Results 71 to 80 of about 4,191,327 (298)

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Design of trials for interrupting the transmission of endemic pathogens [PDF]

, 2016
Background: Many interventions against infectious diseases have geographically diffuse effects. This leads to contamination between arms in cluster-randomized trials (CRTs).
Silkey, Mariabeth, Tobias Homan, Homan, Tobias, Alexandra Hiscox, Mariabeth Silkey, Thomas A. Smith, Takken, Willem, Nicolas Maire, Maire, Nicolas, Richard Mukabana, Smith, Thomas A., Mukabana, Richard, Hiscox, Alexandra, Willem Takken   +13 more
core   +1 more source

Factors affecting patient participation in orthopaedic trials comparing surgery to non-surgical interventions

Contemporary Clinical Trials Communications, 2016
Background: Randomized controlled trials (RCTs) are considered the most robust research design to determine the effectiveness of interventions. RCTs comparing surgery to non-surgical alternatives are particularly difficult to perform, partly due to ...
Rajat Mittal, Ian A. Harris, Sam Adie, Justine M. Naylor   +3 more
doaj   +1 more source

Cluster‐randomized controlled trials: A tutorial

Cochrane Evidence Synthesis and Methods, 2023
This tutorial focuses on cluster-randomised controlled trials. We will explain what cluster-RCTs are, why they might be used, and how to include data from cluster-randomised controlled trials in systematic reviews. Accompanying the tutorial is a micro-learning module, which will give you the chance to practice adjusting data from cluster-randomised ...
Marty Chaplin, Kerry Dwan
openaire   +3 more sources

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

The challenges faced in the design, conduct and analysis of surgical randomised controlled trials [PDF]

, 2009
Randomised evaluations of surgical interventions are rare; some interventions have been widely adopted without rigorous evaluation. Unlike other medical areas, the randomised controlled trial (RCT) design has not become the default study design for the
Jonathan A Cook, Cook, Jonathan Alistair, Cook, JA, Cook Jonathan A   +3 more
core   +2 more sources

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

When there will never be a randomized controlled trial [PDF]

Kidney International, 2015
There are many unanswered questions about how to manage secondary hyperparathyroidism and associated alterations in mineral metabolism in patients with end-stage renal disease. We provide commentary on an observational study by Komaba et al.
Scialla, Julia J., Wolf, Myles
openaire   +2 more sources

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source