Results 131 to 140 of about 1,220,561 (394)

Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols

Brazilian Journal of Medical and Biological Research, 2018
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds.
F.M. de Moraes, D.L.A. Espósito, T.M. Klein, B.A.L. da Fonseca   +3 more
doaj   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Enhanced cardiac expression of two isoforms of matrix metalloproteinase-2 in experimental diabetes mellitus. [PDF]

, 2019
BackgroundDiabetic cardiomyopathy (DM CMP) is defined as cardiomyocyte damage and ventricular dysfunction directly associated with diabetes independent of concomitant coronary artery disease or hypertension.
Ahn, Jinhee, Bae, Sun Sik, Cha, Kwang Soo, Choi, Jung Hyun, Hong, Taek Jong, Kim, Sang Sik, Lee, Han Cheol, Lee, Hye Won, Lee, Min Young, Lee, Sun Ju, Lovett, David H, Oh, Jun-Hyok, Park, Jin-Sup, Park, Mi Wha, Shin, Nari, Song, Sang Heon   +15 more
core   +2 more sources

Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR

BMC Molecular Biology, 2009
BackgroundReal-time quantitative reverse transcription PCR (RT-qPCR) data needs to be normalized for its proper interpretation. Housekeeping genes are routinely employed for this purpose, but their expression level cannot be assumed to remain constant ...
Ruibo Hu, Chengming Fan, Hongyu Li, Qingzhu Zhang, Yongfu Fu   +4 more
semanticscholar   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Molecular epidemiology of human rhinovirus infections in Kilifi, coastal Kenya [PDF]

, 2012
This study reports pediatric surveillance over 3 years for human rhinovirus (HRV) at the District Hospital of Kilifi, coastal Kenya. Nasopharyngeal samples were collected from children presenting at outpatient clinic with no signs of acute respiratory ...
Agoti, Charles N., Bett, Ann, Cane, Patricia, Munywoki, Patrick K., Myers, Richard, Ngama, Mwanajuma, Nokes, D. James, Onyango, Clayton O., Welch, Stephen R.   +8 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

ISOLATION AND GENETIC IDENTIFICATION OF HUMAN NON-POLIO ENTEROVIRUSES AT THE NATIONAL REFERENCE LABORATORY OF ENTEROVIRUSES, BULGARIA

Problems of Infectious and Parasitic Diseases
Background: Human non-polio enteroviruses (NPEVs) are a diverse group of RNA viruses belonging to the genus Enterovirus within the subfamily Ensavirinae of the family Picornaviridae.
Irina Georgieva, Asya Stoyanova, Virginia Petkova, Simona Zlatanova, Ana-Maria Donova, Ivan Ivanov, Oliana Boykinova   +6 more
doaj   +1 more source

Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data

BioTechniques, 2005
Profiling studies using microarrays to measure messenger RNA (mRNA) expression frequently identify long lists of differentially expressed genes. Differential expression is often validated using real-time reverse transcription PCR (RT-PCR) assays.
Lynne V. Abruzzo, Kathleen Y. Lee, Alexandra Fuller, Alan Silverman, Michael J. Keating, L. Jeffrey Medeiros, Kevin R. Coombes   +6 more
doaj   +1 more source