Results 111 to 120 of about 1,220,561 (394)

Primers for real-time quantitative RT-PCR.

, 2022
Primers were designed using Primer-BLAST or according to previous reports[24,62]. 24. Sengiku, A.; Ueda, M.; Kono, J.; Sano, T.; Nishikawa, N.; Kunisue, S.; Tsujihana, K.; Liou, L.S.; Kanematsu, A.; Shimba, S., et al. Circadian coordination of ATP release in the urothelium via connexin43 hemichannels. Sci Rep 2018, 8, 1996, doi:10.1038/s41598-018-20379-
openaire   +2 more sources

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Real-time RT-PCR v1

, 2017

openaire   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process [PDF]

, 2008
12 pages, 2 figures, 4 tables.-- PMID: 19102748 [PubMed].-- PMCID: PMC2629474.[Background] The elucidation of gene expression patterns leads to a better understanding of biological processes.
Marino Expósito-Rodríguez, Andrés A Borges, Andrés Borges-Pérez, José A Pérez   +3 more
core   +2 more sources

An assessment of real-time RT-PCR kits for SARS-CoV-2 detection.

Japanese journal of infectious diseases (Print), 2020
The COVID-19 outbreak caused by SARS-CoV-2 in Wuhan (China) in December 2019 is currently spreading rapidly and globally. We recently reported a laboratory diagnostic protocol for SARS-CoV-2 based on real-time RT-PCR assays using two primer sets, N and ...
Kiyoko Okamaoto, K. Shirato, NagaNaganori Nao, Shinji Saito, T. Kageyama, H. Hasegawa, Tadaki Suzuki, S. Matsuyama, M. Takeda   +8 more
semanticscholar   +1 more source

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Virus culture and real-time RT-PCR in identifying influenza viruses from influenza-like illness cases in Indonesia 2007-2008

Health Science Journal of Indonesia, 2012
Latar belakang: Adanya perbedaan hasil antara kultur virus dengan real-time polymerase chain reaction (RT-PCR) yang digunakan dalam surveilans influenza-like illness (ILI) menunjukkan perlunya mengevaluasi hasil kultur virus yang didapatkan dengan hasil ...
Irene L. Indalao, Vivi Setiawaty, Hana A. Pawestri, Subangkit Subangkit   +3 more
doaj   +3 more sources

A Pan-Lyssavirus Taqman Real-Time RT-PCR Assay for the Detection of Highly Variable Rabies virus and Other Lyssaviruses

PLoS Neglected Tropical Diseases, 2017
Rabies, resulting from infection by Rabies virus (RABV) and related lyssaviruses, is one of the most deadly zoonotic diseases and is responsible for up to 70,000 estimated human deaths worldwide each year.
A. Wadhwa, Kimberly Wilkins, Jin-xin Gao, R. E. Condori Condori, C. Gigante, Hui Zhao, Xiaoyue Ma, J. Ellison, L. Greenberg, A. Velasco-Villa, L. Orciari, Yu Li   +11 more
semanticscholar   +1 more source

Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus

China CDC Weekly, 2020
What is already known on this topic? A novel human coronavirus, known as SARS-CoV-2 or 2019-nCoV, is the causative agent of the coronavirus disease 2019 (COVID-19).
P. Niu, R. Lu, Li Zhao, Huijuan Wang, Baoying Huang, F. Ye, Wenling Wang, W. Tan   +7 more
semanticscholar   +1 more source