Results 121 to 130 of about 5,224 (170)
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Immunochemical quantitation of antigens by single radial immunodiffusion
Immunochemistry, 1965Abstract When an unknown amount of antigen is allowed to diffuse radially from a well in a uniformly thin layer of antibody-containing agar for a sufficient time to allow all antigen to combine, the final area reached by the precipitate is directly proportional to the amount of antigen employed, and inversely proportional to the concentration of ...
G, Mancini +2 more
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Quantitation of lens proteins by radial immunodiffusion method
Experimental Eye Research, 1973Abstract The quantitation of α- and γ-crystallin in ten different bovine lenses has been determined by radial immunodiffusion method.
P D, Mehta, S, Lerman
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Antigen quantitation by a hemolytic radial immunodiffusion assay
Immunochemistry, 1978Abstract The radial immunoprecipitin assay for antigen quantitation (Mancini's assay) was modified into a radial immunohemolytic assay, whereby the antigen lyses erythrocytes coated with the complementary antibody. Thus, when rabbit immunoglobulin G (IgG) diffused in Petri dishes from wells into agar gels containing erythrocytes coated with purified ...
F J, Demma +4 more
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A Staining Rack for Radial Immunodiffusion Plates
American Journal of Clinical Pathology, 1970A readily constructed rack is described tha permits the uniform handling of as many as 10 radial immunodiffusion plates simultaneously during antibody elution and subsequent staining. The device consists of a circular baseplate with a centrally located, vertical axial rod and a series of hollow cylindrical spacers that thread onto the rod.
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2018
Radial immunodiffusion (RID) is a quantitative test, and is often used in serology laboratories to quantify the concentration of a certain antigen or antibody class in a patient’s serum. The technique is very simple and cost-effective; however, it may require a long incubation time; hence, some laboratories prefer to use alternative methods such as an ...
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Radial immunodiffusion (RID) is a quantitative test, and is often used in serology laboratories to quantify the concentration of a certain antigen or antibody class in a patient’s serum. The technique is very simple and cost-effective; however, it may require a long incubation time; hence, some laboratories prefer to use alternative methods such as an ...
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An evaluation of a method of quantitative radial immunodiffusion
Molecular Immunology, 1968Abstract The single radial diffusion method has been studied experimentally and theoretically. The results demonstrate that the diffusion of antigen through an agarose sheet impregnated with antibody can be described by a two-dimensional diffusion equation which contains an expression to compensate for reaction between antigen and antibody.
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Construction and use of a template block for radial immunodiffusion
Analytical Biochemistry, 1990Details for design and construction of template blocks for the increased sensitivity of radial immunodiffusion (RID) assays are described. The sensitivity of the template RID was 10-fold higher for quantifying albumin and 5-fold higher for IgG than the conventional RID with wells cut into the agarose. The system described in this communication resulted
T L, McDonald, A, Weber, G, Thiele
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1996
Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1). In this method, the antigen--antibody precipitation is made more sensitive than in double immunodiffusion (see Chapter 135) by the incorporation of the antiserum in the agar solution before the gel is made (2). Thus, the antiserum is uniformly distributed
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Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1). In this method, the antigen--antibody precipitation is made more sensitive than in double immunodiffusion (see Chapter 135) by the incorporation of the antiserum in the agar solution before the gel is made (2). Thus, the antiserum is uniformly distributed
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Quantitative Radial Immunodiffusion
1979Mancini et al. (1965) developed a very simple method to quantitate antigens using gel diffusion. The principle is this: The antigen is placed in a well punched into an agar layer. The agar was mixed while melted at 45°-50°C with the corresponding antibody.
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[20] Single radial immunodiffusion
1981Publisher Summary This chapter describes the different aspects of single radial immunodiffusion (SRID). SRID is an immunodiffusion technique, in which a single partner of the antigen–antibody reaction, usually the antigen (Ag), diffuses radially from a small well punched into a gel layer of constant thickness and the other partner, usually the ...
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