Results 91 to 100 of about 644,089 (298)

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source

DNA extraction and polymerase chain reaction

Journal of Cytology, 2019
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
doaj   +1 more source

Transgenes monitoring in an industrial soybean oil processing by conventional and real-time polymerase chain reaction [PDF]

, 2009
In recent years a great effort has been devoted to the development of new methods for the qualitative and quantitative detection of transgenic sequences in food.
Amaral, Joana S., Costa, J., Mafra, I., Oliveira, Beatriz   +3 more
core  

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]

, 2019
Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A., Caruso A. G., Davino S., Panno S., Ruiz-Ruiz S., San Ambrosio M. I. F.   +5 more
core   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Herpes simplex keratitis: Challenges in diagnosis and clinical management [PDF]

, 2017
Herpes simplex virus is responsible for numerous ocular diseases, the most common of which is herpetic stromal keratitis. This is a recurrent infection of the cornea that typically begins with a subclinical infection of the cornea that establishes a ...
Azher, Tayaba N, Huang, Andrew JW, Stuart, Patrick M, Tajfirouz, Deena, Yin, Xiao-Tang   +4 more
core   +3 more sources

CX3CL1 in Early Detection of Alzheimer's Disease: Plasma Dynamics Across Age and Disease Stages

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Backgrounds Alzheimer's disease (AD) is characterized by amyloid‐beta plaques, tau tangles, and neuroinflammation. C‐X3‐C motif chemokine ligand 1 (CX3CL1, also known as fractalkine), a neuroimmune chemokine implicated in AD pathogenesis, shows inconsistent alterations in plasma/serum across studies.
Ling Wang, Yujie Liu, Fei Li, Xuelin Li, Lanlan Li, Jie Zhang, Yali Xu   +6 more
wiley   +1 more source

A comparison of three diagnostic platforms for the detection of influenza A and B in children

Biomedical and Biotechnology Research Journal, 2018
Background: Viral flu is the predominant cause of hospitalization in young children, which invariably leads to enhanced morbidity and mortality in children in developing countries. Initial treatment of viral flu is based on presumptive diagnosis. Bedside
Furqan Kabir, Marvi Tariq, Fatima Aziz, Syed Imran Rizvi, Shahida Qureshi, Asad Ali, Najeeha Talat Iqbal   +6 more
doaj   +1 more source

Mechanically Tunable Bone Scaffolds: In Vivo Hardening of 3D‐Printed Calcium Phosphate/Polycaprolactone Inks

Advanced Functional Materials, EarlyView.
A 3D bone scaffold with osteogenic properties and capable of hardening in vivo is developed. The scaffold is implanted in a ductile state, and a phase transformation of the ceramic induces the stiffening and strengthening of the scaffold in vivo. Abstract Calcium phosphate 3D printing has revolutionized customized bone grafting.
Miguel Mateu‐Sanz, Pablo Varela, Laura del‐Mazo‐Barbara, Irene Lodoso‐Torrecilla, Emilio Jiménez‐Piqué, Jordi Franch, Miguel Alaminos, Maria‐Pau Ginebra   +7 more
wiley   +1 more source

Validating Real-Time Polymerase Chain Reaction (PCR) Assays

, 2021
For many years, the development of assays took place in the laboratories where the test was required. Whilst more and more commercially developed tests have become available, novel assays continue to be developed in academic hospital laboratories. Although commercial kits are more expensive than LDTs, they enable the rapid introduction of assays, with ...
openaire   +1 more source