Results 161 to 170 of about 2,283,209 (217)
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GPCR-Radioligand Binding Assays
Methods in Cell Biology, 2016Radioligand binding assays provide sensitive and quantitative information about guanine nucleotide protein G protein-coupled receptor (GPCR) expression and affinity for a wide variety of ligands, making them essential for drug structure-activity studies and basic GPCR research.
Colleen A Flanagan
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Simple Radioligand Binding Assay for the Determination of Urinary Scopolamine
Journal of Pharmaceutical Sciences, 1984A sensitive radioligand binding assay is described for the determination of scopolamine in human urine. As a measure for the drug concentration, the quantitative displacement of scopolamine of tritiated quinuclidinyl benzylate from rat brain receptors was used.
Helmut Bittiger
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Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors
Methods in Molecular Biology, 2016In receptor-ligand interactions, dissociation constants provide a key parameter for characterizing binding. Here, we describe filter-based radioligand binding assays at equilibrium, either varying ligand concentrations up to receptor saturation or outcompeting ligand from its receptor with increasing concentrations of ligand analogue.
Luz Irina A. Calderón Villalobos +1 more
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Dopamine D4 Receptor in Human Peripheral Blood Lymphocytes: A Radioligand Binding Assay Study
Neuroscience Letters, 1997 The expression of dopamine D4 receptor was investigated in human peripheral blood lymphocytes with a radioligand binding assay technique, using [3H]clozapine as radioligand. [3H]Clozapine was specifically bound to human peripheral blood lymphocytes.
Amenta Francesco +2 more
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Journal of Pharmacology and Experimental Therapeutics, 1999 Interaction with the exsorptive transporter P-glycoprotein (P-gp) is a possible source of peculiarities in drug pharmacokinetics, including dose-dependent absorption, drug-drug interactions, intestinal secretion, and limited permeability of the blood ...
Constanze Hilgendorf +2 more
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A radioligand-binding assay for adenosine in tissue extracts
Analytical Biochemistry, 1978Abstract A radioligand-binding assay employing adenine analog-binding protein from rabbit crythrocytes and [ 3 H]adenosine of high specific activity measures less than 1 pmol of adenosine in neutral HClO 4 extracts of tissue. Adenine and its nucleotides interfere with adenosine binding, so are removed by preliminary chromatography on PEI and ...
R A, Olsson +3 more
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In Vitro Muscarinic Receptor Radioligand‐Binding Assays
Current Protocols in Pharmacology, 2010AbstractG‐protein‐coupled muscarinic receptors (mAChRs), of which there are five subtypes (M1‐M5), are attractive drug targets for a number of disorders. Described in this unit are radioligand‐binding assays for defining the selectivity and affinity of chemical agents at the five mAChR subtypes. Detailed methodologies and troubleshooting strategies are
Tod, Steinfeld +3 more
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2003
Characterization and cloning of the AT(1) and AT(2) receptors would not have been possible without an assay that could detect and measure the density and affinity of these receptors. The most frequently used, if not the only, methodological approach used to investigate these receptors is the radioligand binding assay performed either in a test tube ...
G, Thibault, E, L Schiffrin
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Characterization and cloning of the AT(1) and AT(2) receptors would not have been possible without an assay that could detect and measure the density and affinity of these receptors. The most frequently used, if not the only, methodological approach used to investigate these receptors is the radioligand binding assay performed either in a test tube ...
G, Thibault, E, L Schiffrin
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Radioligand Binding Assays and Their Analysis
2012Radioligand binding is widely used to characterize receptors and determine their anatomical distribution, particularly the superfamily of seven transmembrane-spanning G protein-coupled receptors for both established transmitters such as endothelin-1 and an increasing number of orphan receptors recently paired with their cognate ligands.
Janet J, Maguire +2 more
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BIOLOGICAL RECEPTORS: IMPLICATIONS OF RADIOLIGAND QUALITY IN BINDING ASSAYS
Journal of Biological Systems, 2002We tested the ability of different fitting procedures to discern differences in the binding affinity between the labeled and unlabeled ligand in a receptor assay, in order to obtain the correct value of the radiotracer specific activity. This point is critical for the correct conversion of the estimated binding constants to meaningful units.
Venturino, Andrés, Rivera, Elena S.
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