Results 71 to 80 of about 281,649 (291)
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
Effects of quenching and partial quenching on penguin matrix elements [PDF]
Journal of High Energy Physics, 2001 In the calculation of non-leptonic weak decay rates, a "mismatch" arises when the QCD evolution of the relevant weak hamiltonian down to hadronic scales is performed in unquenched QCD, but the hadronic matrix elements are then computed in (partially) quenched lattice QCD.
Golterman, Maarten, Pallante, Elisabetta
openaire +3 more sources
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
A SERS quenching method for the sensitive determination of insulin
, 2020 In this work, we utilise the disulphide bond structure of insulin and a new benzothiazole Raman probe for the detection of human insulin using surface‐enhanced Raman spectroscopy (SERS).
Mahnaz D. Gholami +7 more
core +1 more source
Fluorescence-based methods to study rapid dynamics and conformational flexibility in peptides [PDF]
, 2004 Intramolecular collision of polypeptides is the primary step in protein folding, the dynamics of which is of importance for understanding this fascinating topic.
Huang, Fang
core +1 more source
Thermo-Microstructural Numerical Simulation of Quenching Process of Steels
Journal of Advanced Materials in Engineering, 2011 In this work, a 3D thermo-microstructural model was developed to simulate the continuous cooling of steel. The model was employed for simulation of cooling process of the gears made from a plain carbon steel (AISI 1045) and a low alloy steel (AISI 4140).
M. Eshraghi Kakhki +2 more
doaj
Error Propagation Analysis for Quantitative Intracellular Metabolomics
Metabolites, 2012 Model-based analyses have become an integral part of modern metabolic engineering and systems biology in order to gain knowledge about complex and not directly observable cellular processes. For quantitative analyses, not only experimental data, but also
Jana Tillack +4 more
doaj +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source