Results 81 to 90 of about 731,111 (296)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Detection of Porcine DNA in Processed Beef Products Using Real Time – Polymerase Chain Reaction
Indonesian Journal of Halal Research, 2019 Meat is one of food materials which has protein source and mostlyconsumed by non-vegetarian. Consuming halal food is an obligation for every Muslim. Meat processed products usually contaminated by pork.
Triayu Septiani
doaj +1 more source
Herpes simplex keratitis: Challenges in diagnosis and clinical management [PDF]
, 2017 Herpes simplex virus is responsible for numerous ocular diseases, the most common of which is herpetic stromal keratitis. This is a recurrent infection of the cornea that typically begins with a subclinical infection of the cornea that establishes a ...
Azher, Tayaba N +4 more
core +3 more sources
FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Performance of Robust Regression Methods in Real-Time Polymerase Chain Reaction Calibration
The International Journal of Biological Markers, 2014 The ordinary least squares (OLS) method is routinely used to estimate the unknown concentration of nucleic acids in a given solution by means of calibration.
Annalisa Orenti, Ettore Marubini
doaj +1 more source
Real-Time Polymerase chain reaction trends in COVID-19 patients
Pakistan Journal of Medical Sciences, 2020 Objective: To assess trends of real-time Polymerase Chain Reaction test in Coronavirus infected Patients. Methods: This cross-sectional analytical study was conducted at Tertiary Care Institute, Rawalpindi from March 2020 to June 2020. All patients confirmed COVID positive by real-time Polymerase Chain Reaction (PCR) with recent travel history ...
Abbas, Sana +3 more
openaire +3 more sources
FEBS Open Bio, EarlyView.5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley +1 more source
Development of a real time polymerase chain reaction for quantitation of Schistosoma mansoni DNA
Memorias do Instituto Oswaldo Cruz, 2006 This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument.
Ana Lisa do Vale Gomes +3 more
doaj +1 more source
Prevalence of Clarithromycin-Resistant Helicobacter pylori in Patients With Chronic Tonsillitis by Allele-Specific Scorpion Real-Time Polymerase Chain Reaction Assay [PDF]
, 2013 Objectives/Hypothesis: To investigate the allelic prevalence of resistance to clarithromycin in the DNA of clinical isolates of Helicobacter pylori obtained from biopsy specimens of patients with chronic tonsillitis by Scorpion real-time polymerase ...
Johari, Pouran +2 more
core
Detection of medically important Candida species by absolute quantitation real-time polymerase chain reaction [PDF]
, 2014 Background: Chronic obstructive pulmonary disease (COPD) is one of the most important causes of disability and mortality in the world. Although cigarette smoking and environmental pollutants have been recognized as the major causes of COPD, the role of ...
Chong, Pei Pei +3 more
core +2 more sources