Results 161 to 170 of about 1,220,561 (394)
RT real-time PCR-based quantification ofUromyces fabae in planta [PDF]
, 2011 Ralf T. Voegele, Annette C. Schmid
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ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan +9 more
wiley +1 more source
Cardiac‐Derived ECM Microspheres for Enhanced hiPSC‐CMs Maturation
Advanced Functional Materials, EarlyView.Cardiac extracellular matrix microspheres derived from decellularized porcine heart provide a biomimetic 3D microenvironment for human induced pluripotent stem cell–derived cardiomyocytes (hiPSC‐CMs). This platform supports short‐ and long‐term culture, enhances structural organization, and promotes electrophysiological and functional maturation of ...
Jiazhu Xu +9 more
wiley +1 more source
BackgroundMany cases of Fuchs\u2019 uveitis have been associated with persistent rubella virus infection. A 73-year-old male patient with typical Fuchs\u2019 Uveitis Syndrome (FUS) first experienced heterochromia of the left eye at the age fourteen, when
core
High resolution meltcurve polymerase chain reaction assay for detection of salmonella and e. coli O157:H7 in foods [PDF]
Foodborne illnesses associated with Salmonella and Escherichia coli O157:H7 have become world-wide public-health problems. Conventional methods for the identification of foodborne pathogens are tedious, expensive, and time-consuming.
Liu, Yujiao, 1990-
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Real-Time RT-PCR Detection of Equine Coronavirus
, 2016 Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and ...
Miszczak, Fabien +3 more
openaire +1 more source
Advanced Functional Materials, EarlyView.A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo +7 more
wiley +1 more source
A new mathematical model for relative quantification in real-time RT-PCR.
Nucleic Acids Research, 2001 M. Pfaffl
semanticscholar +1 more source
LN34 real-time RT-PCR protocol v2
, 2018 Purpose To describe the LN34 real-time RT-PCR assay procedure used for the qualitative detection of lyssavirus RNA in whole RNA extracted from brain tissue samples. The assay detects RNA from diverse lyssaviruses at varying concentrations [1].
Crystal M. Gigante +4 more
openaire +1 more source