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An evaluation of a method of quantitative radial immunodiffusion
Molecular Immunology, 1968Abstract The single radial diffusion method has been studied experimentally and theoretically. The results demonstrate that the diffusion of antigen through an agarose sheet impregnated with antibody can be described by a two-dimensional diffusion equation which contains an expression to compensate for reaction between antigen and antibody.
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Construction and use of a template block for radial immunodiffusion
Analytical Biochemistry, 1990Details for design and construction of template blocks for the increased sensitivity of radial immunodiffusion (RID) assays are described. The sensitivity of the template RID was 10-fold higher for quantifying albumin and 5-fold higher for IgG than the conventional RID with wells cut into the agarose. The system described in this communication resulted
T L, McDonald, A, Weber, G, Thiele
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An intrinsig inaccuracy of radial immunodiffusion measurements of incomplete antigens
Clinical Biochemistry, 1970Summary If an antigen lacks some of the kinds of determinant groups characteristic of its species then calculation of its concentration from ring size measurements on radial immunodiffusion can lead to errors. Optical densities of the rings can also be used to determine concentrations.
A, Lietze, C, Sinclair, A H, Rowe
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Improvements to the enzyme-developed radial immunodiffusion technique
Journal of Immunological Methods, 2002An enzyme-developed radial immunodiffusion technique, previously known as the diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA), has been improved in two ways: (a) antibody-containing spots have been made larger and more distinct by revealing them with a mixture of hydrogen peroxide, 3,3'-diaminobenzidine and nickel, and further ...
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Radial immunodiffusion and rocket Immunoelectrophoresis
1979Specific protein concentrations may be measured in gel media by two techniques: single radial immunodiffusion and rocket Immunoelectrophoresis. The former method is called ‘single’ to distinguish it from the qualitative Ouchter-lony or ‘double diffusion’ methods, and ‘radial’ to contrast it with the earlier ‘linear diffusion’ technique of Oudin1 ...
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Detecting Microalbuminuria by Radial Immunodiffusion
Diabetic Medicine, 1985D, Cheţa, G, Stoica
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Quantitation of bovine immunoglobulins by radial immunodiffusion
Veterinary Record, 1979openaire +2 more sources
Quantitation of antigen in radial immunodiffusion plates
Clinica Chimica Acta, 1969openaire +2 more sources