Results 291 to 300 of about 1,617,447 (322)

Connexin 26 Functions as a Direct Transcriptional Regulator During the Cochlea Development

Advanced Science, EarlyView.
Connexin26 can not only form intercellular channels that mediate rapid communication on the cell membrane, but also enter the nucleus as a transcription factor to directly regulate the transcription of nuclear genes. In the developing cochlea, Cx26 can control the maturation of the molecular scissor ADAM10 by regulating the transcription of TspanC8 ...
Xiaozhou Liu, Le Xie, Yuan Jin, Sen Chen, Xinyu Shi, Yanjun Zong, Zhengdong Zhao, Wei‐Jia Kong, Yu Sun   +8 more
wiley   +1 more source

Comparative Analysis of Gene Expression at Sea Level and High Altitude: A Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Approach. [PDF]

Cureus
Alharthi SB, Alsubai AH, Almalki SK, El-Shehawi AM, Eldebsy AM, Alsoliman AA, Alharthi RF, Morsi M, Alharthi D, Mutabaqani RA.   +9 more
europepmc   +1 more source

Quantitative Proteomics and CRISPR/Cas9 Editing Reveal UPR‐Mediated Control of Immunoglobulin Homeostasis in Hybridomas

Advanced Science, EarlyView.
BCR sequencing and subclone analysis correlated immunoglobulin (Ig) chain loss in dysfunctional hybridomas with disrupted monoclonal antibody homeostasis. Proteomics‐guided CRISPR/Cas9 editing revealed that the unfolded protein response (UPR) regulates aberrant Ig synthesis.
Rubing Zou, Xinying Lu, Ying Liu, Peiyu Yang, Shuo Sun, Yihua Liu, Yinyuan Mo, Guonian Zhu, Jae Seong Lee, Yirong Guo   +9 more
wiley   +1 more source

Assessment of reference genes for qRT-PCR normalization to elucidate host response to African swine fever infection. [PDF]

Braz J Microbiol
Rajkhowa S, Sonowal J, Sengar GS, Pegu SR, Deb R, Das PJ, Doley J, Paul S, Gupta VK.   +8 more
europepmc   +1 more source

PDIA3 Inhibition Facilitates Sensitivity of IKE‐Induced Ferroptosis via STAT3/LCN2 Axis to Improve Glioblastoma Therapy

Advanced Science, EarlyView.
In this manuscript, protein disulfide isomerase A3 (PDIA3) is identified as a key factor mediating the susceptibility of ferroptosis in GBM. Inhibition of PDIA3 enhances IKE or cystine starvation‐induced ferroptosis in GBM cells by resulting in the accumulation of lipid peroxidation and a reduction in GSH level.
Jie Zhang, Wei Wang, Xin Liu, Peifen Lu, Xinjing Liu, Qiucheng Nie, Siyuan Xin, Hong Li, Donglin Yu, Xinyue Zhang, Kailong Li, Xiaomin Han, Shuping Zhang, Wei Chong, Lili Sun, Wei Li, Tao Xin, Jin Jiao, Qiang Ma, Yiju Wei   +19 more
wiley   +1 more source

Evaluation and Validation of Reference Genes for Gene Expression Analysis Using qRT-PCR in the Sugarcane Stem Borer Chilo sacchariphagus (Lepidoptera: Pyralidae). [PDF]

Insects
Wang Z, Shang X, Wei J, Tian X, Liu Y, Zhang G.   +5 more
europepmc   +1 more source

Selection and Validation of qRT-PCR Internal Reference Genes to Study Flower Color Formation in Camellia impressinervis. [PDF]

Int J Mol Sci
Zhang P, Chen S, Chen S, Zhu Y, Lin Y, Xu X, Liu Z, Zou S.   +7 more
europepmc   +1 more source

Identification and Validation of Reference Genes for qRT-PCR Analysis of Petal-Color-Related Genes in Rosa praelucens. [PDF]

Genes (Basel)
Jian H, Wang H, Qiu X, Yan H, Ma L.
europepmc   +1 more source

Development of a quadruple qRT-PCR assay for simultaneous identification of hypervirulent and carbapenem-resistant Klebsiella pneumoniae. [PDF]

Microbiol Spectr
Xu Z, Li B, Jiang Y, Huang J, Su L, Wu W, Pang Q, Li Z, Zhang J, Li X, Wang J, Cen F, Peng L, Liang J, Wang F, Liu C, Shen C, Liu Y, Yang Y.   +18 more
europepmc   +1 more source

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Stem-Loop qRT-PCR-Based Quantification of miRNAs.

Methods in molecular biology, 2021
Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR.
Y. Abel, M. Rederstorff
semanticscholar   +3 more sources