Results 171 to 180 of about 2,283,209 (217)

Microplate gel-filtration method for radioligand-binding assays

Analytical Biochemistry, 2002
A thin-layer gel-filtration chromatographic method has been developed in a 96-well format to separate free and protein-bound ligand in radioligand-binding assays. The mobile phase in the gel-filtration plate is removed via quick centrifugation before samples are applied.
Jianwei, Liu, Anna, Zacco, Timothy M, Piser, Clay W, Scott   +3 more
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Optimization of Radioligand Binding and Radioimmuno Assays

Analytical Biochemistry, 1996
Radioligand binding assay (RLA) and radioimmunoassay (RIA) development involves consideration of a large number variables which influence signal readout or system errors, with contrary effects on assay performance. We have developed an optimization strategy according to statistical criteria which incorporates these variables and which specifies a ...
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A Method for Radioligand Binding Assays Using a Robotic Workstation

Journal of Receptor Research, 1993
Radioligand binding assays provide a powerful tool for screening drug candidates at many receptors. We present a method utilizing a robotic workstation, the Biomek 1000, which automates the tedious and repetitive tasks of these assays. First, the robot handles the serial dilution of up to 8 drugs with 11 concentrations per drug. The sequential addition
B, Cusack, E, Richelson
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Radioligand Binding Assays: Theory and Practice

1996
Transmitters and hormones generate signals in target cells by interacting with a signal transducing protein or receptor. Receptors may be located in the cytosol, allowing for example, steroid and thyroid hormones to control gene transcription. However, the largest family comprises G-protein linked seven transmembrane spanning receptors located in the ...
Anthony P. Davenport, Fraser D. Russell
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Radioligand Binding Assays for Adenosine Receptors

1990
The technique of radioligand binding is one that has been of considerable importance in defining receptor function at the molecular level. In addition to providing evidence for the potential existence of receptor subtypes, subclasses, and states (Williams and Enna, 1986; Williams and Sills, 1989), the ease and resource efficiency of the technique makes
Michael Williams, Kenneth A. Jacobson
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A Sensitive Radioligand Binding Assay for Timolol in Plasma

Journal of Pharmaceutical Sciences, 1991
A sensitive and reproducible radioreceptor assay (RRA) is described for the determination of low picogram levels of timolol in plasma. The plasma or serum samples (1 mL) are prepared by selectively extracting timolol with lipophilic solvents or, alternatively, only plasma (serum) proteins are precipitated prior to binding assay. The recovery of timolol
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Radioligand Binding Assays

1984
Early work aimed at examining the properties of chemical transmission focused on presynaptic events such as neurotransmitter synthesis, degradation, accumulation and release. In contrast, because of technical limitations, less information was obtained in regard to the biochemical events associated with neurotransmitter receptors.
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A radioligand binding assay to measure anti-thyroperoxidase autoantibodies in mice

Journal of Immunological Methods, 2007
Autoimmune (Hashimoto's) thyroiditis is a chronic inflammatory disease which affects >3% of the population and shows an increasing prevalence with increasing age. Anti-thyroid autoantibodies, particularly against thyroperoxidase (also known as thyroid peroxidase or TPO), occur commonly in humans with autoimmune thyroid disease, and assays for anti-TPO ...
Sarah L, Hayward, Kunimasa, Suzuki, John F, Elliott   +2 more
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Determination of radioligand specific activity using competition binding assays

Analytical Biochemistry, 1992
Radioligand binding assays are routinely utilized in laboratories throughout the world to study receptors and their related binding sites, carrier proteins, and enzymes. To accurately estimate equilibrium binding parameters, such as the equilibrium dissociation constant (Kd) and maximal number of binding sites (Bmax), the investigator must know the ...
H L, Wiener, M E, Reith
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Binding of Amitriptyline to Adenosine A1 or A2a Receptors Using Radioligand Binding Assay

International Journal of Pharmacology, 2017
Background and Objective: Tricyclic antidepressants such as amitriptyline (AMT) may result in life-threatening cardiovascular toxicities. Previous studies showed that AMT-induced cardiovascular toxic effects were prevented/reversed by selective adenosine receptor (AR) antagonists.
Kalkan, Şule, Hocaoğlu Aksay, Nil, Gürdal, Hakan, Buyukdeligoz, Mujgan   +3 more
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