Results 131 to 140 of about 19,862,753 (307)

Microgels Enable iPSCs to Assemble, Expand, and Differentiate Into Organoids—From Sizable to High‐Throughput

open access: yesAdvanced Materials, EarlyView.
A chemically defined PEG‐based microgel platform enables scalable, reproducible production of three‐dimensional microgel‐iPSC constructs in multiple sizes. The system is compatible with high‐throughput automation and supports human iPSC expansion and differentiation within a single construct, including directed differentiation into cardiac organoids ...
Laura Klasen   +8 more
wiley   +1 more source

Primers for quantitative real-time RT-PCR.

open access: yes, 2013
Primers for quantitative real-time RT-PCR.
Nobuhiko Wada (430568)   +10 more
core   +1 more source

A Retinal Matrix Stiffening Model Reveals FAK‐Dependent Mechanotransduction in Pericytes Underlies Vascular Destabilization

open access: yesAdvanced Materials, EarlyView.
In this study, we show that retinal matrix stiffness is increased in diabetic mice. Using engineered human vascular models with controlled matrix stiffness, we demonstrate that stiffening activates focal adhesion kinase (FAK) signaling in pericytes, suppresses Notch signaling, and disrupts vascular morphology.
Emily Warren   +6 more
wiley   +1 more source

Primers for real-time RT-PCR.

open access: yes, 2013
Primers for real-time RT-PCR.
Cheng Zhang (70708)   +9 more
core   +1 more source

Self‐Sealing Microneedles Improve Precision and Reduce Reflux in Subretinal Cell Transplantation

open access: yesAdvanced Materials Technologies, EarlyView.
Ultrafine 49G microneedles enable subretinal delivery of induced pluripotent stem cell‐derived retinal pigment epithelial cells through a 50 µm outer diameter tip. Compared with conventional 38G microcannulas, these microneedles reduce cell suspension reflux in a rabbit model, highlighting the potential of microinvasive delivery instruments to improve ...
Jared Ching   +9 more
wiley   +1 more source

LN34 real-time RT-PCR protocol v1

open access: yes, 2018
Purpose To describe the LN34 real-time RT-PCR assay procedure used for the qualitative detection of lyssavirus RNA in whole RNA extracted from brain tissue samples. The assay detects RNA from diverse lyssaviruses at varying concentrations [1].
Crystal M. Gigante   +4 more
openaire   +1 more source

Primers used for real-time RT-PCR.

open access: yes, 2012
Primers used for real-time RT-PCR.
Aouatef Ait-Lounis (151054)   +1 more
core   +1 more source

Chemical Screening Identifies Danofloxacin as a Self‐Renewal Agonist of Embryonic Stem Cells through Alleviation of HDAC1‐Mediated Deacetylation of Tert and Prdm10

open access: yesAdvanced Science, EarlyView.
This study identified Danofloxacin as a novel supporter of the maintenance of mouse embryonic stem cell (ESC) pluripotency. It functions partially by inhibiting HDAC1, which increases specific histone acetylation marks and activates key genes like Tert and Prdm10.
Yan Zhang   +8 more
wiley   +1 more source

Rapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples

open access: yesArchives of Razi Institute, 2012
During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV.
Mahravani, H.   +7 more
doaj  

Real-time RT-PCR primer sequences.

open access: yes, 2013
Real-time RT-PCR primer sequences.
Kemal Kazan (134524)   +5 more
core   +1 more source

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