Results 151 to 160 of about 644,089 (298)

Quantification Strategies in Real-time Polymerase Chain Reaction [PDF]

, 2019
David Rodríguez-Lázaro, Marta Hernández   +1 more
  +4 more sources

Kidney Organoids in Drug Development: Integrating Technological Advances and Standardization for Effective Implementation

Advanced Healthcare Materials, EarlyView.
This review examines how emerging enabling technologies enhance the physiological relevance, scalability, and reproducibility of kidney organoids, while advanced analytical approaches support model validation and deepen mechanistic insight into nephrotoxicity.
Helen Kearney, Silvia M. Mihăilă, Lorenzo Moroni, Carlos Mota   +3 more
wiley   +1 more source

A Tri‐Culture Heart‐on‐a‐Chip Platform With iPSC‐Derived Cardiac Cells for Predictive Cardiotoxicity Testing

Advanced Healthcare Materials, EarlyView.
This study presents the first entirely isogenic heart‐on‐chip, unifying cardiomyocytes, fibroblasts, and endothelial cells from a single iPSC source. The platform reveals a critical biological insight: the endothelium actively shields cardiac tissue from drug‐induced toxicity, challenging the predictive accuracy of conventional, avascular models for ...
Karine Tadevosyan, Jose Yeste, Mar Alvarez, Denise Marrero, Laura Casado‐Medina, Yvonne Richaud‐Patin, Olalla Iglesias García, Jagoda Litowczenko‐Cybulska, Alba Morillas Garcia, Anton Guimerà, Xavi Illa, Rosa Villa, Angel Raya   +12 more
wiley   +1 more source

Real-time polymerase chain reaction (PCR) for <i>Pneumocystis jirovecii</i> detection in lower respiratory tract samples. [PDF]

Microbiol Spectr
Giardina FAM, Ramus M, Campanini F, Lilleri D, Ferretti V, Pitrolo AMG, Vola A, Maserati R, Baldanti F, Paolucci S.   +9 more
europepmc   +1 more source

Zinc‐Containing Bioactive Glass Programs Macrophage Polarization through Extracellular Traps Regulation for Enhanced Diabetic Wound Healing

Advanced Healthcare Materials, EarlyView.
Zinc‐containing bioactive glass (ZnBG) promotes diabetic wound healing by regulating macrophage extracellular traps (METs). Specifically, ZnBG reduces oxidative stress and inhibits the PAD4 and NLRP3/caspase‐1/GSDMD signaling pathways, thereby suppressing MET formation.
RuiYang Sun, XueBo Wei, ZhuoYang Song, JunJun Luo, JingYi Ye, YinNan Zhang, LiFei Zhu, Jie Gao, Hongyu Zhang, Hong Zhu, Xin Wang, Ke Xu   +11 more
wiley   +1 more source

Selection and evaluation of reference genes for quantitative real-time polymerase chain reaction normalization in Pieris melete (Lepidoptera, Pieridae). [PDF]

J Insect Sci
Jiang T, Liu X, Zou N, Zhang W, Peng Y, Xiao H.   +5 more
europepmc   +1 more source

Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy

Advanced Materials, EarlyView.
Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa, Gracia Mendoza, Silvia Irusta, Manuel Arruebo   +3 more
wiley   +1 more source

A case of herpes simplex keratouveitis diagnosed by real time polymerase chain reaction

Indian Journal of Ophthalmology, 2015
Ankush Gondchawar, Jyotirmay Biswas, Sowmiya Murali, Lavanya Mayura Priya Eawaran   +3 more
doaj   +1 more source

Syndromic Testing-The Evaluation of Four Novel Multiplex Real-Time Polymerase Chain Reaction Panels. [PDF]

Diagnostics (Basel)
Yılmaz M, Kilic S, Bayrakdar F, Ötgün SN, Tosun AI, Zeybek U, Çelik F, Aygun G, Safak B, Mahroum N.   +9 more
europepmc   +1 more source

High‐Throughput In Vivo Screening Using Barcoded mRNA Identifies Lipid Nanoparticles With Extrahepatic Tropism for In Situ Immunoengineering

Advanced Materials, EarlyView.
A high‐throughput in vivo mRNA LNP screening platform is developed and employed to screen a large library of 122 mRNA LNPs in vivo for delivery to immune, stromal, and parenchymal cells, identifying promising LNP candidates. A novel small particle flow cytometry‐based protein adsorption analysis method is utilized to interrogate protein corona ...
Alex G. Hamilton, Ajay S. Thatte, Junchao Xu, Zhangyi Luo, Hannah C. Safford, Kelsey L. Swingle, Jenna Muscat‐Rivera, Michael Kegel, Xuexiang Han, Ryann A. Joseph, Amanda M. Murray, Hannah C. Geisler, Ricardo C. Whitaker, Lulu Xue, Roman Spektor, Jilian R. Melamed, Drew Weissman, Michael J. Mitchell   +17 more
wiley   +1 more source