Results 151 to 160 of about 1,064,076 (204)

Real-time quantitative PCR in parasitology

Trends in Parasitology, 2002
Standard techniques for counting parasites are often time-consuming, difficult and inaccurate, and occasionally unpleasant. Real-time quantitative polymerase chain reaction has recently been applied to parasitology, specifically Plasmodium, Toxoplasma, Leishmania and Neospora.
Andrew, Bell, Lisa, Ranford-Cartwright
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Quantitative Real-Time PCR: Recent Advances

2016
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or ...
Charanjeet, Singh, Sinchita, Roy-Chowdhuri   +1 more
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Accuracy of quantitative real-time PCR analysis

Doklady Biochemistry and Biophysics, 2013
When solving many problems in biology and med icine, it is necessary to detect the presence of specific nucleic acid sequences in samples. For this purpose, polymerase chain reaction (PCR)—a chain enzy matic reaction of multiplication of the analyzed DNA fragment, realized in the form of successive rounds of duplication of fragments present in the ...
D G, Sochivko, A A, Fedorov, D A, Varlamov, V E, Kurochkin, R V, Petrov   +4 more
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High‐Throughput Quantitative Real‐Time PCR

Current Protocols in Human Genetics, 2008
AbstractRecent technical advances in quantitative real‐time PCR (qRT‐PCR) have allowed for extensive miniaturization, thereby rendering the technique amenable to high‐throughput assays. Large numbers of different nucleic acids can now rapidly be measured quantitatively. Many investigations can benefit from this approach, including determination of gene
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Basic principles of real-time quantitative PCR

Expert Review of Molecular Diagnostics, 2005
Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications.
Manit, Arya, Iqbal S, Shergill, Magali, Williamson, Lyndon, Gommersall, Neehar, Arya, Hitendra R H, Patel   +5 more
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Real-Time Quantitative PCR of Telomere Length

Molecular Biotechnology, 2004
Telomeres cap the ends of chromosomes and are essential for the protection of chromosomes, as well as restricting the replicative potential of a cell. These functions are achieved by the regulation of telomeric repeat length, making the measurement of telomere length a useful aid in the elucidation of the replicative history and potential of cells ...
Marcel E, Gil, Thérèsa L, Coetzer
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Quantitative Real-Time RT-PCR

2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA, NARDON, ERMANNO, PRACELLA, DANAE, BONIN, Serena   +3 more
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Multiplex Analyses Using Real-Time Quantitative PCR

2016
Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene expression in real time. Multiplex qPCR requires the use of probe-based assays, in which each probe is labeled with a unique fluorescent dye, resulting in different observed colors for each assay.
Steve F C, Hawkins, Paul C, Guest
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Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
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