Results 181 to 190 of about 2,283,209 (217)
Some of the next articles are maybe not open access.
Radioligand Binding Assays: Application of [125I]Angiotensin II Receptor Binding
2009Angiotensin II (AngII) is an octapeptide hormone with a key role in blood pressure regulation. AngII increases blood pressure by stimulating G protein-coupled receptors in vascular smooth muscle. AngII receptors are therefore an important target in patients with high blood pressure. Strategies to lower high blood pressure (hypertension) include the use
Wayne R, Leifert +3 more
openaire +2 more sources
Radioligand-binding assays for study of neurotensin receptors
1989Publisher Summary This chapter discusses the radioligand-binding assays for the study of neurotensin receptors. Radioligand-binding studies have provided valuable information concerning the characteristics, the structure–activity relationships, and the cellular localization of neurotensin receptors.
openaire +2 more sources
Magnetic molecularly imprinted polymer beads for drug radioligand binding assay
The Analyst, 1998Molecularly imprinted polymer-magnetic iron oxide composite materials which exhibit recognition properties and can be withdrawn from solution by application of a magnetic field were prepared for the first time. Magnetic iron oxide was incorporated using a suspension polymerisation methodology with a perfluorocarbon liquid as the dispersing phase for ...
R J, Ansell, K, Mosbach
openaire +2 more sources
Saturation Assays of Radioligand Binding to Receptors and Their Allosteric Modulatory Sites
Current Protocols in Neuroscience, 1997AbstractThe protocols in this unit describe methods for measuring radioligand binding to sites on the GABA‐A receptor complex. This system was chosen because of the fundamental role of GABA‐A receptors in central nervous system function and the presence of multiple binding sites on one supramolecular complex. However, these basic techniques can be used
openaire +2 more sources
Development of radioligand binding assays for REV-ERBα and REV-ERBβ
Analytical BiochemistryREV-ERBα and REV-ERBβ are atypical nuclear receptors that function as ligand-dependent repressors of transcription. They play critical roles in the regulation of the circadian rhythm, inflammation, and metabolism. The natural ligand for the REV-ERBs is heme, and synthetic ligands (both agonists and antagonists) have been designed and utilized to probe ...
Thomas Koelblen +2 more
openaire +2 more sources
Journal of Bioinformatics and Computational Biology, 2020
As a rule, receptor-ligand assay data are fitted by logistic functions (4PL model, 5PL model, Feldman’s model). The preparation of the initial estimates for parameters of these functions is an important problem for processing receptor-ligand interaction data.
openaire +2 more sources
As a rule, receptor-ligand assay data are fitted by logistic functions (4PL model, 5PL model, Feldman’s model). The preparation of the initial estimates for parameters of these functions is an important problem for processing receptor-ligand interaction data.
openaire +2 more sources
Tissue slices in radioligand binding assays: Studies in brain, pineal and muscle
Life Sciences, 1986The use of tissue homogenates in receptor binding assays raises serious questions as to the physiological value of a preparation which examines receptors (binding sites) in disrupted tissue. In order to usefully study the regulatory properties of neurotransmitter receptors under physiological conditions, the necessity for tissue preparations which ...
M, Wilkinson +2 more
openaire +2 more sources
Identification of drug receptors in porcine dental pulp by the radioligand binding assay
General Pharmacology: The Vascular System, 19891. The acetylcholine (ACh), histamine and serotonin (5-HT) receptors in porcine dental pulp were characterized by the radioligand binding assay. 2. For [3H]nicotine binding site, Kd was 8.06 +/- 1.65 nM and Bmax was 270.83 +/- 32.68 fmol/mg protein. 3. For [3H]QNB binding site, Kd was 1.04 +/- 0.14 nM and Bmax was 24.83 +/- 3.09 fmol/mg protein. 4. For
M, Sano +3 more
openaire +2 more sources
[Radioligand binding assay of PHSA receptor on hepatocytes].
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao, 1994The radioligand binding assay of PHSA receptor on hepatocytes of 7 adults is reported. The presence of PHSA receptor was demonstrated in all the 7 adults, though the individual difference of the Kds and binding capacities among them was distinct with a higher standard derivation.
D, Lei, M, Cai, P, Feng
openaire +1 more source
Multiplexing G protein-coupled receptors in microarrays: A radioligand-binding assay
Analytical Biochemistry, 2007Multiplexing of G protein-coupled receptors (GPCRs) in microarrays promises to increase the efficiency, reduce the costs, and improve the quality of high-throughput assays. However, this technology is still nascent and has not yet achieved the status of "high throughput" or laid claim to handling a large set of receptors.
Bruce, Posner +11 more
openaire +2 more sources