Results 161 to 170 of about 1,004,764 (341)

qPCR (Thermo Fisher) v1

, 2019
The expression levels of 15 genes identified as DGE were validated by RT-qPCR. The RNA samples used for the qRT-PCR assays were the same as those for the RNA-Seq experiments. The cDNA synthesis and qRT-qPCR reactions were performed using the SuperScript® III First-Strand Synthesis System (Thermo Fisher) and the Power SYBR® Green RT-PCR Reagents Kit ...
Rosangela Bevitori, Sanchari Sicar, Raquel Neves de Mello, Roberto Coiti Togawa, Tiago Silva Oliveira, Maria de Fátima Grossi-de-Sá, Nita Parekh¶   +6 more
openaire   +1 more source

The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time.

Trends in Biotechnology, 2019
Sean C. Taylor, Katia Nadeau, Meysam Abbasi, C. Lachance, Marie Nguyen, Joshua Fenrich   +5 more
semanticscholar   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

qPCR: Single Copy Targets [PDF]

BioTechniques, 2013
openaire   +3 more sources

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source

Mathematical modeling based on RT-qPCR analysis of SARS-CoV-2 in wastewater as a tool for epidemiology [PDF]

, 2021
Naďa Krivoňáková, Andrea Šoltýsová, Michal Tamáš, Zdenko Takáč, Ján Krahulec, Andrej Ficek, Miroslav Gál, Marián Gall, Miroslav Fehér, Anna Krivjanská, Ivana Horáková, Noemi Belišová, Paula Bímová, Andrea Škulcová, Tomáš Mackuľak   +14 more
openalex   +1 more source

DNA extraction leads to bias in bacterial quantification by qPCR

Applied Microbiology and Biotechnology, 2022
Ângela Lima, Ângela França, C. Muzny, Christopher M. Taylor, N. Cerca   +4 more
semanticscholar   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

NR4A1 Exerts Pro‐Tumor Role in Glioblastoma via Inducing xCT/GPX4‐Regulated Ferroptosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Purpose This study investigates NR4A1's paradoxical roles in glioblastoma (GBM) progression, focusing on its mechanistic link to ferroptosis regulation. We aimed to resolve conflicting reports of NR4A1 as both an oncogene and a tumor suppressor by defining its transcriptional control over xCT/GPX4‐mediated iron homeostasis and its clinical ...
Peng Tao, Shikuan Din, Zhengkang Fu, Qian Sun, Xiwei Zhu, Yuxin Wei, Huan Qu, Xinyi Zhang, Jiaxuan Liu, Chuhua Fu, Qianxue Chen   +10 more
wiley   +1 more source