Results 101 to 110 of about 22,071,005 (304)

Desenvolvimento de iniciadores e sondas para detecção de cry1A.105 e cry2Ab2 e aplicação de PCR e PCR em tempo real para detecção de OGM em alimentos [PDF]

, 2011
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Ciência dos Alimentos, Florianópolis, 2011Reação em Cadeia da Polimerase (PCR) e PCR quantitativa em tempo real são usadas para detecção ...
Dinon, Andréia Zilio
core  

Primer sequences for real-time quantitative PCR.

, 2015
Primer sequences for real-time quantitative PCR.
Yuanyuan Qian (704794), Melissa Skibba (494842), Peng Zhong (106399), Dandan Liang (605312), Xiaokun Li (166455), Chunlai Zeng (704795), Lianpin Wu (704797), Guang Liang (206236), Zheng Xu (162992), Tiemin Wei (704796)   +9 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus

Frontiers in Veterinary Science
IntroductionThe African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques.
Silvia Dei Giudici, Piero Bonelli, Maria Giovanna Tilocca, Simona Cau, Pier Paolo Angioi, Anna Maria Sechi, Bruna Vodret, Annalisa Oggiano   +7 more
doaj   +1 more source

Quantitative Detection of t(14;18)-Positive Cells by Real-Time Quantitative PCR Using Fluorogenic Probes

BioTechniques, 1998
To detect t(14;18)-positive cells present in human lymphoma tissue, bone marrow aspirates and peripheral blood mononuclear cells (PBMNC), we have established an automated, real-time quantitative PCR using double-labeled fluorogenic probes. In relation to
Dölken Lars, Schüler Frank, Dölken Gottfried   +2 more
doaj   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Primers used for real-time quantitative PCR.

, 2013
Primers used for real-time quantitative PCR.
Jennifer L. Sargent (87901), Minghua Wu (129689), Jun Wei (208481), Michael L. Whitfield (87904), Asish K. Ghosh (360107), Carol Feghali-Bostwick (270228), Kazuhiro Komura (360109), Qi-Quan Huang (217194), Manu Jain (263718), John Varga (38678)   +9 more
core   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

Virology Journal, 2018
Background Human adenovirus (HAdV) serotypes 2, 3 and 7 are more prevalent than other serotypes and have been associated with severe pneumonia in pediatric children.
Fang-zhou Qiu, Xin-xin Shen, Meng-chuan Zhao, Li Zhao, Su-xia Duan, Chen Chen, Ju-Ju Qi, Gui-xia Li, Le Wang, Zhi-shan Feng, Xue-jun Ma   +10 more
doaj   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source