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Radioimmunoassay of Glibenclamide

Diabetes, 1979
A specific, sensitive, and simple radioimmunoassay was developed for the oral hypoglycemic drug glibenclamide, N-4-[2-(5-chloro-2-methoxy-benzamide)- ethyl]-benzenesulfonyl-N'-cyclohexylurea. Antiserum against glibenclamide was obtained from rabbits immunized with an antigen prepared by conjugating the diazonium salt of N-(p-amino-benzamidoethyl ...
Ayako Matsuda   +2 more
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Radioimmunoassay of ligandin

Gastroenterologia Japonica, 1979
A sensitive and specific radioimmunoassay for rat and human serum ligandin was developed using antisera generated in rabbits by the injection of ligandin purified from liver. Because rat ligandin and human ligandin have no cross reactivity, radioimmunoassay systems for each of the serum ligandins were prepared. Highly purified ligandin labeled with125I
Masanobu Tsuru, S. Murao
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A radioimmunoassay for diphenylhydantoin

Clinica Chimica Acta, 1973
Abstract A radioimmunoassay for the anticonvulsant compound diphenylhydantoin (DPH) has been developed. Antisera were prepared by immunizing animals with a DPH-chicken γ-globulin conjugate. The assay depends on the capacity of unlabelled DPH to compete with a known amount of [14C]DPH for the antibody-binding sites. The assay can detect as little as 0.
Richard L. Rapport   +3 more
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Radioimmunoassay for immunoconglutinins

Journal of Immunological Methods, 1980
A competitive radioimmunoassay for immunoconglutinin (IK) was developed using isolated antostimulated rabbit IK labelled with 125I and glutaraldehyde-fixed sheep erythrocytes coated with fixed complement (EAC). IKs in test sera of human or animal origin produced specific inhibition of uptake of [125I]IK onto EAC and this was quantitated by reference to
C. André   +3 more
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Radioimmunoassay

2003
Radioimmunoassay is often described in terms of the competition between a radiolabeled antigen (Ag*) and its unlabeled counterpart (Ag) for binding to a limited amount of specific antibody (Ab) (1). In most radioimmunoassays the reaction is allowed to proceed to equilibrium and thus, can be represented by Eq. (1). [Formula: see text].
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Radioimmunoassay

Angewandte Chemie International Edition in English, 1976
AbstractBiologically active substances are often effective in nanogram or even smaller quantities. Investigations of their mode of action therefore require highly sensitive microanalytical methods of detection. Chemical, chromatographic or spectrometric methods frequently lack the required sensitivity; it was only after the discovery of the ...
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The radioimmunoassay of buprenorphine

European Journal of Clinical Pharmacology, 1980
Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay have good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of ...
M. R. D. Bennett   +6 more
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