Results 81 to 90 of about 12,504 (200)

Comparative evaluation of noncanonical amino acids as site‐specific NMR probes for the complex of E. coli SSB with single‐stranded DNA without isotope labelling

The FEBS Journal, EarlyView.
Genetic encoding of noncanonical amino acids (ncAA) enables their site‐specific installation in proteins. This work compares the nuclear magnetic resonance (NMR) performance of different ncAAs in a large protein−DNA complex (~ 100 kDa) without isotope labelling. The ncAAs deliver readily detectable NMR signals in the complex between E.
Sreelakshmi Mekkattu Tharayil, Haocheng Qianzhu, Elwy H. Abdelkader, Adarshi P. Welegedara, Josemon George, Christoph Nitsche, Thomas Huber, Gottfried Otting   +7 more
wiley   +1 more source

Ribosomal frameshifting, jumping and readthrough

Current Opinion in Cell Biology, 1991
New examples of high-level ribosomal frameshift and readthrough events have been described over the past year and a half. These include -1 frameshifting at tandem codons and +1 frameshifting at neighboring slow codons. Several bizarre examples of ribosome jumping and multiple stop-codon readthrough continue to perplex investigators in this field.
openaire   +2 more sources

Translational readthrough in Tobacco necrosis virus-D

Virology, 2014
The plus-strand RNA genome of Tobacco necrosis virus-D (TNV-D) expresses its polymerase via translational readthrough. The RNA signals involved in this readthrough process were characterized in vitro using a wheat germ extract translation system and in vivo via protoplast infections.
Newburn, Laura R., Nicholson, Beth L., Yosefi, Michael, Cimino, Peter A., White, K. Andrew   +4 more
openaire   +2 more sources

Poly(A)-Binding Protein Regulates the Efficiency of Translation Termination

Cell Reports, 2020
Summary: Multiple factors influence translation termination efficiency, including nonsense codon identity and immediate context. To determine whether the relative position of a nonsense codon within an open reading frame (ORF) influences termination ...
Chan Wu, Bijoyita Roy, Feng He, Kevin Yan, Allan Jacobson   +4 more
doaj   +1 more source

Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 system

EMBO Reports
Stop codon readthrough (SCR) is the process where translation continues beyond a stop codon on an mRNA. Here, we describe a strategy to enhance or induce SCR in a transcript-selective manner using a CRISPR-dCas13 system.
Lekha E. Manjunath, Anumeha Singh, Sangeetha Devi Kumar, Kirtana Vasu, D. Kar, Karthi Sellamuthu, S. Eswarappa   +6 more
semanticscholar   +1 more source

Genetic dissection reveals distinct contributions of the eS31 N‐terminal domain to translational accuracy in Saccharomyces cerevisiae

The FEBS Journal, EarlyView.
The eukaryote‐specific N‐terminal domain (NTD) of eS31 uses two distinct strategies to maintain translation fidelity. During elongation, a positively charged “hotspot” fine‐tunes the selection of incoming aa‐tRNA. During termination, the entire NTD acts as a structural scaffold to ensure the correct positioning of the release factor eRF1.
Qingxuan Gao, Hiroshi Otsuka, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D

Viruses, 2020
RNA elements in the untranslated regions of plus-strand RNA viruses can control a variety of viral processes including translation, replication, packaging, and subgenomic mRNA production.
Laura R. Newburn, Baodong Wu, K. Andrew White   +2 more
doaj   +1 more source

Who let the DoGs out? - biogenesis of stress-induced readthrough transcripts.

TIBS -Trends in Biochemical Sciences. Regular ed, 2021
Readthrough transcription caused by inefficient 3'-end cleavage of nascent mRNAs has emerged as a hallmark of the mammalian cellular stress response and results in the production of long noncoding RNAs known as downstream-of-gene (DoG)-containing ...
Nicolle A. Rosa-Mercado, J. Steitz
semanticscholar   +1 more source

Salt Stress Adaptations in Soybean Involve Alterations in Pre‐mRNA Processing

Plant, Cell &Environment, EarlyView.
ABSTRACT Salt stress can seriously affect plant survival. To adapt to salt stress, plants can alter gene expressions and/or pre‐mRNA processing patterns, or both. Previous studies could not comprehensively profile stress‐responsive pre‐mRNA processing patterns due to limitations in traditional sequencing technologies.
Shoudong Zhang, Zhixia Xiao, Ailin Liu, Dongpeng Ren, Shengjie Chen, Hanxue Zhang, Li Zhang, Zhili Wang, Jun Yang, Hon‐Ming Lam   +9 more
wiley   +1 more source

Computational analysis of stop codon readthrough in D.melanogaster [PDF]

Bioinformatics, 2003
Abstract Motivation: Readthrough is an unusual process in which a stop codon is misread or skipped. Recently it has been shown that some translation is regulated by the readthrough reactions although the complete mechanism is not clear.
Misaki, Sato, Hitomi, Umeki, Rintaro, Saito, Akio, Kanai, Masaru, Tomita   +4 more
openaire   +2 more sources