Results 191 to 200 of about 1,270,348 (335)

Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu, Igor Koop, Gavin Murugan, Thagshanen Thillairajah, Karen M. Polizzi   +4 more
wiley   +1 more source

Impact of Agitation Power Input on Aspergillus oryzae Metabolism: Application of Multi‐Omics on an Industrial Enzyme Fermentation

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The agitation power input substantially impacts the oxygen transfer rate in aerobic fermentation processes. Furthermore, in a carbon‐limited process where the substrate feed rate is controlled by the concentration of dissolved oxygen, the power input will consequently govern the feed rate.
Mariana Albino, Jean‐Marie Mouillon, Gisela Nadal‐Rey, Carina L. Gargalo, Ulrich Krühne, Krist V. Gernaey, Mads O. Albæk   +6 more
wiley   +1 more source

Neonatal Pyruvate Kinase Deficiency Presenting with Severe Hemolytic Anemia and Liver Failure. [PDF]

Children (Basel)
Hsu YH, Jiang CB, Hou JY, Jim WT, Lin SP, Chang SW, Tseng KT, Lee NC.   +7 more
europepmc   +1 more source

Cold atmospheric plasma‐mediated tumor microenvironment remodeling for cancer treatment

BMEMat, EarlyView.
Schematic presentation of CAP‐mediated TME remodeling. This review summarizes recent efforts in cold atmospheric plasma (CAP) application in cancer treatment, highlighting the anticancer potential of CAP, molecular mechanisms, and future perspectives for further improvement and clinical translation.
Israr Khan, Qiujie Fang, Cao Fei, Ziyuan Wang, Zhaowei Chen, Guojun Chen, Zhiming Xu, Shu Xu, Zhitong Chen   +8 more
wiley   +1 more source

Pyruvate kinase deficiency modifies sickle hemoglobin carrier and sickle cell disease phenotypes in mice. [PDF]

JCI Insight
Wang X, Smith M, Kamimura S, Li Q, Shah N, Quezado M, Almeida LE, Vogel S, Tegegn MB, Sun KY, Villasmil R, Liu C, Eaton WA, Thein SL, Quezado ZM.   +14 more
europepmc   +1 more source

Nanomaterials‐mediated glycolysis rewriting to potentiate tumor immunotherapy

BMEMat, EarlyView.
This review systematically summarizes cutting‐edge advances in glycolysis‐rewiring nanomedicines, emphasizing their mechanisms in reversing immunosuppression and reinvigorating antitumor immune responses. Challenges in clinical translation and future directions for designing multifunctional metabolic‐immune modulators are also critically discussed ...
Zilin Ma, Songyan Li, Jie Yan, Xuechun Wang, Wen Zhao, Yunxuan Song, Dongdong Liu, Jianwei Jiao, Xiuying Duan, Guiqiang Zhang   +9 more
wiley   +1 more source

Clinical spectrum of pyruvate kinase deficiency: data from the Pyruvate Kinase Deficiency Natural History Study.

Blood, 2018
R. Grace, P. Bianchi, E. V. van Beers, S. Eber, B. Glader, H. Yaish, J. Despotovic, J. Rothman, Mukta Sharma, Melissa M McNaull, E. Fermo, K. Lezon-Geyda, D. Morton, E. Neufeld, S. Chonat, N. Kollmar, C. Knoll, K. Kuo, J. Kwiatkowski, D. Pospíšilová, Y. Pastore, A. Thompson, P. Newburger, Y. Ravindranath, Winfred C. Wang, M. Wlodarski, Heng Wang, S. Holzhauer, V. Breakey, J. Kunz, S. Sheth, M. Rose, Heather A. Bradeen, Nolan Neu, Dongjing Guo, Hasan Al-Sayegh, W. London, P. Gallagher, A. Zanella, W. Barcellini   +39 more
semanticscholar   +1 more source

Targeting neutrophil extracellular traps in metabolic and immune niche: Nanomaterials for diabetes tissue regeneration

BMEMat, EarlyView.
The effects of NETs on regeneration of various diabetic tissues, and strategies targeting NETs for diabetes tissue regeneration. In the diabetic environment, NETs undergo complex metabolic and immune reprogramming, leading to dynamic changes in antibacterial and proinflammatory functions, and affecting regeneration of multiple systemic tissues.
Xinyi Jiang, Muxin Yue, Kai Mao, Boon Chin Heng, Noor Azlin Yahya, Yunsong Liu, Zheng Li   +6 more
wiley   +1 more source

Immunoassay for pyruvate kinase M1/2 as an Alzheimer's biomarker in CSF. [PDF]

Open Life Sci
de Geus MB, Kivisäkk P, Trombetta BA, Tijms BM, Visser PJ, Arnold SE, Carlyle BC.   +6 more
europepmc   +1 more source

Harnessing ferroptosis from multilayer defense networks to nanoplatforms for specific cancer therapy

BMEMat, EarlyView.
Nanomaterials target metabolically‐regulated ferroptosis for cancer therapy. Iron‐based or alternative nanoplatforms integrate ferroptosis with chemotherapy, immunotherapy, or radiotherapy. They enable stimulus‐responsive therapies (photothermal, photodynamic, sonodynamic) activated by near‐infrared, light, or ultrasound, achieving potent synergistic ...
Xinyue Xu, Xuejian Wang, Wenyan Jiang, Wan Hairul Anuar Kamaruddin, Chunyan Fang, Pengfei Zhang   +5 more
wiley   +1 more source