Results 11 to 20 of about 6,495 (196)

The RanGTP pathway: from nucleo-cytoplasmic transport to spindle assembly and beyond [PDF]

Frontiers in Cell and Developmental Biology, 2016
The small GTPase Ran regulates the interaction of transport receptors with a number of cellular cargo proteins. The high affinity binding of the GTP-bound form of Ran to import receptors promotes cargo release, whereas its binding to export receptors ...
Tommaso eCavazza, Tommaso eCavazza, Isabelle eVernos, Isabelle eVernos, Isabelle eVernos   +4 more
doaj   +4 more sources

NuSAP Safeguards Centriole Integrity to Mediate CEP57‐CEP152 Torus Recruitment for Proper Engagement

Advanced Science
Precise centrosome regulation is crucial for faithful cell division, yet how microtubule‐associated proteins (MAPs) contribute to centrosome integrity remains poorly understood.
Shiyu Zhang, Zemin Jiang, Qiaoyun Yang, Hong Zheng, Minghao Wang, Chennianci Zhu, Lih‐Wen Deng, Karen Carmelina Crasta, Yih‐Cherng Liou   +8 more
doaj   +2 more sources

Induced opening of conformational switch I in GTP-bound Ran GTPase. [PDF]

Protein Sci
Abstract Ran is a small GTPase that regulates nucleocytoplasmic transport by cycling between an inactive GDP‐bound and active GTP‐bound state. Structurally, Ran consists of a globular G‐domain and a C‐terminal region that includes a C‐terminal helix. The switch I and II regions of the G‐domain undergo major conformational changes upon GTP hydrolysis ...
Czigleczki J, Dudas B, Balog E.
europepmc   +2 more sources

Histone Nuclear Import and Beyond: Multifunctional Roles of Importins. [PDF]

Bioessays
Efficient nuclear import of histones requires coordinated action between histone chaperones and nuclear import receptors. Importin‐4, Importin‐9, and Importin‐β/7 not only transport specific histone complexes but also act as chaperones, stabilizing histones and ensuring their protected delivery for nucleosome assembly, highlighting importins as central
Bernardes N, Chook YM.
europepmc   +2 more sources

γ-TuRC asymmetry induces local protofilament mismatch at the RanGTP-stimulated microtubule minus end

The EMBO Journal
The γ-tubulin ring complex (γ-TuRC) is a structural template for de novo microtubule assembly from α/β-tubulin units. The isolated vertebrate γ-TuRC assumes an asymmetric, open structure deviating from microtubule geometry, suggesting that γ-TuRC closure
Bram JA Vermeulen, Anna Böhler, Qi Gao, Annett Neuner, Erik Župa, Zhenzhen Chu, Martin Würtz, Ursula Jäkle, Oliver J Gruss, Stefan Pfeffer, Elmar Schiebel   +10 more
doaj   +3 more sources

Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex

Proceedings of the National Academy of Sciences, 2022
AbstractPreviously we showed that the nuclear import receptor Importin-9 wraps around the H2A-H2B core to chaperone and transport it from the cytoplasm to the nucleus. However, unlike most nuclear import systems where RanGTP dissociates cargoes from their importins, RanGTP binds stably to the Importin-9•H2A-H2B complex and formation of the ternary ...
Jenny Jiou, Joy M. Shaffer, Natalia E. Bernades, Ho Yee Joyce Fung, Juliana Kikumoto Dias, Sheena D’Arcy, Yuh Min Chook   +6 more
openaire   +2 more sources

A Novel Class of RanGTP Binding Proteins [PDF]

The Journal of Cell Biology, 1997
The importin-α/β complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. Although Ran has been implicated also in a variety of other processes, such as cell cycle progression, a direct function of Ran has so far only been demonstrated for importin-mediated nuclear import.
Görlich, D., Dabrowski, M., Bischoff, F., Kutay, U., Bork, P., Hartmann, E., Prehn, S., Izaurralde, E.   +7 more
openaire   +4 more sources

RanGTP aids anaphase entry through Ubr5-mediated protein turnover [PDF]

Journal of Cell Biology, 2015
RanGTP is known to regulate the spindle assembly checkpoint (SAC), but the underlying molecular mechanism is unclear. BuGZ stabilizes SAC protein Bub3 through direct interaction and facilitates its mitotic function. Here we show that RanGTP promotes the turnover of BuGZ and Bub3 in metaphase, which in turn facilitates metaphase-to-anaphase transition ...
Jiang, Hao, He, Xiaonan, Feng, Di, Zhu, Xueliang, Zheng, Yixian   +4 more
openaire   +2 more sources

RanGTP and CLASP1 cooperate to position the mitotic spindle [PDF]

Molecular Biology of the Cell, 2013
Accurate positioning of the mitotic spindle is critical to ensure proper distribution of chromosomes during cell division. The small GTPase Ran, which regulates a variety of processes throughout the cell cycle, including interphase nucleocytoplasmic transport and mitotic spindle assembly, was recently shown to also control spindle alignment.
Bird, Stephen L, Heald, Rebecca, Weis, Karsten   +2 more
openaire   +3 more sources

RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization [PDF]

Journal of Cell Biology, 2021
Zygotes require two accurate sets of parental chromosomes, one each from the mother and the father, to undergo normal embryogenesis. However, upon egg–sperm fusion in vertebrates, the zygote has three sets of chromosomes, one from the sperm and two from the egg.
Masashi Mori, Tatsuma Yao, Tappei Mishina, Hiromi Endoh, Masahito Tanaka, Nao Yonezawa, Yuta Shimamoto, Shigenobu Yonemura, Kazuo Yamagata, Tomoya S. Kitajima, Masahito Ikawa   +10 more
openaire   +2 more sources