Results 141 to 150 of about 596,924 (249)

Injectable Dual‐Network Hydrogel System for Osteochondral Repair Combining Immunomodulation, Mechanical Adaptability, and Enhanced Tissue Integration

Advanced Functional Materials, EarlyView.
A UV‐triggered injectable dual‐network hydrogel is reported as the first application of bletilla striata polysaccharide (BSP) in osteochondral repair. By integrating methacrylamide‐modified BSP and nitrobenzaldehyde‐functionalized hyaluronic acid, the system achieves immunomodulation, mechanical reinforcement, and dynamic tissue adhesion, thereby ...
Jiaming Cui, Feifei Zhou, Yuan Luo, Tao Ouyang, Huiwu Zhang, Songmiao Cheng, Qiang Li, Xu Jiang, Yingying Yu, Yicheng Wang, Hong Ming   +10 more
wiley   +1 more source

A duplex real-time fluorescent quantitative PCR method for simultaneous and rapid detection of <i>Actinobacillus pleuropneumoniae</i> and influenza A virus. [PDF]

Front Vet Sci
Ren YJ, Lin H, Yu DJ, Zhang J, Yang F, Zhang BY, Chen YQ, Long HX, Ren LL.   +8 more
europepmc   +1 more source

VIVID: A qPCR‐Based Platform for Sensitive and Quantitative In Vivo Tracking of Extracellular Vesicles

Advanced Functional Materials, EarlyView.
This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian, Talin Ebrahimian, Jonathan Miranda O'Connor, Janusz Rak, Stephanie Lehoux, Maryam Tabrizian   +5 more
wiley   +1 more source

Evaluation of practices for the diagnosis and monitoring of cytomegalovirus infection in kidney transplant recipients in Brazil. [PDF]

J Bras Nefrol
Hatem NA, Keitel E, Santoro-Lopes G, Pierrotti LC, Pasqualotto AC.   +4 more
europepmc   +1 more source

Nano‐ and Micro‐Sized Solid Materials Used as Antiviral Agents

Advanced Functional Materials, EarlyView.
Due to the rise of viral infections in humans and possible viral outbreaks, the use of nano‐ or micro‐sized materials as antiviral agents is rapidly increasing. This review explores their antiviral properties against RNA and DNA viruses, either as a prevention or a treatment tool, by delving into their mechanisms of action and how to properly assess ...
Orfeas‐Evangelos Plastiras, Imelda Raczkiewicz, Peggy Bouquet, Jean‐Philippe Dacquin, Jeremy Dhainaut, Anne Goffard, Christophe Volkringer   +6 more
wiley   +1 more source

Development of a novel duplex crystal digital PCR for the detection of PRRSV-1 and PRRSV-2. [PDF]

Front Cell Infect Microbiol
Shi Y, He J, Shi K, Yin Y, Long F, Feng S, Wei Z.   +6 more
europepmc   +1 more source

qPCR: Technical Replicate Variation

BioTechniques, 2011
This month's question from the Molecular Biology Forums (online at molecularbiology. forums.biotechniques.com) comes from the “Real-Time qPCR/qRT-PCR Methods” section. Entries have been edited for concision and clarity. Mentions of specific products and manufacturers have been retained from the original posts, but do not represent endorsements by, or ...
openaire   +1 more source

Mechanoregulatory Effects of Cell‐Scale Microwells on Epithelial Cell Phenotype

Advanced Functional Materials, EarlyView.
In small polycaprolactone microwells, A549 epithelial cells span well edges, in contrast to cells growing on flat substrates. Focal adhesion sites (yellow) concentrate at topographic boundaries, while cytoskeletal tension (magenta stress fibers) is transmitted to the nucleus (blue), reducing nuclear sphericity.
Ruiwen He, Wang Sik Lee, Phattadon Yajan, Aaron Lee, Mauro Sousa de Almeida, Sandeep Keshavan, Jozef Adamcik, Céline Loussert‐Fonta, Dimitri Vanhecke, Barbara Rothen‐Rutishauser, Alke Petri‐Fink   +10 more
wiley   +1 more source

Correction: Development and partial validation of an RT-qPCR assay for the rapid detection of spring viremia of carp virus (SVCV). [PDF]

Front Microbiol
Frontiers Production Office.
europepmc   +1 more source

Biointerface Membranes Orchestrating Site‐Specific Osteoimmunomodulatory and Antibacterial Effects for Enhanced Osseous Regeneration in Periodontal Therapy

Advanced Functional Materials, EarlyView.
Here, a biointerface membrane engineered with site‐specific interfacial properties is developed. During implantation between gingival and bone defect, the membrane creates a pro‐osteogenic microenvironment, precisely modulates cellular activities at each biointerface, and facilitates the orchestration of complex healing events, ultimately leading to ...
Yuwei Zhu, Tingting Wang, Jing Zhu, Bingtian Zhao, Bingyang Dai, Xiaoyue Zhang, Wei Liu, Cheng Yang, Cheng Dong, Ren Liu, Lei Zhao, Jun Wang, Peilei Shi, To Ngai   +13 more
wiley   +1 more source