Results 31 to 40 of about 3,329 (143)

Separable roles for RanGTP in nuclear and ciliary trafficking of a kinesin-2 subunit [PDF]

Journal of Biological Chemistry, 2021
Kinesin is part of the microtubule-binding motor protein superfamily, which serves important roles in cell division and intraorganellar transport. The heterotrimeric kinesin-2, consisting of the heterodimeric motor subunits, kinesin family member 3A/3B (KIF3A/3B), and kinesin-associated protein 3 (KAP3), is highly conserved across species from the ...
Shengping Huang, Larissa L. Dougherty, Prachee Avasthi   +2 more
openaire   +2 more sources

Microtubule assembly by the Apc protein is regulated by importin-β—RanGTP [PDF]

Journal of Cell Science, 2010
Mutations in the tumour suppressor Adenomatous polyposis coli (Apc) initiate most sporadic colorectal cancers. Apc is implicated in regulating microtubule (MT) dynamics in interphase and mitosis. However, little is known about the underlying mechanism or regulation of this Apc function.
Dikovskaya, Dina, Li, Zhuoyu, Newton, Ian P., Davidson, Iain, Hutchins, James R. A., Kalab, Petr, Clarke, Paul R., Naethke, Inke S.   +7 more
openaire   +4 more sources

NuSAP, a Mitotic RanGTP Target That Stabilizes and Cross-links Microtubules [PDF]

Molecular Biology of the Cell, 2006
Nucleolar and spindle-associated protein (NuSAP) was recently identified as a microtubule- and chromatin-binding protein in vertebrates that is nuclear during interphase. Small interfering RNA-mediated depletion of NuSAP resulted in aberrant spindle formation, missegregation of chromosomes, and ultimately blocked cell proliferation.
Ribbeck, K., Groen, A., Santarella, R., Bohnsack, M., Raemaekers, T., Köcher, T., Gentzel, M., Görlich, D., Wilm, M., Carmeliet, G., Mitchison, T., Ellenberg, J., Hoenger, A., Mattaj, I.   +13 more
openaire   +4 more sources

ISWI is a RanGTP-dependent MAP required for chromosome segregation [PDF]

Journal of Cell Biology, 2009
Production of RanGTP around chromosomes induces spindle assembly by activating nuclear localization signal (NLS)–containing factors. Here, we show that the NLS protein ISWI, a known chromatin-remodeling ATPase, is a RanGTP-dependent microtubule (MT)-associated protein. Recombinant ISWI induces MT nucleation, stabilization, and bundling in vitro.
Yokoyama, Hideki, Rybina, Sofia, Santarella-Mellwig, Rachel, Mattaj, Iain W., Karsenti, Eric   +4 more
openaire   +2 more sources

RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization [PDF]

Journal of Cell Biology, 2021
Zygotes require two accurate sets of parental chromosomes, one each from the mother and the father, to undergo normal embryogenesis. However, upon egg–sperm fusion in vertebrates, the zygote has three sets of chromosomes, one from the sperm and two from the egg.
Masashi Mori, Tatsuma Yao, Tappei Mishina, Hiromi Endoh, Masahito Tanaka, Nao Yonezawa, Yuta Shimamoto, Shigenobu Yonemura, Kazuo Yamagata, Tomoya S. Kitajima, Masahito Ikawa   +10 more
openaire   +2 more sources

RanGTP aids anaphase entry through Ubr5-mediated protein turnover [PDF]

Journal of Cell Biology, 2015
RanGTP is known to regulate the spindle assembly checkpoint (SAC), but the underlying molecular mechanism is unclear. BuGZ stabilizes SAC protein Bub3 through direct interaction and facilitates its mitotic function. Here we show that RanGTP promotes the turnover of BuGZ and Bub3 in metaphase, which in turn facilitates metaphase-to-anaphase transition ...
Jiang, Hao, He, Xiaonan, Feng, Di, Zhu, Xueliang, Zheng, Yixian   +4 more
openaire   +2 more sources

A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo [PDF]

The Journal of Cell Biology, 2003
The RanGTP gradient across the interphase nuclear envelope and on the condensed mitotic chromosomes is essential for many cellular processes, including nucleocytoplasmic transport and spindle assembly. Although the chromosome-associated enzyme RCC1 is responsible for RanGTP production, the mechanism of generating and maintaining the RanGTP gradient in ...
Li, Hoi Yeung, Wirtz, Denis, Zheng, Yixian   +2 more
openaire   +2 more sources

Role of SoxE transcription factors in development and disease

Developmental Dynamics, EarlyView.
Abstract Sox8, Sox9, and Sox10 arose by multiple rounds of genome duplications from a single SoxE gene in ancestral vertebrates. In this review, we will briefly discuss the molecular structure and function of SoxE transcription factors and their evolutionary origin. We will then discuss their expression, function, and developmental disorders.
Merin Lawrence, Gerhard Schlosser
wiley   +1 more source

The Specificity of the CRM1-Rev Nuclear Export Signal Interaction Is Mediated by RanGTP [PDF]

Journal of Biological Chemistry, 1998
Nuclear export of intron-containing human immunodeficiency virus type 1 (HIV-1) RNA is mediated by the viral Rev protein that contains both an RNA binding domain specific for the viral Rev response element (RRE) and a nuclear export signal (NES). The cellular CRM1 (Exportin1) protein functions as a nuclear export receptor for proteins carrying a Rev ...
P, Askjaer, T H, Jensen, J, Nilsson, L, Englmeier, J, Kjems   +4 more
openaire   +2 more sources

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, Volume 16, Issue 6, Page 1213-1221, June 2026.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source