Results 141 to 150 of about 798,166 (289)

Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells

Journal of Immunology Research, 2017
Objective. This study aimed to explore the immunoregulatory effect of flavonoids of blueberry (Vaccinium corymbosum L.) leaves (FBL). Methods. The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance ...
Dazhi Shi, Mengyi Xu, Mengyue Ren, Enshan Pan, Chaohua Luo, Wei Zhang, Qingfa Tang   +6 more
doaj   +1 more source

Supplemental Figure 4: CRISPR/Cas9 HuR ko in RAW 264.7 macrophage cells. from HuR Small-Molecule Inhibitor Elicits Differential Effects in Adenomatosis Polyposis and Colorectal Carcinogenesis

, 2017
CRISPR/Cas9 HuR ko in RAW 264.7 macrophage cells confirmed by Sanger sequencing and Western blot analysis.
Orest Kuzyk (14923293), Vineeta Khare (117537), Guglielmo Roma (155263), Alexander Loy (139849), Rayko Evstatiev (326925), Christoph Gasche (117548), David Berry (346175), Sabin Bhuju (107606), Ildiko Mesteri (577551), Michaela Lang (117542), Nicole Meisner-Kober (14923296), Katharina Passecker (14923290), Kyle Dammann (117541), Pavel Kovarik (220565), Vitaly Sedlyarov (9090320), Martin Beibel (3480251), Florian Ebner (4576525)   +16 more
core   +1 more source

Spatiotemporally Precise Rheumatoid Arthritis Therapy via a Triple‐Locked Cascaded Nanoplatform With Dual‐Channel Self‐Reporting

Advanced Science, EarlyView.
An orthogonally programmable nanotheranostic (MB@YW NPs) enables precision therapy for rheumatoid arthritis. A triple‐locked (pH, HOCl/ONOO−, light) independently drives the release of methotrexate and sulfasalazine. Trackable by dual‐channel fluorescence, this coordinated drug delivery remodels the inflammatory microenvironment to markedly suppress ...
Jihong Liu, Wendi Zhu, Shiyun Xu, Shichang Liu, Wenjun Tang, Xiaoyu Ma, Ting Yang, Haojian Yang, Hongtong Wang, Lu Cao, Lijie Tan, Qi Wang   +11 more
wiley   +1 more source

Intrabody expression in stably transfected RAW 264.7 macrophages and dendritic D1 cells.

, 2019
A, Expression of αIFNα-ib in RAW 264.7 macrophages analysed by immunoblot after immunoprecipitation. For immunoprecipitation, 106 RAW-αIFNα-ib-eGFP cells were cultured in a 6-well microtitre plate for 48 h.
Joop Van den Heuvel (6593702), Paul Pentrowski (6593690), Mario Köster (441538), Konrad Büssow (4871), Andrea Kröger (373919), Philipp Themann (6593684), Rainer Zawatzky (277657), Thomas Böldicke (4023743), Claudia Harting (6593693), Veith Vollmer (6593699), Sabine Luu (6593687), Mira Majewski (6593696), Martina Grashoff (544284)   +12 more
core   +1 more source

Lithium‐Doped Hydroxyapatite Nanowires Rejuvenate Senescent Macrophages Via the Lysosome‐Dependent Ferroptosis Axis to Promote Aged Bone Regeneration

Advanced Science, EarlyView.
Li‐nHAP+GelMA promotes mandibular bone regeneration in aged mice by remodeling the senescent immune microenvironment. Local delivery of Li‐nHAP restores lysosomal homeostasis in senescent macrophages, reduces intracellular labile Fe2+ accumulation, ROS production, and lipid peroxidation, thereby suppressing macrophage ferroptosis and senescence ...
Baixi Chen, Ziduan Ding, Ruomei Li, Cheng Zhu, Zhiyu Zhu, Zitong Yang, Luyao Qu, Jin Hao, Lunguo Xia, Bing Fang, Lu Liu   +10 more
wiley   +1 more source

Internalization of CPP by RAW 264.7 cells.

, 2013
A, RAW 264.7 macrophage were seeded on coverslips and incubated with 100 µg/mL AlexaFluor488-labeled fetuin-A-containing synthetic CPP (green) in DMEM culture medium supplemented with 10% FBS for 30 minutes at 37°C, with (right-hand side panel) or ...
Eric Hanssen (349226), Stephen G. Holt (400521), Lawrence P. McMahon (400520), Edward R. Smith (400519)   +3 more
core   +1 more source

Biomimetic Nanoplatform for Fibrosis Remodeling and Anti‐PD‐1 Potentiation via Cu+/Ca2+ Ion Dysregulation and TGF‐β Inhibition in PDAC

Advanced Science, EarlyView.
A biomimetic nanoplatform (RPm@CC‐LY) remodels fibrotic stroma via sustained TGF‐β inhibition, triggers oxidative stress and apoptosis through Cu+‐mediated Fenton chemistry and Ca2+ overload, and reprograms macrophages and dendritic cells to boost CD8+ T cell infiltration.
Jianmei Li, Qinlong Wen, Jie Dai, Li Yang, Feng Xiong, Zhigong Wei, Xingchen Peng   +6 more
wiley   +1 more source

Phase‐Programmed Microneedles Enable Sequential Hydrogen and Quercetin Release for Scar‐Reduced Regeneration of Burn Wounds

Advanced Science, EarlyView.
A phase‐programmed microneedle patch, comprising a poly (lactic‐co‐glycolic acid) (PLGA) shell and a sulfobutylether‐β‐cyclodextrin (SBECD) core, is designed for sequential hydrogen and quercetin release for scar‐reduced regeneration of burn wounds. ABSTRACT Burn wounds complicated by bacterial infection and fibrotic scarring remain a major clinical ...
Yongnian Zeng, Zhigang Chu, Huiyuan Yang, Wen Zhang, Peng Jiang, Jiapeng Lei, Siyu Zhang, Qiongfang Ruan, Shuhua Liu, Wei Li   +9 more
wiley   +1 more source

pH‐Responsive Ultrasmall Iron Oxide Nanoassemblies for Activatable T2/T1 Magnetic Resonance Imaging

Advanced NanoBiomed Research, EarlyView.
This research reports a pH‐responsive ultrasmall iron oxide nanoparticle (USIONs)/polymer nanoassemblies for activatable magnetic resonance imaging. Amphiphilic diphosphonic acid block copolymers compactly encapsulate USIONs to generate T2‐weighted contrast at physiological pH, while acidic conditions trigger disassembly, activating T1‐weighted ...
Xumin Huang, Liwei Liu, Jiayuan Zhu, Xinghao Hu, Changkui Fu, Yao Wang, Lining Arnold Ju, Thomas P. Davis, Andriansjah Rukmana, Shaohua Ma, Zheng Zhang, Ruirui Qiao   +11 more
wiley   +1 more source

HMGB1 induces an inflammatory response in RAW 264.7 macrophages.

, 2013
A–B: RAW 264.7 cells were treated with the indicated amount of HMGB1 and IFN-γ for 18 hours and the TNFα secretion was measured in the supernatant.
Sangeeta Chevan (422090), Domokos Gerö (422086), Huan Yang (36076), Katalin Módis (422088), Kevin J. Tracey (250284), Yousef Al-Abed (93844), Csaba Szabo (422091), John P. Pribis (422089), Petra Szoleczky (422087), Timothy R. Billiar (91972)   +9 more
core   +1 more source