Results 1 to 10 of about 6,495 (196)

Motorless transport of microtubules along tubulin, RanGTP, and salt gradients [PDF]

Nature Communications
Microtubules are dynamic filaments that assemble spindles for eukaryotic cell division. As the concentration profiles of soluble tubulin and regulatory proteins are non-uniform during spindle assembly, we asked if diffusiophoresis - motion of particles ...
Suin Shim, Bernardo Gouveia, Beatrice Ramm, Venecia A. Valdez, Sabine Petry, Howard A. Stone   +5 more
doaj   +5 more sources

Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner [PDF]

eLife, 2015
Soluble karyopherins of the importin-β (impβ) family use RanGTP to transport cargos directionally through the nuclear pore complex (NPC). Whether impβ or RanGTP regulate the permeability of the NPC itself has been unknown.
Alan R Lowe, Jeffrey H Tang, Jaime Yassif, Michael Graf, William YC Huang, Jay T Groves, Karsten Weis, Jan T Liphardt   +7 more
doaj   +3 more sources

XPO5 promotes primary miRNA processing independently of RanGTP [PDF]

Nature Communications, 2020
XPO5 mediates nuclear export of miRNA hairpin precursors (pre-miRNAs) through a RanGTP-dependent binding. Here the authors employ HITS-CLIP and biochemical analysis and show that XPO5 binds and promotes nuclear processing of clustered pri-miRNAs, with ...
Jingjing Wang, Jerome E. Lee, Kent Riemondy, Yang Yu, Steven M. Marquez, Eric C. Lai, Rui Yi   +6 more
doaj   +3 more sources

Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone [PDF]

eLife, 2019
We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface.
Abhilash Padavannil, Prithwijit Sarkar, Seung Joong Kim, Tolga Cagatay, Jenny Jiou, Chad A Brautigam, Diana R Tomchick, Andrej Sali, Sheena D'Arcy, Yuh Min Chook   +9 more
doaj   +3 more sources

Crosstalk between the actin cytoskeleton and Ran-mediated nuclear transport [PDF]

BMC Cell Biology, 2005
Background Transport of macromolecules into and out of the nucleus is a highly regulated process. The RanGTP/RanGDP gradient controls the trafficking of molecules exceeding the diffusion limit of the nuclear pore across the nuclear envelope.
Steward Ruth, Druzhinina Marina, Myers Ron, Minakhina Svetlana   +3 more
doaj   +2 more sources

A multivalent adaptor mechanism drives the nuclear import of proteasomes [PDF]

Nature Communications
Nuclear protein homeostasis, including transcription factor turnover, critically depends on the nuclear proteasomes that must be imported after cell division.
Hanna L. Brunner, Robert W. Kalis, Lorenz Grundmann, Zuzana Hodáková, Zuzana Koskova, Irina Grishkovskaya, Melanie de Almeida, Matthias Hinterndorfer, Hannah Knaudt, Simon Höfflin, Florian Andersch, Harald Kotisch, Achim Dickmanns, Sara Cuylen-Haering, Johannes Zuber, David Haselbach   +15 more
doaj   +2 more sources

Yeast 26S proteasome nuclear import is coupled to nucleus-specific degradation of the karyopherin adaptor protein Sts1 [PDF]

Scientific Reports
In eukaryotes, the ubiquitin–proteasome system is an essential pathway for protein degradation and cellular homeostasis. 26S proteasomes concentrate in the nucleus of budding yeast Saccharomyces cerevisiae due to the essential import adaptor protein Sts1
Carolyn Allain Breckel, Zane M. Johnson, Christopher M. Hickey, Mark Hochstrasser   +3 more
doaj   +2 more sources

CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation. [PDF]

PLoS Biology
The densely packed centromeric heterochromatin at minor and major satellites is comprised of H3K9me2/3 histones, the heterochromatin protein HP1α, and histone variants.
Gokul Gopinathan, Qian Xu, Xianghong Luan, Thomas G H Diekwisch   +3 more
doaj   +2 more sources

HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2 [PDF]

Nature Communications
In vertebrate spindles, most microtubules are formed via branching microtubule nucleation, whereby microtubules nucleate along the side of pre-existing microtubules.
Venecia Alexandria Valdez, Meisheng Ma, Bernardo Gouveia, Rui Zhang, Sabine Petry   +4 more
doaj   +2 more sources

C9orf72 polyPR directly binds to various nuclear transport components [PDF]

eLife
The disruption of nucleocytoplasmic transport (NCT) is an important mechanism in neurodegenerative diseases. In the case of C9orf72-ALS, trafficking of macromolecules through the nuclear pore complex (NPC) might get frustrated by the binding of C9orf72 ...
Hamidreza Jafarinia, Erik van der Giessen, Patrick R Onck   +2 more
doaj   +2 more sources