Results 111 to 120 of about 21,194,347 (300)

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Quantitative real-time PCR oligonucleotides.

, 2016
Quantitative real-time PCR oligonucleotides.
Raquel Fucho (85556), Ester García-Casarrubios (3150912), María Jesús Obregón (3150906), Ángela M. Valverde (3150909), David Sebastián (3150915), Minéia Weber (3150921), Joan F. Mir (3150924), Laura Herrero (562951), Antonio Zorzano (35886), María Calderon-Dominguez (3150918), Dolors Serra (562952)   +10 more
core   +1 more source

Prevalence of bloodstream pathogens is higher in neonatal encephalopathy cases vs. controls using a novel panel of real-time PCR assays. [PDF]

, 2014
BACKGROUND: In neonatal encephalopathy (NE), infectious co-morbidity is difficult to diagnose accurately, but may increase the vulnerability of the developing brain to hypoxia-ischemia.
Klein Nigel, Hughes Peter, Willey Barbara A., Nicola J Robertson, Kurinczuk, JJ, Kurinczuk, Jennifer, Barbara A Willey, Alison M Elliott, Tann Cally J., Were Jackson, Were, J, Elliott, Alison M, Tann, CJ, Robertson, NJ, Peter Hughes, Robertson Nicola J., Jennifer J Kurinczuk, Nkurunziza, Peter, Margaret Nakakeeto, Oweka, James, Klein, Nigel, Were, Jackson, Tann, Cally J, Willey, Barbara A, Hughes, Peter, Robertson, Nicola J, Harris, KA, Nakakeeto, M, Natasha Nyombi, Kurinczuk, Jennifer J, Nakakeeto Margaret, Elliott Alison M., Cally J Tann, Harris, Kathryn A, Nkurunziza Peter, Nyombi, Natasha, Oweka James, Elliott, AM, Nigel Klein, Harris Kathryn A., Kathryn A Harris, Nkurunziza, P, Willey, BA, Nyombi Natasha, Hughes, P, Oweka, J, Jackson Were, Nakakeeto, Margaret, James Oweka, Nyombi, N, Klein, N, Kurinczuk Jennifer J., Peter Nkurunziza   +52 more
core   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Real-time quantitative RT-PCR.

, 2012
Real-time quantitative RT-PCR.
Hongjie Fan (167764), Zhe Ma (167751), Li Yi (167757), Chengping Lu (167772), Hui Zhang (7197)   +4 more
core   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Real-time quantitative PCR primers.

, 2015
Real-time quantitative PCR primers.
Xiao-Qiu Zhou (387187), Lin Feng (84785), Ling Tang (387186), Wei-Dan Jiang (387183), Sheng-Yao Kuang (387184), Yong-An Zhang (829491), Juan Zhao (109151), Hua-Fu Zhao (829489), Yang Liu (4829), Jun Jiang (149215), Pei Wu (327833), Wu-Neng Tang (829490)   +11 more
core   +1 more source