Results 31 to 40 of about 3,517 (188)

Histone Nuclear Import and Beyond: Multifunctional Roles of Importins. [PDF]

Bioessays
Efficient nuclear import of histones requires coordinated action between histone chaperones and nuclear import receptors. Importin‐4, Importin‐9, and Importin‐β/7 not only transport specific histone complexes but also act as chaperones, stabilizing histones and ensuring their protected delivery for nucleosome assembly, highlighting importins as central
Bernardes N, Chook YM.
europepmc   +2 more sources

Targeting the nuclear export receptor exportin-1 in acute myeloid leukaemia: From biology to clinical translation. [PDF]

Clin Transl Med
• XPO1 hyperactivation promotes leukaemogenesis by altering nucleocytoplasmic transport and transcriptional control in acute myeloid leukaemia (AML). • Selinexor and eltanexor show preferential activity in NPM1‐mutated, DEK::NUP214‐positive and SF3B1‐mutated myeloid neoplasms.
Liu Y, Yun X, Ding W, Li S, Liu H.
europepmc   +2 more sources

The RanGTP gradient – a GPS for the mitotic spindle [PDF]

Journal of Cell Science, 2008
The GTPase Ran has a key role in nuclear import and export, mitotic spindle assembly and nuclear envelope formation. The cycling of Ran between its GTP- and GDP-bound forms is catalyzed by the chromatin-bound guanine nucleotide exchange factor RCC1 and the cytoplasmic Ran GTPase-activating protein RanGAP.
Petr, Kalab, Rebecca, Heald
openaire   +2 more sources

Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex

Proceedings of the National Academy of Sciences, 2022
Abstract Previously we showed that the nuclear import receptor Importin-9 wraps around the H2A-H2B core to chaperone and transport it from the cytoplasm to the nucleus. However, unlike most nuclear import systems where RanGTP dissociates cargoes from their importins, RanGTP binds stably to the Importin-9•H2A-H2B complex and formation of
Jenny Jiou, Joy M. Shaffer, Natalia E. Bernades, Ho Yee Joyce Fung, Juliana Kikumoto Dias, Sheena D’Arcy, Yuh Min Chook   +6 more
openaire   +2 more sources

Separable roles for RanGTP in nuclear and ciliary trafficking of a kinesin-2 subunit [PDF]

Journal of Biological Chemistry, 2021
Kinesin is part of the microtubule-binding motor protein superfamily, which serves important roles in cell division and intraorganellar transport. The heterotrimeric kinesin-2, consisting of the heterodimeric motor subunits, kinesin family member 3A/3B (KIF3A/3B), and kinesin-associated protein 3 (KAP3), is highly conserved across species from the ...
Shengping Huang, Larissa L. Dougherty, Prachee Avasthi   +2 more
openaire   +2 more sources

ISWI is a RanGTP-dependent MAP required for chromosome segregation [PDF]

Journal of Cell Biology, 2009
Production of RanGTP around chromosomes induces spindle assembly by activating nuclear localization signal (NLS)–containing factors. Here, we show that the NLS protein ISWI, a known chromatin-remodeling ATPase, is a RanGTP-dependent microtubule (MT)-associated protein. Recombinant ISWI induces MT nucleation, stabilization, and bundling in vitro.
Yokoyama, Hideki, Rybina, Sofia, Santarella-Mellwig, Rachel, Mattaj, Iain W., Karsenti, Eric   +4 more
openaire   +2 more sources

CDK9 interacts with a RanGTP-importin-β complex to regulate erythroid enucleation. [PDF]

J Cell Sci
ABSTRACT Erythroid enucleation is the final stage of erythroid terminal differentiation and involves the separation of an orthochromatic erythroblast into two daughter cells – a pyrenocyte containing the extruded nucleus, and a reticulocyte, which will become a red blood cell.
Newton LM, Lim KYB, Abeid DY, Wölwer CB, Johnson CJ, Russell SM, Hawkins ED, Humbert PO.   +7 more
europepmc   +3 more sources

Microtubule assembly by the Apc protein is regulated by importin-β—RanGTP [PDF]

Journal of Cell Science, 2010
Mutations in the tumour suppressor Adenomatous polyposis coli (Apc) initiate most sporadic colorectal cancers. Apc is implicated in regulating microtubule (MT) dynamics in interphase and mitosis. However, little is known about the underlying mechanism or regulation of this Apc function.
Dikovskaya, Dina, Li, Zhuoyu, Newton, Ian P., Davidson, Iain, Hutchins, James R. A., Kalab, Petr, Clarke, Paul R., Naethke, Inke S.   +7 more
openaire   +4 more sources

A non-canonical mechanism for Crm1-export cargo complex assembly

eLife, 2015
The transport receptor Crm1 mediates the export of diverse cargos containing leucine-rich nuclear export signals (NESs) through complex formation with RanGTP.
Ute Fischer, Nico Schäuble, Sabina Schütz, Martin Altvater, Yiming Chang, Marius Boulos Faza, Vikram Govind Panse   +6 more
doaj   +1 more source

Exportins can inhibit major mitotic assembly events in vitro: membrane fusion, nuclear pore formation, and spindle assembly

Nucleus, 2020
Xenopus egg extracts are a powerful in vitro tool for studying complex biological processes, including nuclear reconstitution, nuclear membrane and pore assembly, and spindle assembly.
Matthew S. Nord, Cyril Bernis, Sarah Carmona, Dennis C. Garland, Anna Travesa, Douglass J. Forbes   +5 more
doaj   +1 more source