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Principles of protein estimation by radial immunodiffusion. [PDF]

Journal of Clinical Pathology, 1971
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Influence of merthiolate on single radial immunodiffusion

Klinische Wochenschrift, 1972
In the course of quantitative immunoglobulin determinations by radial immunodiffusion systematical differences in the results have been observed. These differences possibly are due to an influence of merthiolate (sodium aethyl mercurithiosalicylat) which is added to the agar to avoid bacterial growth. We proved this possibility by experiments under the
O Wetter, Wetter O
exaly   +3 more sources

Quantitative radial immunodiffusion assay for serum amyloid A protein

Journal of Immunological Methods, 1983
A radial immunodiffusion assay for serum amyloid A protein (SAA) using a commercially available antiserum is described. Serum is applied untreated to 1% agarose gels prepared in 0.02 M barbitone buffer, pH 8.6, containing 40 g/l polyethylene glycol 6000. Incubation is carried out overnight at 37 degrees C.
Robin E Chambers, John T Whicher, J T Whicher   +2 more
exaly   +3 more sources

Radial Immunodiffusion (RID)

, 2018
Radial immunodiffusion (RID) is a quantitative test, and is often used in serology laboratories to quantify the concentration of a certain antigen or antibody class in a patient’s serum. The technique is very simple and cost-effective; however, it may require a long incubation time; hence, some laboratories prefer to use alternative methods such as an ...
Rowa Yousef Alhabbab
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Single Radial Immunodiffusion

, 1996
Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1). In this method, the antigen--antibody precipitation is made more sensitive than in double immunodiffusion (see Chapter 135) by the incorporation of the antiserum in the agar solution before the gel is made (2). Thus, the antiserum is uniformly distributed
Graham S. Bailey
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Quantitative Radial Immunodiffusion

, 1979
Mancini et al. (1965) developed a very simple method to quantitate antigens using gel diffusion. The principle is this: The antigen is placed in a well punched into an agar layer. The agar was mixed while melted at 45°-50°C with the corresponding antibody.
Alois Nowotny
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[20] Single radial immunodiffusion

, 1981
Publisher Summary This chapter describes the different aspects of single radial immunodiffusion (SRID). SRID is an immunodiffusion technique, in which a single partner of the antigen–antibody reaction, usually the antigen (Ag), diffuses radially from a small well punched into a gel layer of constant thickness and the other partner, usually the ...
Jean-Pierre Vaerman
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Radial Immunodiffusion

, 2011
Jan W. Gooch
core   +4 more sources

A METHOD FOR INCREASING THE SENSITIVITY OF RADIAL-IMMUNODIFFUSION ASSAY

Lancet, The, 1970
Elliot Alpert
exaly   +3 more sources

Re-evaluation of the haptoglobin reference values with the radial immunodiffusion technique

Clinical Biochemistry, 1984
The reference values of the three main types of serum haptoglobin Hp 1-1, Hp 2-1, and Hp 2-2, as determined by radial immunodiffusion and with phenotype determination on polyacrylamide gel electrophoresis have been re-evaluated for both sexes.
Jaap Schrijver, Wil H P Schreurs
exaly   +1 more source