Results 31 to 40 of about 383,746 (345)

Real-time PCR for mRNA quantitation.

BioTechniques, 2005
Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to ...
M. Wong, J. Medrano
semanticscholar   +1 more source

Qualitative and Quantitative Analysis Method of Recombinant Collagen in Complex Matrix Based on HPLC-MS/MS

Separations
The purpose of this study is to achieve the quantitative detection of recombinant type III collagen (rh-COL-III) in dressings with complex matrix. First of all, the marker peptide (GEAGIPGVPGAK) of rhCOL-III was identified with HPLC-MS/MS.
Jianping Gao, Liping Jia, Zhenhu Guo, Liming Xu, Daidi Fan, Guifeng Zhang   +5 more
doaj   +1 more source

Quantitative Determination of Four Potential Genotoxic Impurities in the Active Pharmaceutical Ingredients in TSD-1 Using UPLC-MS/MS

Molecules, 2022
A novel method of ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for the identification and quantification of four potential genotoxic impurities (PGIs) in the active pharmaceutical ingredients of TSD-1, a ...
Taiyu Wang, Hailong Yang, Jie Yang, Ningjie Guo, Guodong Wu, Xueyu Xu, Ming An   +6 more
doaj   +1 more source

Absolute quantitation of microbiota abundance in environmental samples

Microbiome, 2018
Microbial communities (microbiota) influence human and animal disease and immunity, geochemical nutrient cycling and plant productivity. Specific groups, including bacteria, archaea, eukaryotes or fungi, are amplified by PCR to assess the relative ...
Andrzej Tkacz, M. Hortala, P. Poole
semanticscholar   +1 more source

Improved intact peptide and protein quantitation by LC‐MS: Battling the deleterious effects of analyte adsorption

Analytical Science Advances, 2021
Peptide and protein quantitation by liquid chromatography‐mass spectrometry relies on the assumption of linear signal response with concentration. At low concentrations, analyte adsorption to pipette tips, sample vials and equipment can have significant ...
EmmaRae L. Murphy, Andrew P. Joy, Rodney J. Ouellette, David A. Barnett   +3 more
doaj   +1 more source

Standardization of SARS-CoV-2 Cycle Threshold Values: Multisite Investigation Evaluating Viral Quantitation across Multiple Commercial COVID-19 Detection Platforms

Microbiology Spectrum, 2023
The demand for testing during the coronavirus disease 2019 (COVID-19) pandemic has resulted in the production of several different commercial platforms and laboratory-developed assays for the detection of severe acute respiratory syndrome coronavirus 2 ...
Kenneth Gavina, Lauren C. Franco, Christopher M. Robinson, Weston Hymas, Guang-Sheng Lei, Will Sinclair, Tara Hall, John Carlquist, John-Paul Lavik, Christopher L. Emery, Phillip R. Heaton, David Hillyard, Bert K. Lopransi, Ryan F. Relich   +13 more
doaj   +1 more source

Harmonized procedures lead to comparable quantification of total oxylipins across laboratories

Journal of Lipid Research, 2020
Oxylipins are potent lipid mediators involved in a variety of physiological processes. Their profiling has the potential to provide a wealth of information regarding human health and disease and is a promising technology for translation into clinical ...
Malwina Mainka, Céline Dalle, Mélanie Pétéra, Jessica Dalloux-Chioccioli, Nadja Kampschulte, Annika I. Ostermann, Michael Rothe, Justine Bertrand-Michel, John W. Newman, Cécile Gladine, Nils Helge Schebb   +10 more
doaj   +1 more source

Phytochemical profiling of the stem bark of Betula utilis from different geographical regions of India using UHPLC‐ESI‐MS/MS

Analytical Science Advances, 2021
A simple, rapid, sensitive, and reliable ultra‐high‐performance liquid chromatography‐hybrid linear ion trap triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 10 bioactive compounds in stem bark of ...
Tripti Mishra, Preeti Chandra, Brijesh Kumar, Meena Baleshwar, Pushpa Joshi, Tikam Singh Rana, Dalip Kumar Upreti, Mahesh Pal   +7 more
doaj   +1 more source

Rapid measurement of serum caffeine concentrations in acuteclinical settings

Toxicology Communications, 2021
The number of acute caffeine poisoning cases have increased in Japan. We can use serum caffeine concentrations to evaluate the severity of caffeine poisoning and determine whether or not we should perform hemodialysis. In this study, we sought to develop
Tomoki Hanazawa, Yoshito Kamijo, Tomohiro Yoshizawa, Kiyotaka Usui   +3 more
doaj   +1 more source

Gas-liquid chromatographic analysis of free long-chain aldehydes

Journal of Lipid Research, 1969
Quantitative results are reported for gas-liquid chromatography of mixtures of free tetra-, hexa-, and octadecanals and cis-9-octadecenal on polar and nonpolar liquid phases.
Randall Wood, R.D. Harlow
doaj   +1 more source