Results 181 to 190 of about 1,220,561 (394)

Advanced 3D Platforms for Modeling CNS Neuroinflammation: Cell Integration, Techniques, and Challenges

Advanced Healthcare Materials, EarlyView.
This review summarizes the roles of key central nervous system cell types, the extracellular matrix, and the blood‐brain barrier in neuroinflammation, and their integration into diverse 3D culture systems. It examines major incorporation strategies, including direct co‐culture, hydrogel encapsulation, transwell migration assays, and bioprinting ...
Emmanuelle D. Aiyegbusi, Julia Di Stefano, Dearbhaile Dooley   +2 more
wiley   +1 more source

Heparinase treatment of RNA before quantitative real-time RT-PCR

BioTechniques, 2003
Mary Lynn Johnson, Chainarong Navanukraw, Anna T. Grazul-Bilska, Lawrence P. Reynolds, Dale A. Redmer   +4 more
doaj   +1 more source

Quantification of infectious recombinant murine MSCV-based retroviruses using PCR approach [PDF]

, 2010
The current available molecular method to detect the retroviral-mediated gene expression is reverse transcriptase-polymerase chain reaction (RT-PCR). However, the conventional PCR is time consuming, prone to error and not sensitive.
Allaudin, Zeenathul Nazariah, Ismail, Ruzila, Mohd Lila, Mohd Azmi, Mustafa, Nor Hidayah, Nik Abd Rahman, Nik Mohd Afizan   +4 more
core  

Comparative evaluation of single step real-time RT-PCR and gel based RT-PCR assay for detection of classical swine fever

, 2015
Gitika Rajbongshi, Nagendra Nath Barman, Elina Khatoon, Kongkon Baruah, N. C. Deka, Saurabh Das, S. Sarma, Razibuddin Ahmed Hazarika   +7 more
openalex   +2 more sources

Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.

Journal of Experimental Botany, 2005
N. Nicot, J. Hausman, L. Hoffmann, D. Evers   +3 more
semanticscholar   +1 more source

A Tri‐Culture Heart‐on‐a‐Chip Platform With iPSC‐Derived Cardiac Cells for Predictive Cardiotoxicity Testing

Advanced Healthcare Materials, EarlyView.
This study presents the first entirely isogenic heart‐on‐chip, unifying cardiomyocytes, fibroblasts, and endothelial cells from a single iPSC source. The platform reveals a critical biological insight: the endothelium actively shields cardiac tissue from drug‐induced toxicity, challenging the predictive accuracy of conventional, avascular models for ...
Karine Tadevosyan, Jose Yeste, Mar Alvarez, Denise Marrero, Laura Casado‐Medina, Yvonne Richaud‐Patin, Olalla García‐Iglesias, Jagoda Litowczenko, Alba Morillas Garcia, Anton Guimerà, Xavi Illa, Rosa Villa, Angel Raya   +12 more
wiley   +1 more source

Rubella virus real-time RT-PCR v1

, 2018
A real-time assay for the detection of Rubella virus RNA from clinical samples. This assay was modified from a published method with oligonucleotides updated, a different kit used, and cycling times adjusted accordingly.
Ian M Mackay, Judy A Northill
openaire   +1 more source

Extracellular Vesicles Modulation by an Adiponectin Receptor Agonist Provides Cardioprotection for Myocardial Ischemic Injury

Advanced Healthcare Materials, EarlyView.
This study demonstrates that ALY688, a drug mimicking the heart‐protective hormone adiponectin, reduces myocardial ischemia injury. ALY688 increases the production of extracellular vesicles, which carry protective cargo including adiponectin itself.
Jialing Tang, Wing Yan Chung, Serena M. Pulente, Yubin Lei, Khang Nguyen, Eddie Tam, Nadya M. Morrow, Ilka Lorenzen‐Schmidt, Fengxia Xiao, Augusto Zani, Dylan Burger, Hye Kyoung Sung, Erin E. Mulvihill, Gary Sweeney   +13 more
wiley   +1 more source

Real-Time Quantitative Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for the Measurement of Prostate-Specific Antigen mRNA in the Peripheral Blood of Patients with Prostate Carcinoma Using the TaqManTM Detection System

, 2001
Stefania Gelmini, Carmela Tricarico, Giovanna Vona, Lorenzo Livi, Alessandro Della Melina, Sergio Serni, E. Cellai, Stefano Maria Magrini, Donata Villari, Marco Carini, Mario Serio, Gianni Forti, Mario Pazzagli, Claudio Orlando   +13 more
openalex   +2 more sources

Development of real-time RT-PCR for human metapneumovirus

Acta Virologica, 2011
P, Xue, W, Zheng, M, Zhang, L, Zheng
openaire   +2 more sources