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Innovative and rapid antimicrobial susceptibility testing systems [PDF]

Nature Reviews Microbiology, 2020
Antimicrobial resistance (AMR) is a major threat to human health worldwide, and the rapid detection and quantification of resistance, combined with antimicrobial stewardship, are key interventions to combat the spread and emergence of AMR. Antimicrobial susceptibility testing (AST) systems are the collective set of diagnostic processes that facilitate ...
Alex Van Belkum, Carey-Ann D Burnham, John W A Rossen   +2 more
exaly   +5 more sources

Rapid antimicrobial susceptibility tests for sepsis; the road ahead

Journal of Medical Microbiology, 2019
Current methods for antimicrobial susceptibility testing (AST) are too slow to affect initial treatment decisions in the early stages of sepsis, when the prescriber is most concerned to select effective therapy immediately, rather than finding out what will not work 1 or 2 days later.
Oskar Ekelund, Timothy Inglis
exaly   +3 more sources

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Recent advances in rapid antimicrobial susceptibility testing systems

Expert Review of Molecular Diagnostics, 2021
Until recently antimicrobial susceptibility testing (AST) methods based on the demonstration of phenotypic susceptibility in 16-24 h remained largely unchanged.Advances in rapid phenotypic and molecular-based AST systems.AST has changed over the past decade, with many rapid phenotypic and molecular methods developed to demonstrate phenotypic or ...
Michael R. Jacobs, Jordan D. Colson, Daniel D. Rhoads   +2 more
openaire   +2 more sources

Combinatorial screening SlipChip for rapid phenotypic antimicrobial susceptibility testing

Lab on a Chip, 2022
A combinatorial-screening SlipChip based on a self-partitioning mechanism performs high-throughput phenotypic AST with a panel of antibiotics and antibiotic combinations in three hours by monitoring the phenotypic profile of bacterial growth.
Xiang Li, Xu Liu, Ziqing Yu, Yang Luo, Qixin Hu, Zhenye Xu, Jia Dai, Nannan Wu, Feng Shen   +8 more
openaire   +2 more sources

Rapid Antimicrobial Susceptibility Testing Based on a Bio-Inspired Chemiluminescence Sensor

Analytical Chemistry, 2022
Indiscriminate usage of antibiotics has caused accelerating growth and global expansion of antimicrobial resistance. Therefore, rapid antimicrobial susceptibility testing (AST) for guiding antibiotic prescription and preventing the spread of antimicrobial resistance is in urgent need.
Jidong Wang, Ping Hui, Xinyu Zhang, Xiaoqing Cai, Jie Lian, Xiaolei Liu, Xi Lu, Wenwen Chen   +7 more
openaire   +2 more sources

Evaluation of a bioluminescence assay for rapid antimicrobial susceptibility testing of mycobacteria

European Journal of Clinical Microbiology, 1985
The antimicrobial susceptibility of Mycobacterium tuberculosis H37Rv and 17 clinical isolates of the same species was determined using a bioluminescence assay to measure adenosine triphosphate (ATP) produced by bacteria in the broth dilution test performed in Dubos-Tween-albumin broth.
B, Beckers, H R, Lang, D, Schimke, A, Lammers   +3 more
openaire   +2 more sources

An integrated microfluidic chip for rapid and multiple antimicrobial susceptibility testing

The Analyst
We introduce an integrated microfluidic platform, enabling multiple antimicrobial susceptibility testing (AST) and minimum inhibitory concentration (MIC) determination after 2 hours of incubation.
Zirui Pang, Lulu Shi, Mingyu Wang, Jifang Tao   +3 more
openaire   +2 more sources

Rapid susceptibility testing on urine for antimicrobial stewardship in general practice

Journal of Antimicrobial Chemotherapy
Abstract Objective Rapid antimicrobial susceptibility testing (AST) can improve antimicrobial management and patient outcome. Urinary tract infection (UTI) is one of the most common reasons for antimicrobial prescription in general practice.
Patrick D J, Sturm, Tanja, Schülin
openaire   +2 more sources

Blood culture-free ultra-rapid antimicrobial susceptibility testing

Nature
Treatment assessment and patient outcome for sepsis depend predominantly on the timely administration of appropriate antibiotics1-3. However, the clinical protocols used to stratify and select patient-specific optimal therapy are extremely slow4. In particular, the major hurdle in performing rapid antimicrobial susceptibility testing (AST) remains in ...
Tae Hyun Kim, Junwon Kang, Haewook Jang, Hyelyn Joo, Gi Yoon Lee, Hamin Kim, Untack Cho, Hyeeun Bang, Jisung Jang, Sangkwon Han, Dong Young Kim, Chan Mi Lee, Chang Kyung Kang, Pyoeng Gyun Choe, Nam Joong Kim, Myoung-don Oh, Taek Soo Kim, Inho Kim, Wan Beom Park, Sunghoon Kwon   +19 more
openaire   +2 more sources