Results 91 to 100 of about 40,965 (232)
ارزیابی تنوع ژنتیکی گندمهای دیپلوئید T. urartu بر اساس دادههای ریخت شناختی و نشانگرهای RAPD [PDF]
تنوع ژنتیکی در 22 مورفوتیپ گندم دیپلوئید T. urartu (ژنوم AA) با استفاده از صفات ریخت شناسی و نشانگرهای RAPD بررسی گردید. ارزیابیها براساس 17 صفت ریختشناسی و زراعی انجام شد و پارامترهای آماری صفات زراعی شامل پراکندگی و تنوع محاسبه گردید.
امیر عزیزیان +4 more
doaj
Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants
An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada. Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring. Greatest regeneration
A. Das, V. Kesari, L. Rangan
doaj +1 more source
Microwave treatment induced mutations and altered gene expression in Vigna aconitifolia
Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm-2) for 1, 3, 5 and 7 s before culturing.
R. K. Jangid +5 more
doaj +1 more source
OPTIMIZATION OF THE SYSTEM AND PROCEDURE OF R.DOMINICA RAPD REACTION
以谷蠹为研究对象,采用L16(45)正交组合实验和单因素梯度实验对MgCl2、dNTP、随机引物、Taq酶、模板DNA浓度和退火温度、循环次数等影响RAPD扩增的重要因素进行优化,以期建立最优的RAPD反应体系与程序。实验结果表明:各因素最适条件为:25μL PCR反应体系中,10×Buffer 2.5μL,MgCl22.5 mmol/L,dNTP0.4 mmol/L,随机引物960 pmol/L,Taq酶0.5 U,模板DNA 20ng;退火温度为37℃,循环次数为45次。在对谷蠹进行RAPD分析时 ...
余懋群, 吴芳, 龙海
core
Physiological and Molecular Response of Padina pavonica (Phaeophyta) brown Alga Towards Cadmium Heavy Metal [PDF]
Physiological and molecular response of Padina pavonica (phaeophyta) marine alga exposed to different cadmium (0, 2.5, 5 and 10 mg/L) concentrations after 4 days of exposure has been investigated.
Basel Saleh
doaj
Partial map of Coffea arabica L. and recovery of the recurrent parent in backcross progenies
A partial map of Coffea arabica L. was constructed based on a backcross population and RAPD markers. From a total of 178 markers evaluated, only 134 that segregated 1:1 (P>0.05) were used to develop the map.
Antonio Carlos Baião de Oliveira +5 more
doaj
Metapenaeus affinis a species from Metapenaeus genera of Penaeidae shrimps in khaur Abdullah (salty water southern of Iraq), morphologically closely related organism with suspected member in Shatt al-Arab(freshwater)also southern of Iraq.
Alzuhairi Rabeeha, Rabeeha Alzuhairi
core +1 more source
Molecular analysis of the seedlessness character in grape using RAPD markers
Forty-eight polymorphic RAPD primers were used to study the genetic similarity of eight grapevine (Vitis spp.) accessions: Itália, Seyve Villard 12327, Seyve Villard 12375, Crimson Seedless, CG 87746, A 1976, Gota de Ouro and CNPUV 154-27.
Júlio César de Lima +4 more
doaj
随机选用了 10条RAPD引物 ,采用 3种商品TaqDNA聚合酶体系 ,以 5个不同物种的动植物总DNA为模板 ,进行RAPD扩增 .实验发现 ,在对同一模板、采用同一引物进行的RAPD扩增中 ,仅仅因为使用了不同的商品TaqDNA聚合酶 ,获得的扩增产物差别极大 ;不同商品来源的PCR反应缓冲体系 ,对扩增产物也有一定影响 ,但相对较小 .这说明 ,不同的商品酶体系对RAPD扩增产物影响很大 .因此 ,影响RAPD扩增产物稳定性的一个关键因素是商品TaqDNA聚合酶本身 ...
何礼,袁水全,莫邦辉,姚光贵,林宏辉,陈放,郭骁才 +1 more
core
The objective of this study was to identify molecular markers associated with the resistance to race 3 of the SCN. Two microsatellites (Satt187 and Satt309) and three RAPD markers (OPAG-05946, OPF-041038, and OPAQ-011987) were found which explained 31.3%,
Valdemar de Paula Carvalho +4 more
doaj

