Results 241 to 250 of about 1,295,509 (307)

Significant difference in gut microbiota Bifidobacterium species but not Lactobacillus species in colorectal cancer patients in comparison with healthy volunteers using quantitative real-time PCR. [PDF]

PLoS One
Esfandiari F, Bakhshi B, Shahbazi T, Derakhshan-Nezhad E, Bahroudi M, Minaeeian S, Boustanshenas M, Alborzi F, Behboudi B, Fazeli MS.   +9 more
europepmc   +1 more source

Identification and selection of reference genes for analysis of gene expression by quantitative real-time PCR in the euhalophyte Suaeda altissima (L.) Pall. [PDF]

Commun Integr Biol
Khramov DE, Nedelyaeva OI, Konoshenkova AO, Volkov VS, Balnokin YV.   +4 more
europepmc   +1 more source

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Basic principles of real-time quantitative PCR

Expert Review of Molecular Diagnostics, 2005
Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications.
Manit, Arya, Iqbal S, Shergill, Magali, Williamson, Lyndon, Gommersall, Neehar, Arya, Hitendra R H, Patel   +5 more
openaire   +4 more sources

Quantitative Real-Time PCR: Recent Advances.

Methods in molecular biology, 2016
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or ...
C. Singh, S. Roy-Chowdhuri
semanticscholar   +3 more sources

Accurate Real-time Reverse Transcription Quantitative PCR

Methods in Molecular Biology, 2009
Within the last few years real-time quantitative PCR has become the method of choice for the accurate quantification of mRNA levels. Compared to previous methods the sensitivity of real-time quantitative PCR improved to the detection limit of up to one single molecule per reaction tube.
Marco, Klatte, Petra, Bauer
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Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.

Neuroscience Letters, 2003
C. Ramakers, J. Ruijter, R. Lekanne Deprez, A. Moorman   +3 more
semanticscholar   +3 more sources

Design of primers and probes for quantitative real-time PCR methods.

Methods in Molecular Biology, 2015
Alicia Rodríguez, Mar Rodríguez, J. Córdoba, M. Andrade   +3 more
semanticscholar   +3 more sources

Novel One-Step Single-Tube Nested Quantitative Real-Time PCR Assay for Highly Sensitive Detection of SARS-CoV-2

Analytical Chemistry, 2020
Coronavirus disease 2019 (COVID-19) has become a public health emergency. The reverse transcriptase real-time quantitative PCR (qRT-PCR) test is currently considered as the gold standard in the laboratory for the etiological detection of COVID-19 ...
Ji Wang, Kun Cai, Rui-Qing Zhang, Xiao-Zhou He, Xin-Xin Shen, Jun Liu, Junqiang Xu, Feng Qiu, Wen-Wen Lei, Jin-Rong Wang, Xin-Na Li, Yuan Gao, Yong-Zhong Jiang, Wen-Bo Xu, Xue-Jun Ma   +14 more
semanticscholar   +1 more source

Real-time quantitative PCR in parasitology

Trends in Parasitology, 2002
Standard techniques for counting parasites are often time-consuming, difficult and inaccurate, and occasionally unpleasant. Real-time quantitative polymerase chain reaction has recently been applied to parasitology, specifically Plasmodium, Toxoplasma, Leishmania and Neospora.
Andrew, Bell, Lisa, Ranford-Cartwright
openaire   +3 more sources

Quantitative detection of periodontopathogens by real-time PCR

Journal of Microbiological Methods, 2004
Specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. We report here the development of a sensitive, quantitative PCR technique for enumerating Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg ...
Claudia, Nonnenmacher, Alexander, Dalpke, Reinier, Mutters, Klaus, Heeg   +3 more
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