Results 251 to 260 of about 1,295,509 (307)

Accuracy of quantitative real-time PCR analysis

Doklady Biochemistry and Biophysics, 2013
When solving many problems in biology and med icine, it is necessary to detect the presence of specific nucleic acid sequences in samples. For this purpose, polymerase chain reaction (PCR)—a chain enzy matic reaction of multiplication of the analyzed DNA fragment, realized in the form of successive rounds of duplication of fragments present in the ...
D G, Sochivko, A A, Fedorov, D A, Varlamov, V E, Kurochkin, R V, Petrov   +4 more
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Evaluation of uncertainty in quantitative real-time PCR

Journal of Microbiological Methods, 2006
Quantitative real-time PCR is one of the newer methods for measurement of the amount of nucleic material in biological systems. However, reliable measurement requires an appropriate estimation of uncertainty and this paper has developed the uncertainty budget associated with this procedure using as an example, data from a quantitative real-time PCR ...
John L, Love, Paula, Scholes, Brent, Gilpin, Marion, Savill, Susan, Lin, Laly, Samuel   +5 more
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Real-Time Quantitative PCR of Telomere Length

Molecular Biotechnology, 2004
Telomeres cap the ends of chromosomes and are essential for the protection of chromosomes, as well as restricting the replicative potential of a cell. These functions are achieved by the regulation of telomeric repeat length, making the measurement of telomere length a useful aid in the elucidation of the replicative history and potential of cells ...
Marcel E, Gil, Thérèsa L, Coetzer
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High‐Throughput Quantitative Real‐Time PCR

Current Protocols in Human Genetics, 2008
AbstractRecent technical advances in quantitative real‐time PCR (qRT‐PCR) have allowed for extensive miniaturization, thereby rendering the technique amenable to high‐throughput assays. Large numbers of different nucleic acids can now rapidly be measured quantitatively. Many investigations can benefit from this approach, including determination of gene
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Quantitative Real-Time PCR in aDNA Research

2011
Quantitative real-time PCR (qPCR) is a technique that is widely used in the field of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing.
Bunce, Michael, Oskam, C., Allentoft, M.
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Quantitative Real-Time RT-PCR

2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA, NARDON, ERMANNO, PRACELLA, DANAE, BONIN, Serena   +3 more
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Quantitative measurement of streptomycetes using real-time PCR

Journal of Environmental Monitoring, 2006
A quantitative real-time PCR method was developed and used for determination of streptomycetes in indoor dust samples of five homes collected during three years. The specificity of the method was tested with 14 Streptomyces and ten non-streptomycetous species, revealing a high specificity for mesophilic streptomycetes. Thermophilic species and S. albus
Helena, Rintala, Aino, Nevalainen
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Real-Time Quantitative PCR for the Detection of Fetal Aneuploidies

2008
In prenatal analysis, one of the major concerns is the detection of fetal aneuploidies. Several molecular methods have been described recently for the rapid analysis of amniotic fluid and chorionic villi. Fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) of short tandem repeats are already implemented in prenatal ...
Bernhard G, Zimmermann, Lech, Dudarewicz
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RNA Isolation and Real-Time Quantitative RT-PCR

2008
Adipose tissue has emerged as a major endocrine organ producing a wide spectrum of hormones and factors that play crucial roles in regulating cell turnover and function, not only locally within the adipose tissue but also in the brain and other key metabolic organ systems.
Haiyan, Guan, Kaiping, Yang
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Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
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