Results 191 to 200 of about 637,318 (299)

Diagnostic utility of real-time RT-PCR for chikungunya virus detection in the acute phase of infection: a retrospective study. [PDF]

Ann Med
Sajith A, Iyengar V, Varamballi P, Mukhopadhyay C, Nittur S.   +4 more
europepmc   +1 more source

Time‐Multiplexed Organic Electrochemical Transistor for Saliva‐Based Rapid Detection of Viral Proteins

Advanced Science, EarlyView.
A time‐multiplexed nanobody‐functionalized organic electrochemical transistor enables rapid and sensitive detection of three respiratory viral proteins from saliva. Sequentially addressed multi‐gate electrodes on a shared channel achieve around 1 fm detection within 15 min without sample preprocessing. Clinical validation demonstrates high specificity,
Tianrui Chang, Yuxiang Ren, Shofarul Wustoni, Atheer Alqatari, Adel Hama, Yazhou Wang, Long Chen, Jessica Parrado Agudelo, Luca Salvigni, Keying Guo, Ashraf Dada, Stefan T. Arold, Raik Grünberg, Sahika Inal   +13 more
wiley   +1 more source

Development and Evaluation of a New Measles Detection Assay Using Real-Time RT-PCR. [PDF]

Int J Mol Sci
Chayeb VA, Dolgova AS, Popova MR, Zheleznova NV, Shirobokova SA, Shabalina AV, Sharova AA, Gladkikh AS, Antipova AY, Kirichenko AD, Ramsay ES, Dedkov VG.   +11 more
europepmc   +1 more source

G3BP1 Succinylation at K413 is Critical for Cardiac Function by Modulating PI3K‐AKT‐mTOR Signal Axis

Advanced Science, EarlyView.
Schematic illustrating the impact of G3BP1 succinylation at K413 on cardiac function. In the healthy human heart, G3BP1 succinylation maintains homeostatic mTOR signaling. In patients with dilated cardiomyopathy (DCM) and heart failure (HF), G3BP1 de‐succinylation induces RagA expression and disrupts the binding of the TSC1/2 complex, leading to the ...
Yuan Zhang, Cancan Yao, Yan Chen, Ke Cai, Zhouping Lu, Boxuan Wu, Kun Yu, Yan Shi, Jianyuan Zhao, Xiangyu Zhou   +9 more
wiley   +1 more source

Development of a Multiplex TaqMan Real-Time RT-PCR Assay for the Rapid Differential Detection of Classic, MLB-Clade and VA-Clade Human Astroviruses. [PDF]

J Microbiol Biotechnol
Zheng P, Wang J, Yu H, Luo Q, Gao H, Jin Z, Shi Y, Wang H, Li P, Tian F.   +9 more
europepmc   +1 more source

Tumor‐Derived Alpha‐1 Antitrypsin Promotes Liver Metastasis of Colorectal Cancer Through the Neutrophil Extracellular Traps–CCDC25 Pathway

Advanced Science, EarlyView.
ABSTRACT Liver metastasis is a leading cause of mortality in colorectal cancer (CRC), where the inflammatory tumor microenvironment, specifically neutrophil infiltration, significantly promotes metastatic colonization. This study reveals a pro‐metastatic role for alpha‐1 antitrypsin (A1AT) in CRC liver metastasis via a dual mechanism involving ...
Qian Fei, Chunning Li, Lei Zhan, Xiaoyan Li, Yan Hui, Yue Jin, Jing Zhang, Lu Yang, Xin Sun, Xiaoxi Li, Qian Dong, Jingdong Zhang   +11 more
wiley   +1 more source

DIFFERENTIAL DIAGNOSIS OF RESPIRATORY VIRUSES BY USING REAL TIME RT-PCR METHODOLOGY

Revista do Instituto de Medicina Tropical de São Paulo, 2013
Renato de Souza Paulino, Margarete Aparecida Benega, Katia Correa de Oliveira Santos, Daniela Bernardes Borges da Silva, Juliana Cristina Pereira, Norio Augusto Sasaki, Patricia Evelin Silva, Suely Pires Curti, Maria Isabel Oliveira, Telma R.M.P. Carvalhanas, Teresa Peret, Dean Erdman, Terezinha Maria de Paiva   +12 more
doaj   +1 more source

Optimization and Validation of Universal Real-Time RT-PCR Assay to Detect Virulent Newcastle Disease Viruses. [PDF]

Viruses
Alexander Morris ER, Schroeder ME, Anderson PN, Schroeder LJ, Monday N, Senties-Cue G, Ficken M, Ferro PJ, Suarez DL, Dimitrov KM.   +9 more
europepmc   +1 more source

Tumor‐Derived LAMB3 Drives Immunosuppressive LRRC15+ Fibroblast Formation During Pancreatic Ductal Adenocarcinoma Development

Advanced Science, EarlyView.
A single‐cell atlas of pancreatic ductal adenocarcinoma development reveals progressive ductal‐fibroblast‐immune crosstalk. Tumor‐derived LAMB3 drives the formation of immunosuppressive LRRC15+ fibroblasts through the ITGB1/FAK/MAPK/FOSL2 signaling. Glycolytic reprogramming upregulates LAMB3 and correlates with LRRC15+ fibroblast enrichment.
Xuqing Shi, Hangqi Liu, Jianru Sun, Xiaoding Liu, Xinping Jv, Longyun Chen, Yuhan Zhang, Hui Zhang, Xudong Xing, Ruiyu Li, Xinyi Ke, Jun Wang, Xianglin Yin, Bohan Liu, Qixian Liu, Yuan Wang, Junliang Lu, Shiyi Liu, Junyi Pang, Yumeng Cai, Menghua Dai, Fan Bai, Huanwen Wu, Zhiyong Liang   +23 more
wiley   +1 more source

Analytical performance evaluation of a multiplex real-time RT-PCR kit for simultaneous detection of SARS-CoV-2, influenza A/B, and RSV. [PDF]

PeerJ
Tai CS, Jian MJ, Lin TH, Chung HY, Chang CK, Perng CL, Hsieh PS, Shang HS.   +7 more
europepmc   +1 more source