Results 41 to 50 of about 78,155 (186)

The TRAPP subunit Trs130p interacts with the GAP Gyp6p to mediate Ypt6p dynamics at the late Golgi

Frontiers in Cell and Developmental Biology, 2016
Small GTPases of the Rab superfamily participate in virtually all vesicle-mediated trafficking events. Cycling between an active GTP-bound form and an inactive GDP-bound form is accomplished in conjunction with guanine nucleotide exchange factors (GEFs ...
Stephanie eBrunet, Djenann eSaint-Dic, Miroslav P. Milev, Tommy eNilsson, MICHAEL eSACHER, MICHAEL eSACHER   +5 more
doaj   +1 more source

Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. [PDF]

PLoS ONE, 2011
The Rab family of Ras-related GTPases are part of a complex signaling circuitry in eukaryotic cells, yet we understand little about the mechanisms that underlie Rab protein participation in such signal transduction networks, or how these networks are ...
Christopher D Heger, Christiane D Wrann, Ruth N Collins   +2 more
doaj   +1 more source

Rep15 interacts with several Rab GTPases and has a distinct fold for a Rab effector

Nature Communications, 2022
Rep15 is an effector that interacts with the GTPase Rab15. Here, the authors show that Rep15 also interacts with Rab3 paralogs and Rab34, present crystal structures of Rep15:Rab complexes and find that Rep15 depletion in glioblastoma cells decreases ...
Amrita Rai, Anurag K. Singh, Nathalie Bleimling, Guido Posern, Ingrid R. Vetter, Roger S. Goody   +5 more
doaj   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Rab cycling in Arabidopsis chloroplasts.

, 2013
Rab GTPase in its inactive, Rab-GDP, form is transformed into its active, Rab-GTP, form by an unidentified GEF in the stroma. A GAP promotes activity of the Rab GTPase to perform this action.
Emelie Lindquist (396890), Nadir Zaman Khan (396889), Henrik Aronsson (396891)   +2 more
core   +1 more source

Anterograde Trafficking of CXCR4 and CCR2 Receptors in a Prostate Cancer Cell Line

Cellular Physiology and Biochemistry, 2013
Background: Most prostate cancer-related deaths result from metastasis. CXCR4 and CCR2 are known to govern cellular processes resulting in cell migration, proliferation and survival.
Kelsie Gillies, Jaime Wertman, Nicholle Charette, Denis J. Dupré   +3 more
doaj   +1 more source

GBS‐MeDIP: A method for the simultaneous identification of genetic and epigenetic variants in tumors

FEBS Open Bio, EarlyView.
GBS‐MeDIP combines DNA sequencing with enrichment of methylated DNA to profile genetic and epigenetic variation in the same reduced genomic fraction. Applied to gastric cancer and parathyroid tumors, it identifies sequence variants, copy number changes, and differentially methylated regions together, helping distinguish true epigenetic changes from ...
Fábio Pértille, Shiva Rezaei, Julia Uffenorde, Oliver Gimm, Mohammad Ali Hosseinpour Feizi, Luiz Lehmann Coutinho, Carlos Guerrero‐Bosagna   +6 more
wiley   +1 more source

Rab1A Promotes Hepatic Steatosis by Suppressing Mitophagy via the Raf‐1/ERK1/2/PINK1 Signaling Axis

Advanced Science, EarlyView.
Why does fat accumulate in the liver? Our study reveals Rab1A as the molecular switch that silences the cell's mitochondrial cleanup crew. Turning Rab1A off reactivates mitophagy, clears excess fat, and reverses fatty liver disease in mice—offering a promising new therapeutic target for MASLD, a growing global health challenge.
Li Zhang, Jianhua Li, Huilu Zhang, Qi Qin, Yichen Huang, Xiaofan Tian, Chao Sun, Binbin Li, Zhengxin Wang, Xin Zhang   +9 more
wiley   +1 more source

TBC proteins: GAPs for mammalian small GTPase Rab?

, 2011
The TBC (Tre-2/Bub2/Cdc16) domain was originally identified as a conserved domain among the tre-2 oncogene product and the yeast cell cycle regulators Bub2 and Cdc16, and it is now widely recognized as a conserved protein motif that consists of approx ...
Mitsunori Fukuda
core   +1 more source