Results 51 to 60 of about 12,504 (200)

RATEX: A Scalable RNA‐Based Platform for Logical and Multi‐Layered Cellular Programming

Angewandte Chemie, EarlyView.
RATEX integrated ribosome‐mediated transcription control with synthetic RNA regulators, enabling simultaneous processing of RNA, metabolite, and small‐molecule inputs through complex logic operations within a single transcript. The platform achieves up to 1,492‐fold gene regulation, multi‐input hybrid logic gates, RNA‐programmed signaling cascades, and
Hyunseop Goh, Hansol Kang, Chaeri Kim, Jongmin Kim   +3 more
wiley   +2 more sources

Versatile dual reporter gene systems for investigating stop codon readthrough in plants. [PDF]

PLoS ONE, 2009
Translation is most often terminated when a ribosome encounters the first in-frame stop codon (UAA, UAG or UGA) in an mRNA. However, many viruses (and some cellular mRNAs) contain "stop" codons that cause a proportion of ribosomes to terminate and others
Nga T Lao, Alan P Maloney, John F Atkins, Tony A Kavanagh   +3 more
doaj   +1 more source

Genetic basis of hidden phenotypic variation revealed by increased translational readthrough in yeast. [PDF]

PLoS Genetics, 2012
Eukaryotic release factors 1 and 3, encoded by SUP45 and SUP35, respectively, in Saccharomyces cerevisiae, are required for translation termination. Recent studies have shown that, besides these two key factors, several genetic and epigenetic mechanisms ...
Noorossadat Torabi, Leonid Kruglyak
doaj   +1 more source

Stress-induced transcriptional readthrough into neighboring genes is linked to intron retention

bioRxiv, 2022
Exposure to certain stresses leads to readthrough transcription. Here we found that readthrough transcription often proceeds into the proximal gene downstream, in a phenomenon termed “read-in”. Using polyA-selected RNA-seq data from mouse fibroblasts, we
Shani Hadar, A. Meller, Reut Shalgi
semanticscholar   +1 more source

The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code [PDF]

Open Biology, 2016
Translational readthrough gives rise to C-terminally extended proteins, thereby providing the cell with new protein isoforms. These may have different properties from the parental proteins if the extensions contain functional domains.
Julia Hofhuis, Fabian Schueren, Christopher Nötzel, Thomas Lingner, Jutta Gärtner, Olaf Jahn, Sven Thoms   +6 more
doaj   +1 more source

Recognition of 3′ nucleotide context and stop codon readthrough are determined during mRNA translation elongation

Journal of Biological Chemistry, 2022
The nucleotide context surrounding stop codons significantly affects the efficiency of translation termination. In eukaryotes, various 3′ contexts that are unfavorable for translation termination have been described; however, the exact molecular ...
N. Biziaev, E. Sokolova, D. Yanvarev, I. Toropygin, A. Shuvalov, T. Egorova, E. Alkalaeva   +6 more
semanticscholar   +1 more source

Nonaminoglycoside compounds induce readthrough of nonsense mutations [PDF]

Journal of Experimental Medicine, 2009
Large numbers of genetic disorders are caused by nonsense mutations for which compound-induced readthrough of premature termination codons (PTCs) might be exploited as a potential treatment strategy. We have successfully developed a sensitive and quantitative high-throughput screening (HTS) assay, protein transcription/translation (PTT)–enzyme-linked ...
Du, Liutao, Damoiseaux, Robert, Nahas, Shareef, Gao, Kun, Hu, Hailiang, Pollard, Julianne M, Goldstine, Jimena, Jung, Michael E, Henning, Susanne M, Bertoni, Carmen, Gatti, Richard A   +10 more
openaire   +4 more sources

Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency

Molecular Neurodegeneration, 2020
Background Frontotemporal lobar degeneration (FTLD) is a devastating and progressive disorder, and a common cause of early onset dementia. Progranulin (PGRN) haploinsufficiency due to autosomal dominant mutations in the progranulin gene (GRN) is an ...
Jonathan Frew, Alireza Baradaran-Heravi, Aruna D. Balgi, Xiujuan Wu, Tyler D. Yan, Steve Arns, Fahimeh S. Shidmoossavee, Jason Tan, James B. Jaquith, Karen R. Jansen-West, Francis C. Lynn, Fen-Biao Gao, Leonard Petrucelli, Howard H. Feldman, Ian R. Mackenzie, Michel Roberge, Haakon B. Nygaard   +16 more
doaj   +1 more source

Recoding of Nonsense Mutation as a Pharmacological Strategy

Biomedicines, 2023
Approximately 11% of genetic human diseases are caused by nonsense mutations that introduce a premature termination codon (PTC) into the coding sequence. The PTC results in the production of a potentially harmful shortened polypeptide and activation of a
Gazmend Temaj, Pelin Telkoparan-Akillilar, Nexhibe Nuhii, Silvia Chichiarelli, Sarmistha Saha, Luciano Saso   +5 more
doaj   +1 more source

Translational readthrough of nonsense mutant TP53 by mRNA incorporation of 5-Fluorouridine

Cell Death and Disease, 2022
TP53 nonsense mutations in cancer produce truncated inactive p53 protein. We show that 5-FU metabolite 5-Fluorouridine (FUr) induces full-length p53 in human tumor cells carrying R213X nonsense mutant TP53.
Mireia Palomar-Siles, Angelos Heldin, Meiqiongzi Zhang, Charlotte Strandgren, Viktor Yurevych, Jip T. van Dinter, S. Engels, Damon A. Hofman, S. Öhlin, B. Meineke, V. Bykov, Sebastiaan van Heesch, K. Wiman   +12 more
semanticscholar   +1 more source