In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-
Ana Carolina Basílio Palmieri +3 more
doaj
Schematic illustration of the proposed mechanism: PEG/RGD‐PSLs mimic apoptotic cells to engage PS receptors (notably CD300a), transducing an inhibitory signal that suppresses the MyD88/NF‐κB pathway, leading to global anti‐inflammatory and pro‐reparative effects.
Lele Wu +10 more
wiley +1 more source
Development and epidemiological investigation of a TaqMan-based multiplex real-time quantitative PCR assay for simultaneous detection of five bovine viruses (BVDV, AKAV, BNoV, BEV, and BCoV). [PDF]
Zhang H +9 more
europepmc +1 more source
Development of a Real-Time Quantitative PCR Assay for the Specific Detection of Bacillus velezensis and Its Application in the Study of Colonization Ability. [PDF]
Xu S, Xie X, Shi Y, Chai A, Li B, Li L.
europepmc +1 more source
Diabetic wound healing is hindered by a pathological crosstalk wherein elevated IL‐11 induces M1 macrophage polarization, leading to IL‐1β/IL‐6 release and subsequent fibroblast senescence via RAS/p38MAPK/p53/E2F1 signaling. Here, we develop a Gel@M‐Rho microneedle patch that delivers rhoifolin‐loaded, macrophage membrane‐coated nanoparticles to target
Jiewen Liao +10 more
wiley +1 more source
Development and evaluation of single-plex TaqMan real-time quantitative PCR assays for the detection of six tick-borne pathogenic viruses in northeastern China. [PDF]
Xu S +7 more
europepmc +1 more source
Establishment of a reverse transcription real-time quantitative PCR method for Getah virus detection and its application for epidemiological investigation in Shandong, China. [PDF]
Cao X +11 more
europepmc +1 more source
A water‐activated phospholipid phase‐separation gel is repurposed as a drug‐free surgical barrier for postoperative adhesion prevention. Propylene glycol promotes rapid lamellar gel reinforcement, prolonged local retention, and tissue coverage, while phosphatidylcholine‐mediated immunomodulation suppresses inflammatory and fibrotic responses. This dual‐
Jong‐Ju Lee +6 more
wiley +1 more source
Maternal vaginal colonization screening for term singleton pregnancy: comparative evaluation of metagenomic next-generation sequencing (mNGS) versus real-time quantitative PCR (qPCR). [PDF]
Zhang J, Liang J, Lv F, Guo Z.
europepmc +1 more source
Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri. [PDF]
Verbrugghe P +3 more
europepmc +1 more source

