Results 21 to 30 of about 6,495 (196)
Microtubule Nucleation in Mitosis by a RanGTP-Dependent Protein Complex [PDF]
Current Biology, 2015 The γ-tubulin ring complex (γTuRC) is a multisubunit complex responsible for microtubule (MT) nucleation in eukaryotic cells. During mitosis, its spatial and temporal regulation promotes MT nucleation through different pathways. One of them is triggered around the chromosomes by RanGTP. Chromosomal MTs are essential for functional spindle assembly, but
Scrofani, Jacopo +3 more
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Self-organization of anastral spindles by synergy of dynamic instability, autocatalytic microtubule production, and a spatial signaling gradient. [PDF]
PLoS ONE, 2007 Assembly of the mitotic spindle is a classic example of macromolecular self-organization. During spindle assembly, microtubules (MTs) accumulate around chromatin.
Thomas Clausen, Katharina Ribbeck
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Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals
eLife, 2019 Ran binding protein 1 (RanBP1) is a cytoplasmic-enriched and nuclear-cytoplasmic shuttling protein, playing important roles in nuclear transport. Much of what we know about RanBP1 is learned from fungi. Intrigued by the long-standing paradox of harboring
Yuling Li +9 more
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Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. [PDF]
PLoS ONE, 2008 Formation of female gametes requires acentriolar spindle assembly during meiosis. Mitotic spindles organize from centrosomes and via local activation of the RanGTPase on chromosomes.
Stéphane Brunet +7 more
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The RanGTP gradient – a GPS for the mitotic spindle [PDF]
Journal of Cell Science, 2008 The GTPase Ran has a key role in nuclear import and export, mitotic spindle assembly and nuclear envelope formation. The cycling of Ran between its GTP- and GDP-bound forms is catalyzed by the chromatin-bound guanine nucleotide exchange factor RCC1 and the cytoplasmic Ran GTPase-activating protein RanGAP.
Petr, Kalab, Rebecca, Heald
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Nucleus, 2020 Xenopus egg extracts are a powerful in vitro tool for studying complex biological processes, including nuclear reconstitution, nuclear membrane and pore assembly, and spindle assembly.
Matthew S. Nord +5 more
doaj +1 more source
eLife, 2020 In the nucleus, the spatiotemporal regulation of the catalytic subunit of cAMP-dependent protein kinase A (PKA-C) is orchestrated by an intrinsically disordered protein kinase inhibitor, PKI, which recruits the CRM1/RanGTP nuclear exporting complex.
Cristina Olivieri +13 more
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Higher nucleoporin-Importinβ affinity at the nuclear basket increases nucleocytoplasmic import. [PDF]
, 2013 Several in vitro studies have shown the presence of an affinity gradient in nuclear pore complex proteins for the import receptor Importinβ, at least partially contributing to nucleocytoplasmic transport, while others have historically argued against the
Azimi, Mohammad, Mofrad, Mohammad
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A non-canonical mechanism for Crm1-export cargo complex assembly
eLife, 2015 The transport receptor Crm1 mediates the export of diverse cargos containing leucine-rich nuclear export signals (NESs) through complex formation with RanGTP.
Ute Fischer +6 more
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The F-actin filament capping protein CapG is a bona fide nucleolar protein [PDF]
, 2008 Actin works in concert with myosin I to regulate the transcription of ribosomal genes in the nucleolus. Recently, nucleolar actin has been shown to be active in its polymeric form raising the question how actin dynamics is regulated in the nucleolus ...
Gettemans, Jan +3 more
core +2 more sources