Results 71 to 80 of about 1,136,282 (266)

Cloning of the Coat Protein Gene of Little Cherry Virus 1 and the Preparation of a Polyclonal Antibody

International Journal of Fruit Science
Little cherry disease (LChD) is a serious viral disease that significantly reduces the yield and quality of sweet cherries. And Little cherry virus 1 (LChV-1) is a major pathogen. To facilitate the rapid and efficient detection of LChV-1, we successfully
Wei Li, Qinwang Shang, Xiaohou Xu, Qingle Chang, Deya Wang   +4 more
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Rapid identification of Lonicera japonica via Proofman-LMTIA technology

Scientific Reports
The study introduces a novel, rapid and precise method for identifying Lonicera japonica (JYH), an important medicinal herb, utilizing Proofman-LMTIA (Proofman probe-ladder melting temperature isothermal amplification) technology. Based on the 5.8 S-ITS2
Xiaodong Zhang, Caixia Li, Dejia Lan, Jinxin Liu, Deguo Wang   +4 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

Frontiers in Microbiology, 2019
The accurate and rapid detection of Campylobacter spp. is critical for optimal surveillance throughout poultry processing in the United States. The further development of highly specific and sensitive assays to detect Campylobacter in poultry matrices ...
S. Ricke, K. Feye, W. Chaney, Zhaohao Shi, H. Pavlidis, Yichao Yang   +5 more
semanticscholar   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Ultrasound-Assisted and Citric Acid-Guided Creation of ZnO Nanoparticles with Optimized Morphologies to Boost Malachite Green Photocatalysis

Molecules
Zinc oxide (ZnO) semiconductors are renowned for their cost-effective synthesis and superior catalytic attributes, making them prominent in environmental remediation applications. This study presents the synthesis of ZnO nanoparticles (NPs) with distinct
Xianlu Lei, Shuang Li, Jian Zeng, Meiqi Huang, Miaomiao Ma, Xueyan Ran, Xiang Chen, Yuting Yin, Qi Sun, Tao Le   +9 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Ultrasensitive and rapid detection of ochratoxin A in agro-products by a nanobody-mediated FRET-based immunosensor.

Journal of Hazardous Materials, 2019
Ochratoxin A (OTA) is a major concern for public health and the rapid detection of trace OTA in food is always a challenge. To minimize OTA exposure to consumers, a nanobody (Nb)-mediated förster resonance energy transfer (FRET)-based immunosensor using ...
Zongwen Tang, Xing Liu, Benchao Su, Qi Chen, Hongmei Cao, Yonghuan Yun, Yang Xu, B. Hammock   +7 more
semanticscholar   +1 more source