Results 41 to 50 of about 12,543 (199)

Stop codon context influences genome-wide stimulation of termination codon readthrough by aminoglycosides

eLife, 2020
Stop codon readthrough (SCR) occurs when the ribosome miscodes at a stop codon. Such readthrough events can be therapeutically desirable when a premature termination codon (PTC) is found in a critical gene.
Jamie R Wangen, Rachel Green
doaj   +1 more source

Site‐Specific Protein Bioconjugation Through Cellular Incorporation of Noncanonical Amino Acids

Angewandte Chemie, EarlyView.
Genetic code expansion (GCE) enables site‐specific installation of noncanonical amino acids containing bioorthogonal conjugation handles, allowing precise, homogeneous protein modification. This review examines the principles of orthogonal translation, surveys the chemistries available for chemoselective labeling, and highlights emerging multi‐site ...
Rahul Sarkar, Manas Joshi, Abhishek Chatterjee   +2 more
wiley   +2 more sources

Novel compounds that synergize with aminoglycoside G418 or eRF3 degraders for translational readthrough of nonsense mutant TP53 and PTEN

RNA Biology, 2023
The TP53 and PTEN tumour suppressor genes are inactivated by nonsense mutations in a significant fraction of human tumours. TP53 nonsense mutant tumours account for approximately one million new cancer cases per year worldwide.
Angelos Heldin, Matko Cancer, Mireia Palomar-Siles, Susanne Öhlin, Meiqiongzi Zhang, Alexander Sun-Zhang, Anna Mariani, Jianping Liu, Vladimir J.N. Bykov, Klas G. Wiman   +9 more
doaj   +1 more source

Functional Restoration of BRCA1 Nonsense Mutations by Aminoglycoside-Induced Readthrough

Frontiers in Pharmacology, 2022
BRCA1 is a major tumor suppressor that functions in the accurate repair of DNA double-strand breaks via homologous recombination (HR). Nonsense mutations in BRCA1 lead to inactive truncated protein products and are associated with high risk of breast and
Renata B. V. Abreu, Thiago T. Gomes, Thales C. Nepomuceno, Thales C. Nepomuceno, Xueli Li, Mateus Fuchshuber-Moraes, Giuliana De Gregoriis, Guilherme Suarez-Kurtz, Alvaro N. A. Monteiro, Marcelo A. Carvalho, Marcelo A. Carvalho   +10 more
doaj   +1 more source

Genetic basis of hidden phenotypic variation revealed by increased translational readthrough in yeast. [PDF]

PLoS Genetics, 2012
Eukaryotic release factors 1 and 3, encoded by SUP45 and SUP35, respectively, in Saccharomyces cerevisiae, are required for translation termination. Recent studies have shown that, besides these two key factors, several genetic and epigenetic mechanisms ...
Noorossadat Torabi, Leonid Kruglyak
doaj   +1 more source

Versatile dual reporter gene systems for investigating stop codon readthrough in plants. [PDF]

PLoS ONE, 2009
Translation is most often terminated when a ribosome encounters the first in-frame stop codon (UAA, UAG or UGA) in an mRNA. However, many viruses (and some cellular mRNAs) contain "stop" codons that cause a proportion of ribosomes to terminate and others
Nga T Lao, Alan P Maloney, John F Atkins, Tony A Kavanagh   +3 more
doaj   +1 more source

Recoding of Nonsense Mutation as a Pharmacological Strategy

Biomedicines, 2023
Approximately 11% of genetic human diseases are caused by nonsense mutations that introduce a premature termination codon (PTC) into the coding sequence. The PTC results in the production of a potentially harmful shortened polypeptide and activation of a
Gazmend Temaj, Pelin Telkoparan-Akillilar, Nexhibe Nuhii, Silvia Chichiarelli, Sarmistha Saha, Luciano Saso   +5 more
doaj   +1 more source

Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency

Molecular Neurodegeneration, 2020
Background Frontotemporal lobar degeneration (FTLD) is a devastating and progressive disorder, and a common cause of early onset dementia. Progranulin (PGRN) haploinsufficiency due to autosomal dominant mutations in the progranulin gene (GRN) is an ...
Jonathan Frew, Alireza Baradaran-Heravi, Aruna D. Balgi, Xiujuan Wu, Tyler D. Yan, Steve Arns, Fahimeh S. Shidmoossavee, Jason Tan, James B. Jaquith, Karen R. Jansen-West, Francis C. Lynn, Fen-Biao Gao, Leonard Petrucelli, Howard H. Feldman, Ian R. Mackenzie, Michel Roberge, Haakon B. Nygaard   +16 more
doaj   +1 more source

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals

eLife, 2014
Translational readthrough gives rise to low abundance proteins with C-terminal extensions beyond the stop codon. To identify functional translational readthrough, we estimated the readthrough propensity (RTP) of all stop codon contexts of the human ...
Fabian Schueren, Thomas Lingner, Rosemol George, Julia Hofhuis, Corinna Dickel, Jutta Gärtner, Sven Thoms   +6 more
doaj   +1 more source

Nonsense mutation suppression is enhanced by targeting different stages of the protein synthesis process.

PLoS Biology, 2023
The introduction of premature termination codons (PTCs), as a result of splicing defects, insertions, deletions, or point mutations (also termed nonsense mutations), lead to numerous genetic diseases, ranging from rare neuro-metabolic disorders to ...
Amnon Wittenstein, Michal Caspi, Ido Rippin, Orna Elroy-Stein, Hagit Eldar-Finkelman, Sven Thoms, Rina Rosin-Arbesfeld   +6 more
doaj   +1 more source