Results 101 to 110 of about 21,194,347 (300)

Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus

Frontiers in Veterinary Science
IntroductionThe African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques.
Silvia Dei Giudici, Piero Bonelli, Maria Giovanna Tilocca, Simona Cau, Pier Paolo Angioi, Anna Maria Sechi, Bruna Vodret, Annalisa Oggiano   +7 more
doaj   +1 more source

[Real time quantitative PCR].

Experimental & molecular medicine, 2002
So far, quantitative techniques, such as PCR and FISH, have been used to detect of DNA and RNA. However, it is difficult to measure and compare the exact amount of amplified products with the results of endpoint analysis in conventional PCR techniques.
openaire   +1 more source

Detection of Mycosphaerella graminicola in Wheat Leaves by a Microsatellite Dinucleotide Specific-Primer [PDF]

, 2011
Early detection of infection is very important for efficient management of Mycosphaerella graminicola leaf blotch. To monitor and quantify the occurrence of this fungus during the growing season, a diagnostic method based on real-time PCR was developed ...
Bahkali, A.H., Verreet, J.A., Abd-Elsalam, K., Moslem, M., Mohamed Moslem, Ali H. Bahkali, Wit, P.J.G.M., de, Pierre J. G. M. De Wit, Kamel Abd-Elsalam, Joseph-Alexander Verreet   +9 more
core   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

Journal of Animal Science and Biotechnology, 2012
MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation.
Kang Kang, Peng Xiao, Luo Jun, Gou Deming   +3 more
doaj   +1 more source

HUMAN HERPESVIRUS 8 UPREGULATES ACTIVATING TRANSCRIPTION FACTOR 4 (ATF4) and REAL TIME PCR TO ASSESS TOTAL BACTERIAL LOAD IN CHRONIC WOUNDS. [PDF]

, 2010
I. Human Herpesvirus 8 Upregulates Activator Transcription Factor 4 BACKGROUND: Human herpesvirus 8 (HHV-8) is the primary etiologic agent of Kaposi’s sarcoma, a highly vascularised neoplasm of endothelial origin characterized by inflammation ...
Gentili, Valentina
core  

Quantitative real-time RT-PCR.

, 2013
Expression of (A) AR and PSA and (B) c-jun, KLK2, NKX3.1 and c-Myc mRNA levels in response to Doc or Pac was evaluated by quantitative real time PCR (qPCR).
Binshen Chen (481747), Martina Tinzl (481746), Julius Semenas (481749), Shao-Yong Chen (481748), Per-Anders Abrahamsson (481750), Nishtman Dizeyi (443546)   +5 more
core   +1 more source

Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel Internal Amplification Control approach using Biotrove OpenArrays [PDF]

, 2009
PCR-based detection assays are prone to inhibition by substances present in environmental samples, thereby potentially leading to inaccurate target quantification or false-negative results.
Gent-Pelzer, M. van, Gent-Pelzer, M.P.E., van, Van Doorn, R., Doorn, R. van, Speksnijder, A., Speksnijder, A.G.C.L., Klerks, M.M., Schoen, C.D., van Gent-Pelzer, M., Klerks, M., Gent-Pelzer van, M., Doorn, R., van, Kowalchuk, G.A., van Gent-Pelzer, M.P.E.   +13 more
core   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Detecção de Listeria monocytogenes em produtos a base de pescado e comparação de métodos PCR em tempo real e ISO11290-1 [PDF]

, 2015
Dissertação (mestrrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Ciência dos Alimentos, Florianópolis, 2015.O pescado é um alimento de extrema importância na dieta dos seres humanos, pois possui ...
Barretta, Clarissa
core