaired G2/M cell cycle arrest induces apoptosis in pyruvate carboxylase knockdown MDA-MB-231 cells
Biochemistry and Biophysics Reports, 2021 Background: Previous studies showed that suppression of pyruvate carboxylase (PC) expression in highly invasive breast cancer cell line, MDA-MB-231 inhibits cell growth as a consequence of the impaired cellular biosynthesis. However, the precise cellular
Khanti Rattanapornsompong +6 more
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A Substrate-induced Biotin Binding Pocket in the Carboxyltransferase Domain of Pyruvate Carboxylase [PDF]
, 2013 Biotin-dependent enzymes catalyze carboxyl transfer reactions by efficiently coordinating multiple reactions between spatially distinct active sites. Pyruvate carboxylase (PC), a multifunctional biotin-dependent enzyme, catalyzes the bicarbonate- and ...
Lietzan, Adam D, Maurice, Martin St.
core +2 more sources
Phosphoenolpyruvate Carboxykinase as the Sole Anaplerotic Enzyme in Saccharomyces cerevisiae [PDF]
, 2010 Pyruvate carboxylase is the sole anaplerotic enzyme in glucose-grown cultures of wild-type Saccharomyces cerevisiae. Pyruvate carboxylase-negative (Pyc–) S.
Zelle, Rintze M. +5 more
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The citrate cleavage pathway and lipogenesis in rat adipose tissue: replenishment of oxaloacetate
Journal of Lipid Research, 1967 Fatty acid synthesis via the citrate cleavage pathway requires the continual replenishment of oxaloacetate within the mitochondria, probably by carboxylation of pyruvate.Malic enzyme, although present in adipose tissue, is completely localized in the ...
F.J. Ballard, Richard W. Hanson
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Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells
Nature Communications, 2023 Deregulated oxidative metabolism is a hallmark of leukaemia. While tyrosine kinase inhibitors (TKIs) such as imatinib have increased survival of chronic myeloid leukaemia (CML) patients, they fail to eradicate disease-initiating leukemic stem cells (LSCs)
Kevin M. Rattigan +18 more
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Insights Into the Carboxyltransferase Reaction of Pyruvate Carboxylase From the Structures of Bound Product and Intermediate Analogs [PDF]
, 2013 Pyruvate carboxylase (PC) is a biotin-dependent enzyme that catalyzes the MgATP- and bicarbonate-dependent carboxylation of pyruvate to oxaloacetate, an important anaplerotic reaction in central metabolism.
Lietzan, Adam D., Maurice, Martin St.
core +2 more sources
Glucagon promotes increased hepatic mitochondrial oxidation and pyruvate carboxylase flux in humans with fatty liver disease. [PDF]
Cell MetabPetersen KF +3 more
europepmc +2 more sources
Structure and substrate selectivity of the 750-kDa α6β6 holoenzyme of geranyl-CoA carboxylase. [PDF]
, 2015 Geranyl-CoA carboxylase (GCC) is essential for the growth of Pseudomonas organisms with geranic acid as the sole carbon source. GCC has the same domain organization and shares strong sequence conservation with the related biotin-dependent carboxylases 3 ...
Huang, Christine S +4 more
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Metabolic Engineering Communications, 2015 The effect of pyruvate kinase gene (pyk) deletion on the physiology of Corynebacterium glutamicum ATCC13032 was investigated under biotin-sufficient, non-glutamate-producing conditions.
Kazunori Sawada +5 more
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