Results 91 to 100 of about 1,231,297 (284)

Translates of rational points along expanding closed horocycles on the modular surface. [PDF]

Math Ann, 2022
Burrin C, Shapira U, Yu S.
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Rational points and rational moduli spaces

v2 added more motivation/explanations for general constructions. This paper is based on the two papers (arXiv:1904.03503, arXiv:2307.06944) with Arno Kret: All three papers are now submitted, comments are always very ...
Fan, Shijie, von Kanel, Rafael
openaire   +2 more sources

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Rational cohomology fixed points

Advances in Group Theory and Applications, 2023
This work concerns rational homotopy. Let \((\land V, d)\) be the Sullivan minimal model of a simply connected CW-complex, of finite type, \(X\). Denote \({\mathcal{E}_{\sharp}}(\land V,d)\) the group of homotopy self equivalences inducing the identity on \(V\). By definition, the space \(X\) has an \(m\)-rational cohomology fixed point if there exist \
openaire   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

A rational optimal block hybrid method for enhanced accuracy in solving Lane–Emden equations

Partial Differential Equations in Applied Mathematics
This paper introduces a block hybrid method designed for the effective resolution of Lane–Emden equations, which are characterized as second-order boundary value problems incorporating a singularity at the origin.
Sandile Motsa, Salma Ahmedai, Mpho Nefale, Olumuyiwa Otegbeye   +3 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Optimal spectroscopic measurement design: Bayesian framework for rational data acquisition

Machine Learning: Science and Technology
We propose an optimal experimental design method for spectroscopic measurements that can determine the appropriate number and placement of measurement points in a rational manner.
Yusei Ito, Yasuo Takeichi, Hideitsu Hino, Kanta Ono   +3 more
doaj   +1 more source