Results 11 to 20 of about 1,529,317 (170)

Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]

, 2011
Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els, Lievens, Antoon, Van Aelst, Stefan, Van den Bulcke, Marc   +3 more
core   +3 more sources

MicroRNA and histopathological characterization of pure mucinous breast carcinoma

Cancer Biology & Medicine, 2013
Objective: To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors. Methods: The data of 1,790 consecutive patients were retrospectively reviewed.
Fang Li, Ning Li, Bai-Hua Zhang, Da-Wei Wang, Ke-Lin Sun, Gui-Yu Chen, Ju-Wei Mu, You-Sheng Mao et al.   +7 more
doaj   +1 more source

Prevalence and geographic distribution of Babesia conradae and detection of Babesia vogeli in free-ranging California coyotes (Canis latrans)

International Journal for Parasitology: Parasites and Wildlife, 2022
Babesia species are intraerythrocytic piroplasms that can result in disease characterized by hemolytic anemia and thrombocytopenia. Of the 5 species that are known to infect canids in the United States, Babesia conradae is most frequently diagnosed in ...
Nadia N. Javeed, Laura Shultz, Samantha Barnum, Janet E. Foley, Emir Hodzic, Emily L. Pascoe, Beatríz Martínez-López, Niamh Quinn, Danielle Bucklin, Jonathan D. Dear   +9 more
doaj   +1 more source

Handheld real-time PCR device

Lab on a Chip, 2016
World's smallest, fully autonomous, handheld real-time PCR was shown in this contribution. The device can quickly process up to four samples at a time with detection capability of a single DNA copy. The fully integrated system includes all required electronics for fluorescence measurement, data viewing (LCD display) and processing, and is ideal for use
Ahrberg, Christian D., Ilic, Bojan Robert, Manz, Andreas, Neužil, Pavel   +3 more
openaire   +4 more sources

Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer-Evaluation of Several Markers with Real-Time Reverse Transcription-PCR [PDF]

, 2013
It is widely known that cells from epithelial tumors, e. g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic ...
Alexandra Kölbl, Alix-Panabieres, Balbin, Bergqvist, Bernard, Bolke, Braun, Braun, Chang, Christian Schindlbeck, De Cremoux, Dontu, Dydensborg, Fabre-Lafay, Fleming, Frasor, Gervasoni, Giani, Guo, Hedenfalk, Inaba, Jiang, Julia Neugebauer, Kim, Klaus Friese, Konecny, Krawczyk, Kurec, Lafleur, Livak, Markou, Matthias Ilmer, Mendoza, Mitas, Moliterni, Nikseresht, Noack, Pan, Pantel, Ring, Selvamurugan, Simone Hofmann, Slamon, Smerage, Solomayer, Stefan Hutter, Tai, Udo Jeschke, Ulrich Andergassen, Vandesompele, Verena Engelstädter, Wang, Weissenstein, Wu, Wu, Zhang   +55 more
core   +3 more sources

REAL TIME PCR;

The Professional Medical Journal, 2012
In recent years, real-time PCR has come forward as a robust and widely used molecular technique in clinical and biologicalsettings. Although it can detect very minute quantities of target nucleic acid, but quantification of specific nucleic acids is not an easy task.Accurate and precise quantification is hampered by a number of factors that may include
ABIDA RAZA, NAUREEN A KHATTAK
openaire   +3 more sources

Comparison of immunoassay and real-time PCR methods for the detection of Jembrana disease virus infection in Bali cattle [PDF]

, 2009
A sensitive diagnostic assay for the detection of infections with the bovine lentivirus Jembrana disease virus (JDV) is required in Indonesia to control the spread of Jembrana disease. Immunoassays are used routinely but are compromised by cross-reactive
Desport, M., Hartaningsih, N., Lewis, J.R., McNab, T., Tenaya, I.W., Wilcox, G.E.   +5 more
core   +2 more sources

Sensitive and specific detection of Trypanosoma cruzi DNA in clinical specimens using a multi-target real-time PCR approach [PDF]

, 2012
Background: The laboratory diagnosis of Chagas disease is challenging because the usefulness of different diagnostic tests will depend on the stage of the disease.
Aznar, Christine, da Silva, Alexandre J., Qvarnstrom, Yvonne, Schijman, Alejandro Gabriel, Steurer, Francis, Veron, Vincent   +5 more
core   +4 more sources

Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR [PDF]

, 2017
Background Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses ...
Agoti, Charles N., Bett, Anne, Cane, Patricia A., Kamau, Everlyn, Lewa, Clement S., Nokes, D. James, Oketch, John, Otieno, Grieven P., Owor, Betty E.   +8 more
core   +2 more sources

Serology versus Real Time PCR in the Diagnosis of Human Brucellosis

Journal of Pure and Applied Microbiology, 2017
With the unwelcome re-emergence of brucellosis in different regions of the world, an accurate and timely diagnosis of this zoonosis has become a daunting challenge.
Sara El-Hossain Aly Reda, Mona A. Abd El-Messiah, Lamiaa A. Madkour, Abd El- Meguid Kassem   +3 more
doaj   +1 more source