Results 261 to 270 of about 1,295,509 (307)

Quantitative (Real-Time) RT-PCR in Cardiovascular Research

2007
Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples.
Ashton, KJ, Headrick, JP
openaire   +2 more sources

Real-Time Quantitative PCR, Pathogen Detection and MIQE

2012
Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination
Gemma, Johnson, Tania, Nolan, Stephen A, Bustin   +2 more
openaire   +2 more sources

Validation of a quantitative method for real time PCR kinetics

Biochemical and Biophysical Research Communications, 2002
Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which the quantitative methods are based. The generally accepted mathematical model assumes that amplification efficiencies are equal at the exponential phase of the reactions for the same amplicon ...
Liu, W., Saint, D.
openaire   +3 more sources

Real-Time Quantitative PCR Analysis of Viral Transcription

2004
Whole-genome profiling using DNA arrays has led to tremendous advances in our understanding of cell biology. It has had similar success when applied to large viral genomes, such as the herpesviruses. Unfortunately, most DNA arrays still require specialized and expensive resources and, generally, large amounts of input RNA. An alternative approach is to
James, Papin, Wolfgang, Vahrson, Rebecca, Hines-Boykin, Dirk P, Dittmer   +3 more
openaire   +2 more sources

Semi-Quantitative Real-Time PCR for Pain Research

2004
In this chapter we cover what we have found to be a "best practice" for real-time polymerase chain reaction (PCR) for relative mRNA quantification. We describe our techniques for tissue-sample collection and freezing, sample handling for quick and reproducible extraction of total RNA, first strand cDNA synthesis, real-time PCR amplification, and ...
Hong Qing, Guo, Sandra R, Chaplan
openaire   +2 more sources

Multiplex Analyses Using Real-Time Quantitative PCR

2016
Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene expression in real time. Multiplex qPCR requires the use of probe-based assays, in which each probe is labeled with a unique fluorescent dye, resulting in different observed colors for each assay.
Steve F C, Hawkins, Paul C, Guest
openaire   +2 more sources

Guideline to reference gene selection for quantitative real-time PCR.

Biochemical and Biophysical Research Communications - BBRC, 2004
A. Radonic, S. Thulke, I. Mackay, O. Landt, W. Siegert, A. Nitsche   +5 more
semanticscholar   +1 more source

Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR.

Biochemical and Biophysical Research Communications - BBRC, 2006
Mukesh K. Jain, Aashima Nijhawan, A. Tyagi, J. Khurana   +3 more
semanticscholar   +1 more source

Analyzing real-time PCR data by the comparative CT method

Nature Protocols, 2008
Thomas D. Schmittgen, K. Livak
semanticscholar   +1 more source

Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing.

Journal of Microbiological Methods, 2006
Wen-Bin Li, J. Hartung, L. Levy
semanticscholar   +1 more source