Results 151 to 160 of about 21,194,347 (300)

Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR. [PDF]

Life (Basel), 2022
Kanayev D, Abilmazhenova D, Akhmetollayev I, Sekenova A, Ogay V, Kulyyassov A.   +5 more
europepmc   +1 more source

A Magneto‐Mechanically Activated Nerve Guidance Conduit Promotes Peripheral Nerve Regeneration via TIMP1‐Mediated Membrane Tension Transfer

Advanced Functional Materials, EarlyView.
This study presents a magneto‐mechanical strategy that incorporates FP@MSCs into an aligned PCL/GelMA nerve guidance conduit. Magnetic stimulation increases membrane tension in FP@MSCs, triggering cytoskeletal remodeling, Schwann cell‐like differentiation, and TIMP1 secretion. TIMP1 activates ITGB1/CD63–FAK signaling in NE‐4C cells, increasing membrane
Xinyu Zhu, Yumin Wu, Xiang Gao, Tianlu Wang, Wanting Jia, Xueying Mei, Huanzhong Ji, Zongxi Wu, Yujie Liang, Xiang Li, Kan Li, Jingyuan Li, Guangsen Zheng, Guiqing Liao, Siyong Gao   +14 more
wiley   +1 more source

Comparing the broth enrichment-multiplex lateral flow immunochromatographic assay with real time quantitative PCR for the rapid detection of carbapenemase-producing organisms in rectal swabs. [PDF]

BMC Infect Dis, 2023
Wang Y, Song H, Xu M, Li D, Ran X, Sun Z, Chen Z.   +6 more
europepmc   +1 more source

Nano Borophene as a Programmable 2D Scaffold for Organized DNA Assembly and Long‐Range Energy Transfer

Advanced Functional Materials, EarlyView.
Borophene is introduced as a programmable 2D bio‐interface that organizes DNA through dual boron–sulfur and nucleobase interactions. This molecular architecture enables efficient long‐range nanosurface energy transfer, establishing new design principles for functional nano–bio interfaces and amplification‐free optical biosensing.
Teresa Aditya, Pranay Saha, John M. Sansalone, Shraddha Krishnakumar, Matthew Molinaro, André J. van der Vlies, Nada Maher, Adrienne Griffiths, Zakhro Zakirova, Ketan Dighe, Narayana R. Aluru, Dipanjan Pan   +11 more
wiley   +1 more source

Patient‐Derived 3D Heart‐On‐a‐Chip Model of Dilated Cardiomyopathy With Embedded Bead‐Based Mapping of Tissue Contractility

Advanced Healthcare Materials, EarlyView.
A heart‐on‐a‐chip model of dilated cardiomyopathy is developed from patient‐derived induced pluripotent stem cells. The model recapitulates key disease phenotypes and enables functional assessment through integrated bead‐based tracking and pillar deflection measurements.
Ali Mousavi, Ludovic Mouttet, Shihao Cui, Yasaman Hekmatnia, Mehran Mottahedi, Ida Derish, Naimeh Rafatian, Mark Aurousseau, Gregor Andelfinger, Renzo Cecere, Houman Savoji   +10 more
wiley   +1 more source

Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Tissues from Bumble Bees (Bombus Terrestris) of Different Lines. [PDF]

Int J Mol Sci, 2022
Sankar K, Yoon HJ, Lee YB, Lee KY.
europepmc   +1 more source

How is Nanoarchitectonics Shaping Extracellular Vesicle Research?

Advanced Healthcare Materials, EarlyView.
The convergence of nanoarchitectonics and extracellular vesicle biology is reshaping next‐generation nanomedicine by enabling rationally designed nanostructures for enhanced EVs detection, engineering, and therapy. ABSTRACT Extracellular vesicles (EVs) have gained recognition as crucial mediators of cell‐to‐cell communication, holding immense potential
Madushani Dahanayake, Md Shahriar A. Hossain, Chia‐Hung Liu, Carlos Salomon, Yusuke Yamauchi, Mostafa Kamal Masud   +5 more
wiley   +1 more source

Establishment of a TaqMan-based real-time quantitative PCR method for detection of exogenous fowl adenovirus type Ⅰ, type Ⅲ and avian leukosis virus in human cold adapted live attenuated influenza vaccines. [PDF]

Heliyon, 2023
Wu M, Zhi B, Sheng R, Zhou E, Zhou Q, Li J, Fu X, Wu Y, Zhang Y, Sun P, Jia W, Shi L, Liu D, Zhao H, Su W.   +14 more
europepmc   +1 more source

Spatially Controlled Embedded Bioprinting of Prevascularized Spheroids‐Containing Biopatch Enables Functional Skin Regeneration

Advanced Healthcare Materials, EarlyView.
A spatially controlled embedded bioprinting strategy enables precise engineering of prevascularized multicellular spheroids within skin‐derived dECM bioinks. Optimized spheroid size, composition, and spacing promote vascular maturation, paracrine signaling, and tissue integration, resulting in enhanced angiogenesis, blood perfusion, and functional skin
Minjun Ahn, Gyu Tae Park, Dongjun Lee, Sik Yoon, Sae‐Ock Oh, Jae Ho Kim, Byoung Soo Kim   +6 more
wiley   +1 more source

Reference Gene U2 Enables Direct Comparison between Relative Gene Expression Levels of Vascular Smooth Muscle Cells in Tissue and Culture Using Real-Time Quantitative PCR. [PDF]

Cells, 2023
Rager C, Klöpper T, Pfeil U, Tasch S, Whittaker MR, Exintaris B, Mietens A, Middendorff R.   +7 more
europepmc   +1 more source