Results 241 to 250 of about 644,089 (298)

Evaluation of Mir-1290 as a Possible Diagnostic Factor in the Serum of Oral Squamous Cell Carcinoma Patients with Qualitative Real-Time Polymerase Chain Reaction. [PDF]

Iran J Pathol
Bagheri Shirvan S, Shahabinejad M, Mohajertehran F, Nazari N.   +3 more
europepmc   +1 more source

Quantification of Porphyromonas gingivalis using real-time polymerase chain reaction in subjects suffering from chronic periodontitis with and without rheumatoid arthritis.

J Indian Soc Periodontol
Bhatsange A, Rajput K.
europepmc   +1 more source

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Real‐Time Polymerase Chain Reaction

ChemBioChem, 2003
AbstractReal‐time PCR is the state‐of‐the‐art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real‐time PCR machines of different design is continuously increasing.
Jochen, Wilhelm, Alfred, Pingoud
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The real-time polymerase chain reaction

Molecular Aspects of Medicine, 2006
The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR [Bustin, S.A., 2004. A-Z of Quantitative PCR. IUL Press, San Diego, CA]. This new technique is a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the ...
Mikael, Kubista, José Manuel, Andrade, Martin, Bengtsson, Amin, Forootan, Jiri, Jonák, Kristina, Lind, Radek, Sindelka, Robert, Sjöback, Björn, Sjögreen, Linda, Strömbom, Anders, Ståhlberg, Neven, Zoric   +11 more
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Electrochemical Real-Time Polymerase Chain Reaction

Journal of the American Chemical Society, 2006
In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon the advantages of the well-established fluorescence-based counterpart, such as short assay time (simultaneous target DNA amplification and detection).
Yeung, Siu Wai, Lee, Ming Hung Thomas, Hsing, I-Ming   +2 more
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Real-Time Polymerase Chain Reaction Detection Methods

Recent Patents on DNA & Gene Sequences, 2008
Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification.
Douglas F, Whitman, Sherry A, Dunbar
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Robust regression methods for real-time polymerase chain reaction

Analytical Biochemistry, 2015
Current real-time polymerase chain reaction (PCR) data analysis methods implement linear least squares regression methods for primer efficiency estimation based on standard curve dilution series. This method is sensitive to outliers that distort the outcome and are often ignored or removed by the end user.
Trypsteen, Wim, De Neve, Jan, Bosman, Kobus, Nijhuis, Monique, Thas, Olivier, Vandekerckhove, Linos, De Spiegelaere, Ward   +6 more
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Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

2010
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Hongxin, Fan, Ryan S, Robetorye
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