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Stem-Loop qRT-PCR for the Detection of Plant microRNAs.
Methods in molecular biology, 2017Plant microRNAs (miRNAs) play important roles in the posttranscriptional regulation of protein-coding genes, and they are essential for a normal development and survival. Mature miRNAs are cleaved from larger precursor RNAs and are typically 21-22 nt long.The small size, the lack of a common feature like a poly(A) tail, 3' end-modifications, and ...
E. Varkonyi-Gasic
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Cold Spring Harbor Protocols, 2008
INTRODUCTIONBacteria growing in biofilms often express a different subset of genes compared to the same strains growing planktonically. Quantitative reverse transcriptase real time PCR (qRT-PCR) can be used effectively to quantify the number of RNA transcripts of specific genes from bacteria growing in biofilms.
Ailyn C, Pérez-Osorio +1 more
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INTRODUCTIONBacteria growing in biofilms often express a different subset of genes compared to the same strains growing planktonically. Quantitative reverse transcriptase real time PCR (qRT-PCR) can be used effectively to quantify the number of RNA transcripts of specific genes from bacteria growing in biofilms.
Ailyn C, Pérez-Osorio +1 more
openaire +2 more sources
2010
Plant small RNAs are a class of 19- to 25-nucleotide (nt) RNA molecules that are essential for genome stability, development and differentiation, disease, cellular communication, signaling, and adaptive responses to biotic and abiotic stress. Small RNAs comprise two major RNA classes, short interfering RNAs (siRNAs) and microRNAs (miRNAs).
Varkonyi-Gasic, Erika, Hellens, Roger
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Plant small RNAs are a class of 19- to 25-nucleotide (nt) RNA molecules that are essential for genome stability, development and differentiation, disease, cellular communication, signaling, and adaptive responses to biotic and abiotic stress. Small RNAs comprise two major RNA classes, short interfering RNAs (siRNAs) and microRNAs (miRNAs).
Varkonyi-Gasic, Erika, Hellens, Roger
openaire +3 more sources
Gene Amplification and qRT-PCR
2014This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell
Cerith, Jones, Alain, Filloux
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Continuous-flow, microfluidic, qRT-PCR system for RNA virus detection
Analytical and Bioanalytical Chemistry, 2017One of the main challenges in the diagnosis of infectious diseases is the need for rapid and accurate detection of the causative pathogen in any setting. Rapid diagnosis is key to avoiding the spread of the disease, to allow proper clinical decisions to be made in terms of patient treatment, and to mitigate the rise of drug-resistant pathogens.
B Leticia, Fernández-Carballo +7 more
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RSV Growth and Quantification by Microtitration and qRT-PCR Assays
2016Defective interfering viral particles have been reported as important determinants of the course of viral infection, and they can markedly temper the virulence of the infection. Here, we describe a simple method, based on limiting dilution, for the removal of defective interfering particles from RSV.
Hayat, Caidi +2 more
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Quantification of NMDAR Subunit Genes Expression by qRT-PCR
2017Transcription is the initial and generally the most sensitive step to cellular needs and environmental cues. Thus, it serves as a major mechanism controlling gene expression. Using reverse-transcription quantitative polymerase chain reaction technology (RT-qPCR), we will present how to quantify the transcriptional expression of NMDARs subunits during ...
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Laser Capture Microdissection of Archival Kidney Tissue for qRT-PCR
2015Whole-organ molecular analysis of the kidney potentially misses important factors involved in the pathogenesis of disease in glomeruli and tubules. Organ wide analysis can however be augmented by using laser capture microdissection (LCM) to isolate morphologically similar cells and nephron structures from a heterogeneous tissue section via direct ...
Tim D, Hewitson +2 more
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RNA integrity and the effect on the real-time qRT-PCR performance.
Molecular Aspects of Medicine, 2006S. Fleige, M. Pfaffl
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