Results 101 to 110 of about 5,986,284 (284)

Toxicity of PAO1 toward RAW 264.7 cells.

open access: yes, 2013
A) RAW 264.7 cells were exposed to PAO1 in the presence of different kinase inhibitors: SP600125 (for JNK, 10 mg/ml), U0126 (for MEK, 10 mg/ml) and BMS345541 (for IKK, 10 mg/ml). MTT assays quantitated cell viability after 1 day.
Alan M. Tartakoff (276669)   +2 more
core   +1 more source

A Dual‐Membrane Biomimetic Nanoplatform Enables Triple‐Modal Therapy Against SARS‐CoV‐2 Through Viral Decoy, Inflammation Neutralizing, and Intracellular RNAi

open access: yesAdvanced Science, EarlyView.
[A&T]MLN is a triple‐modal nanoplatform with siRNA‐loaded LN coated with hybrid ACE2/macrophage membrane. It blocks viral entry, neutralizes IL‐6/IL‐1β/TNF‐α, and delivers siRNA to suppress viral replication. In a murine lung injury model, it attenuates inflammation, offering a multi‐pronged strategy against SARS‐CoV‐2 variants and hyperinflammation ...
Hui Li   +19 more
wiley   +1 more source

A CD14‐Targeting In Situ Hydrogel Directs Macrophage Reprogramming to Minimize Scar Formation

open access: yesAdvanced Science, EarlyView.
A CD14‐targeting in situ hydrogel formed from a microbial polysaccharide rapidly remodels the immune microenvironment by reprogramming macrophage polarization, suppressing excessive inflammation, and coordinating tissue regeneration. This multifunctional hydrogel promotes scar‐reduced wound repair through enhanced angiogenesis, collagen remodeling, and
Rui Fang   +5 more
wiley   +1 more source

Lipopolysaccharide Induces Hyporesponsiveness to Its Own Action in RAW 264.7 Cells

open access: yesJournal of Biological Chemistry, 1989
Bacterial endotoxin (lipopolysaccharide, LPS) has the property of inducing hyporesponsiveness or tolerance to its own effects. This phenomenon has been demonstrated in man and experimental animals. The cellular changes that contribute to LPS tolerance are not understood.
G D, Virca   +3 more
openaire   +2 more sources

Restoring Endothelial Mitochondrial Homeostasis With a Bioactive Nanoshield Promotes Vascular Regeneration on Cardiovascular Implants

open access: yesAdvanced Science, EarlyView.
A bioactive poly(citrate‐polyglycol‐procyanidin) (PCGP) nanoshield is developed for cardiovascular implants to restore endothelial mitochondrial homeostasis following vascular injury. By preserving mitochondrial integrity, re‐establishing metabolic balance, and activating PI3K/Akt/eNOS‐NO signaling, the PCGP interface promotes functional endothelial ...
Xiyu Liu   +14 more
wiley   +1 more source

RAW 264.7 Cell Culture Protocol

open access: yes
This document provides a brief Standard Operating Procedure (SOP) for the culture of RAW 264.7 cells. It summarizes their origin and growth requirements to support students in planning experiments and maintaining good cell culture practice. The aim is to promote reproducibility, avoid common pitfalls in mammalian cell culture, and ensure responsible ...
openaire   +1 more source

Multifunctional Zinc Glycyrrhizinate‐Integrated Gel for Enhanced Probiotic Delivery and Alleviation of Acute and Spontaneous Colitis

open access: yesAdvanced Science, EarlyView.
Multifunctional oral delivery system—L. reuteri@ZGM—act as both a carrier and therapeutic adjuvant, providing ROS scavenging, and mucin‐interactive properties tailored to the IBD microenvironment. This bioactive gel safeguards L. reuteri from gastric degradation, enhances mucosal adhesion, and promotes long‐term retention at inflamed sites, thereby ...
Mengyun Peng   +8 more
wiley   +1 more source

Using Image J to Evaluate Cell Number in Cultured RAW 264.7 Macrophages

open access: yes, 2020
A comparison of the response of RAW 264.7 cells to bone marrow derived macrophages (BMDM) stimulated with bacterial lipopolysaccharide O55:B5 from E. coli (LPS) intended to determine if the cell line and primary cells demonstrated equivalent performance ...
Gallagher, Tyler
core   +1 more source

4-hexylresorcinol-induced protein expression changes in human umbilical cord vein endothelial cells as determined by immunoprecipitation high-performance liquid chromatography.

open access: yesPLoS ONE, 2020
4-Hexylresorcinol (4HR) is used as a food preservative and an ingredient of toothpaste and cosmetics. The present study was performed using 233 antisera to determine the changes in protein expression induced by 4HR in human umbilical cord vein ...
Yeon Sook Kim   +3 more
doaj   +1 more source

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